GapMind for catabolism of small carbon sources

 

Aligments for a candidate for natA in Sinorhizobium meliloti 1021

Align NatA aka BRAF aka SLR0467, component of Leucine/proline/alanine/serine/glycine (and possibly histidine) porter, NatABCDE (characterized)
to candidate SMc01949 SMc01949 high-affinity branched-chain amino acid ABC transporter ATP-binding protein

Query= TCDB::Q55164
         (267 letters)



>lcl|FitnessBrowser__Smeli:SMc01949 SMc01949 high-affinity
           branched-chain amino acid ABC transporter ATP-binding
           protein
          Length = 295

 Score =  163 bits (412), Expect = 4e-45
 Identities = 101/259 (38%), Positives = 149/259 (57%), Gaps = 12/259 (4%)

Query: 18  LLLAQGLSKSFGGLRAVDHADIVVKEGSITGLIGPNGAGKTTLFNLLSNFIRPDQGEVLF 77
           +L  + LS  FGGL A++      + G IT LIGPNGAGKTT+FN ++ F +P  G +  
Sbjct: 13  ILKVERLSMRFGGLMAINDFSFEAERGEITALIGPNGAGKTTVFNCITGFYKPTMGMITM 72

Query: 78  NGDS-----IGQLAPHQIALRGSV-RTFQVAKVLSRLTVLENMLLADQHQ----TGEKFL 127
              S     + +L   +I  +  V RTFQ  ++ S LTVLEN+L+A  ++    +G   L
Sbjct: 73  RQKSGAEFLLERLPDFEITKKAKVARTFQNIRMFSGLTVLENLLVAQHNKLMRASGYTIL 132

Query: 128 PRLINFRRVQKEERANREKAMAMLESVGLGAKAQDYAGALSGGQRKLLEMARALMSNPKL 187
             L  F   ++  R + E A   LE   L  +A D AG L  G ++ LE+ARA+ + P+L
Sbjct: 133 -GLFGFPAYREASRESIELARHWLEKASLTERADDPAGDLPYGAQRRLEIARAMCTGPEL 191

Query: 188 ILLDEPAAGVNPTLIGQICEHIVNWNRQ-GITFLVIEHNMDVIMTLCHHVWVLAEGRNLA 246
           + LDEPAAG+NP     +   +    R  G + L+IEH+M V+M +  HV VL  G+ ++
Sbjct: 192 LCLDEPAAGLNPRESLALNALLQEIRRDTGTSILLIEHDMSVVMEISDHVVVLEYGQKIS 251

Query: 247 DGTPEQIQSDPRVLEAYLG 265
           DG P+ +++DPRV+ AYLG
Sbjct: 252 DGNPDFVKNDPRVIAAYLG 270


Lambda     K      H
   0.319    0.136    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 183
Number of extensions: 8
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 267
Length of database: 295
Length adjustment: 26
Effective length of query: 241
Effective length of database: 269
Effective search space:    64829
Effective search space used:    64829
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory