Align galactose-6-phosphate isomerase lacB subunit (EC 5.3.1.26) (characterized)
to candidate SM_b20371 SM_b20371 ribose-5-phosphate isomerase B
Query= metacyc::MONOMER-2782 (171 letters) >FitnessBrowser__Smeli:SM_b20371 Length = 148 Score = 106 bits (265), Expect = 2e-28 Identities = 51/145 (35%), Positives = 84/145 (57%) Query: 1 MKIALGCDHIVTDTKMRVSEFLKSKGHEVIDVGTYDFTRTHYPIFGKKVGEQVVSGNADL 60 M+I +G DH K + + + S GHEV D G+YD +P K V ++ G AD Sbjct: 1 MRIVIGSDHAGFHLKATIIDHVTSLGHEVYDAGSYDANPVDFPDIAKMVTSSIIEGKADR 60 Query: 61 GVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNANVIGFGGRIIGELLMCDII 120 G+ +CGTGVG + A NKV G+R+A+ D+ SA + E + NV+ G +I+G L D++ Sbjct: 61 GLMVCGTGVGASIAANKVKGIRAAVCHDVHSAHQSVEHDDVNVMCIGAQIVGAWLARDLV 120 Query: 121 DAFINAEYKPTEENKKLIAKIKHLE 145 A++ AE+ E+ ++ + K+ ++ Sbjct: 121 AAYLAAEFSTDEDFRRRVRKLAEMD 145 Lambda K H 0.320 0.139 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 76 Number of extensions: 1 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 171 Length of database: 148 Length adjustment: 17 Effective length of query: 154 Effective length of database: 131 Effective search space: 20174 Effective search space used: 20174 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 43 (21.2 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory