Aligments for a candidate for bkdA in Sinorhizobium meliloti 1021

GapMind for catabolism of small carbon sources

Aligments for a candidate for bkdA in Sinorhizobium meliloti 1021

Align 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) (EC 1.2.4.4) (characterized)
to candidate SMc03201 SMc03201 2-oxoisovalerate dehydrogenase subunit alpha

Query= reanno::Smeli:SMc03201
         (410 letters)



>FitnessBrowser__Smeli:SMc03201
          Length = 410

 Score =  830 bits (2145), Expect = 0.0
 Identities = 410/410 (100%), Positives = 410/410 (100%)

Query: 1   MDEFSRLSLHVPEPAVRPGDLPDFSNVKIPKAGSVPRPDVDVDPEEIRDLAYSIIRVLNR 60
           MDEFSRLSLHVPEPAVRPGDLPDFSNVKIPKAGSVPRPDVDVDPEEIRDLAYSIIRVLNR
Sbjct: 1   MDEFSRLSLHVPEPAVRPGDLPDFSNVKIPKAGSVPRPDVDVDPEEIRDLAYSIIRVLNR 60

Query: 61  EGEAVGPWAGFLSDEELLTGLRHMMLLRAFDARMLMAQRQGKTSFYMQHLGEEAVSCAFR 120
           EGEAVGPWAGFLSDEELLTGLRHMMLLRAFDARMLMAQRQGKTSFYMQHLGEEAVSCAFR
Sbjct: 61  EGEAVGPWAGFLSDEELLTGLRHMMLLRAFDARMLMAQRQGKTSFYMQHLGEEAVSCAFR 120

Query: 121 KALRKGDMNFPTYRQAGLLIADDYPMVEMMNQIFSNELDPCHGRQLPVMYTSKEHGFFTI 180
           KALRKGDMNFPTYRQAGLLIADDYPMVEMMNQIFSNELDPCHGRQLPVMYTSKEHGFFTI
Sbjct: 121 KALRKGDMNFPTYRQAGLLIADDYPMVEMMNQIFSNELDPCHGRQLPVMYTSKEHGFFTI 180

Query: 181 SGNLATQYVQAVGWAMASAIKNDTRIAAGWIGDGSTAESDFHSALVFASTYKAPVILNIV 240
           SGNLATQYVQAVGWAMASAIKNDTRIAAGWIGDGSTAESDFHSALVFASTYKAPVILNIV
Sbjct: 181 SGNLATQYVQAVGWAMASAIKNDTRIAAGWIGDGSTAESDFHSALVFASTYKAPVILNIV 240

Query: 241 NNQWAISTFQGIARGGSGTFAARGLGFGIPALRVDGNDYLAVYAVARWAAERARLNLGPT 300
           NNQWAISTFQGIARGGSGTFAARGLGFGIPALRVDGNDYLAVYAVARWAAERARLNLGPT
Sbjct: 241 NNQWAISTFQGIARGGSGTFAARGLGFGIPALRVDGNDYLAVYAVARWAAERARLNLGPT 300

Query: 301 LIEYVTYRVGAHSTSDDPSAYRPKTESEAWPLGDPVLRLKKHLILRGAWSEERHAQAEAE 360
           LIEYVTYRVGAHSTSDDPSAYRPKTESEAWPLGDPVLRLKKHLILRGAWSEERHAQAEAE
Sbjct: 301 LIEYVTYRVGAHSTSDDPSAYRPKTESEAWPLGDPVLRLKKHLILRGAWSEERHAQAEAE 360

Query: 361 IMDEVIQAQKEAERHGTLHAGGRPSVRDIFEGVYAEMPPHIRRQRQKAGY 410
           IMDEVIQAQKEAERHGTLHAGGRPSVRDIFEGVYAEMPPHIRRQRQKAGY
Sbjct: 361 IMDEVIQAQKEAERHGTLHAGGRPSVRDIFEGVYAEMPPHIRRQRQKAGY 410


Lambda     K      H
   0.320    0.135    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 794
Number of extensions: 25
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 410
Length of database: 410
Length adjustment: 31
Effective length of query: 379
Effective length of database: 379
Effective search space:   143641
Effective search space used:   143641
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources