GapMind for catabolism of small carbon sources

 

Alignments for a candidate for fadB in Sinorhizobium meliloti 1021

Align 3-hydroxyacyl-CoA dehydrogenase type-2; 17-beta-hydroxysteroid dehydrogenase 10; 17-beta-HSD 10; 3-hydroxy-2-methylbutyryl-CoA dehydrogenase; 3-hydroxyacyl-CoA dehydrogenase type II; Mitochondrial ribonuclease P protein 2; Mitochondrial RNase P protein 2; Scully protein; Type II HADH; EC 1.1.1.35; EC 1.1.1.51; EC 1.1.1.178 (characterized)
to candidate SMa1452 SMa1452 hypothetical protein

Query= SwissProt::O18404
         (255 letters)



>FitnessBrowser__Smeli:SMa1452
          Length = 255

 Score =  267 bits (682), Expect = 2e-76
 Identities = 140/254 (55%), Positives = 179/254 (70%), Gaps = 2/254 (0%)

Query: 2   IKNAVSLVTGGASGLGRATAERLAKQGASVILADLPSSKGNEVAKELGDKVVFVPVDVTS 61
           +K+ V +VTG +SGLG A    LA++GA+V+  DL    G E A ELG  V F   DVT+
Sbjct: 3   LKSRVFIVTGASSGLGAAVTRMLAQEGATVLGLDLKPPAGEEPAAELGAAVRFRNADVTN 62

Query: 62  EKDVSAALQTAKDKFGRLDLTVNCAGTATAVKTFNFNKNVAHRLEDFQRVININTVGTFN 121
           E D +AAL  AK +FG +   VNCAGTA   K     ++  H L+ F R + +N +GTFN
Sbjct: 63  EADATAALAFAKQEFGHVHGLVNCAGTAPGEKILG--RSGPHALDSFARTVAVNLIGTFN 120

Query: 122 VIRLSAGLMGANEPNQDGQRGVIVNTASVAAFDGQIGQAAYSASKAAVVGMTLPIARDLS 181
           +IRL+A +M   EP+ DG+RGVIVNTAS+AAFDGQIGQAAY+ASK  V  +TLP AR+L+
Sbjct: 121 MIRLAAEVMSQGEPDADGERGVIVNTASIAAFDGQIGQAAYAASKGGVAALTLPAARELA 180

Query: 182 TQGIRICTIAPGLFNTPMLAALPEKVRTFLAKSIPFPQRLGEPSEYAHLVQAIYENPLLN 241
             GIR+ TIAPG+F+TPM+A +P+ V+  LA S+PFP RLG   EYA LV+ I EN +LN
Sbjct: 181 RFGIRVVTIAPGIFDTPMMAGMPQDVQDALAASVPFPPRLGRAEEYAALVKHICENTMLN 240

Query: 242 GEVIRIDGALRMMP 255
           GEVIR+DGALRM P
Sbjct: 241 GEVIRLDGALRMAP 254


Lambda     K      H
   0.317    0.133    0.369 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 241
Number of extensions: 9
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 255
Length of database: 255
Length adjustment: 24
Effective length of query: 231
Effective length of database: 231
Effective search space:    53361
Effective search space used:    53361
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory