Align monosaccharide-transporting ATPase (EC 3.6.3.17) (characterized)
to candidate SMc01608 SMc01608 ABC transporter ATP-binding protein
Query= BRENDA::Q97UY8 (353 letters) >FitnessBrowser__Smeli:SMc01608 Length = 353 Score = 212 bits (540), Expect = 1e-59 Identities = 118/288 (40%), Positives = 177/288 (61%), Gaps = 16/288 (5%) Query: 1 MVRIIVKNVSKVFKKGKVVALDNVNINIENGERFGILGPSGAGKTTFMRIIAGLDVPSTG 60 M + + N+ K F G V + N ++ I+ GE LGPSG GKTT +R+IAG + PS G Sbjct: 1 MAFLQLTNIQKSF--GPVQVVHNFDMGIDKGEFVSFLGPSGCGKTTILRMIAGFETPSGG 58 Query: 61 ELYFDDRLVASNGKLIVPPEDRKIGMVFQTWALYPNLTAFENIAFPLTNMKMSKEEIRKR 120 ++ D + G L P R IGMVFQ +AL+PN+ +N+AF L S+ EI R Sbjct: 59 SIFIDGK---DQGAL--KPNQRNIGMVFQAYALFPNMNVHDNVAFGLKVAGASRTEIEAR 113 Query: 121 VEEVAKILDIHHVLNHFPRELSGGQQQRVALARALVKDPSLLLLDEPFSNLDARMRDSAR 180 V+++ ++ + H+ + FP +LSGGQQQRVALARAL P +LLLDEP S LDA++R S R Sbjct: 114 VKQMLGLIKLEHLADRFPYQLSGGQQQRVALARALAVKPQVLLLDEPLSALDAKIRISLR 173 Query: 181 ALVKEVQSRLGVTLLVVSHDPADIFAIADRVGVLVKGKLVQVGKPEDLYDNPVSIQVASL 240 ++++Q +LG+T + V+HD + +I+DR+ V+ G+ Q+G P ++Y+ P + VAS Sbjct: 174 EEIRQIQQQLGITTVFVTHDQEEALSISDRIVVMNSGRADQIGTPFEIYNTPATRFVASF 233 Query: 241 IGEINELEGKVTNE---GVVIG----SLRFPV--SVSSDRAIIGIRPE 279 +G +N +EGKV + V IG SL+ P+ + D + +RPE Sbjct: 234 VGTLNIIEGKVADPASGAVTIGGQRISLKEPIAGAKGGDSISLALRPE 281 Lambda K H 0.319 0.139 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 308 Number of extensions: 10 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 353 Length of database: 353 Length adjustment: 29 Effective length of query: 324 Effective length of database: 324 Effective search space: 104976 Effective search space used: 104976 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory