GapMind for catabolism of small carbon sources

 

Aligments for a candidate for Ch1CoA in Sinorhizobium meliloti 1021

Align Cyclohex-1-ene-1-carbonyl-CoA dehydrogenase; Ch1CoA; EC 1.3.8.10 (characterized)
to candidate SM_b20753 SM_b20753 acyl-CoA dehydrogenase

Query= SwissProt::Q2LQN9
         (414 letters)



>lcl|FitnessBrowser__Smeli:SM_b20753 SM_b20753 acyl-CoA
           dehydrogenase
          Length = 380

 Score =  249 bits (636), Expect = 1e-70
 Identities = 139/377 (36%), Positives = 215/377 (57%), Gaps = 6/377 (1%)

Query: 37  LTEEQKLLMEMVRNLAVREIAPRAIEIDENHSFPVHARDLFADLGLLSPLVPVEYGGTGM 96
           L+EEQ+ +  M  + A  EIAP A++ D+   FPV      A LG+    +  + GGTG+
Sbjct: 5   LSEEQEAIRTMALDFARDEIAPHAVDWDQQKHFPVETLRAAAALGMAGIYIRDDVGGTGL 64

Query: 97  DITTFAMVLEEIGKVCASTALMLLAQADGMLSIILD--GSPALKEKYLPRFGEKSTLMTA 154
                AM++E +   C + A  +      M + ++D  G+   + + LP       L  +
Sbjct: 65  TRLDAAMIIEALATGCPAIASFV--SIHNMCAGMIDRYGTDEQRRRLLPPLLTMDVL-AS 121

Query: 155 FAATEPGAGSDLLAMKTRAVKKGDKYVINGQKCFITNGSVADILTVWAYTDPSKGAKGMS 214
           +  TEPG+GSD  A+KTRAV++GD Y++ GQK FI+    + +  V A T   +G KG+S
Sbjct: 122 YCLTEPGSGSDAAALKTRAVREGDAYLLTGQKQFISGAGESGLYIVMARTG-EEGPKGIS 180

Query: 215 TFVVERGTPGLIYGHNEKKMGMRGCPNSELFFEDLEVPAENLVGEEGKGFAYLMGALSIN 274
            FVVE+  PGL +G NEKKMG    P   +  +++ V  EN +G EG+GF   M  L   
Sbjct: 181 AFVVEKDAPGLTFGANEKKMGWHAQPTRAVMLDNVRVSVENRLGAEGEGFRIAMAGLDGG 240

Query: 275 RVFCASQAVGIAQGALERAMQHTREREQFGKPIAHLTPIQFMIADMATEVEAARLLVRKA 334
           R+  A+ ++G AQ A ++A+ + +ER  FGK I     +QF +ADMAT++E AR  + +A
Sbjct: 241 RLSIAAASLGGAQSAFDKALAYVQERRAFGKAIGEFQALQFRLADMATDLEIARTFLWRA 300

Query: 335 TTLLDAKDKRGPLIGGMAKTFASDTAMKVTTDAVQVMGGSGYMQEYQVERMMREAKLTQI 394
              LDA D     +  MAK F +D    V  DA+Q+ GG GY+ +Y VE+++R+ ++ QI
Sbjct: 301 ACALDAADPEATKLCAMAKRFVTDRCFSVANDALQLHGGYGYLADYGVEKIVRDLRVHQI 360

Query: 395 YTGTNQITRMVTGRSLL 411
             GTN+I R++  R+++
Sbjct: 361 LEGTNEIMRLIVSRAIM 377


Lambda     K      H
   0.318    0.133    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 345
Number of extensions: 11
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 414
Length of database: 380
Length adjustment: 31
Effective length of query: 383
Effective length of database: 349
Effective search space:   133667
Effective search space used:   133667
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory