Alignments for a candidate for pimF in Sinorhizobium meliloti 1021

GapMind for catabolism of small carbon sources

Alignments for a candidate for pimF in Sinorhizobium meliloti 1021

Align 6-carboxyhex-2-enoyl-CoA hydratase (characterized)
to candidate SMc02227 SMc02227 fatty oxidation complex enoyl-COA hydratase/3-hydroxyacyl-COA dehydrogenase/3-hydroxybutyryl-COA epimerase

Query= metacyc::MONOMER-20678
         (699 letters)



>FitnessBrowser__Smeli:SMc02227
          Length = 737

 Score =  312 bits (799), Expect = 4e-89
 Identities = 230/731 (31%), Positives = 348/731 (47%), Gaps = 61/731 (8%)

Query: 11  DQVAIVTVDSPP--VNALSAAVRRGILENVNAAVADPAVQAIVLVCAGRTFIAGADIT-- 66
           D +A+VT D P   +N  +  V + +   ++   ADPAV+ +V+     +F  GAD++  
Sbjct: 13  DGIALVTWDMPDKSMNVFTEEVMKELDAIIDQTTADPAVKGVVITSGKSSFSGGADLSMI 72

Query: 67  ---------EFGKPPQPPA---------LNDVIAALENSPKPTIAAIHGTALGGGLEVAL 108
                    E  K P   A         +  +   LE S KP ++AI+GT +GG  E++L
Sbjct: 73  KSMFTFQAEERKKDPDNAARKLFDLVGRMTGLFRKLETSGKPWVSAINGTCMGGAFEMSL 132

Query: 109 GCHFRVA--VKEAKLGLPEVKLGLLPGAGGTQRLPRAVGPELAVQMIVGGSPIGAAEALK 166
            CH RVA   K  K+ LPEVK+G+ PGAGGTQR+PR    + A+QM+  GS +  A A  
Sbjct: 133 ACHGRVASNAKSVKIALPEVKVGIFPGAGGTQRVPRLTNTQDALQMMTTGSSLTPARAKA 192

Query: 167 HGLVEEVVE--NLVAGAVAFAKKVLAEKRPLRRLRDDDSKLAAAKADRSIFTNAVAAMTK 224
            GLV EVV+   L+  A A  K  L   +P++   +   KL        I+T A A +  
Sbjct: 193 MGLVHEVVDPDKLIEAAKAMIKNGL---KPVQPWDEKGFKLPGG----GIWTPASAQLWP 245

Query: 225 KARGL---------EAPFACADAIGAAIDLPFEEGLKKEREGFMKLVVSDQSKAQ-RYAF 274
            A G+             A    +   + +PF+  LK E+  F +++ + ++ +  R  F
Sbjct: 246 AASGILRRETYGNYPGAIAILKCVYEGLQVPFDTALKIEQRYFTEILQTTEAFSMIRSLF 305

Query: 275 FAEREAAKVDGVPDGTKPRPVSRVAIIGAGTMGGGIAMSFANAGIPVTLIETGEEQLKRG 334
            + +E  K    P G     + +V ++GAG MG  IA   A  GIPVTLI+   E  ++G
Sbjct: 306 VSMQELGKGARRPAGVPKTELKKVGVVGAGFMGASIAYVTAAGGIPVTLIDRDMEAAEKG 365

Query: 335 LGIMQKNWEATAARGGLPPDAPAKRMALITGLVGLENVKDADLIIEAVFETMAVKKEVFT 394
               +   +    +G L  +     ++ IT      +++DA L+IEAVFE   VKK+V  
Sbjct: 366 KTHSEGLVKDAIGKGRLTKEEGEALLSRITPSADYGDLRDAGLVIEAVFEDRQVKKDVIE 425

Query: 395 AVDAHAKPGAVLASNTSYLSIDEIAATTKRPQDVLGMHFFSPANVMKLCEIVRGAKTAPD 454
            V+A     A+ ASNTS L I  +A  +KRP   +G+HFFSP   M L E++ G +T   
Sbjct: 426 KVEAVIAEDAIFASNTSTLPITGLARNSKRPDRFIGIHFFSPVEKMMLTEVILGKETGDR 485

Query: 455 ALLTAVSIAKKIAKVPVVVGVCDGFVGNRMLAARSKQSEKLLFEGALPQQVDAVVTKFGM 514
           AL  A+     I K P+VV    GF  NR +     ++  +L EG  P  ++      GM
Sbjct: 486 ALAAALDYVAAIRKTPIVVNDTRGFYVNRCVFRYIHEAYDMLIEGVPPAMIENAAKMAGM 545

Query: 515 PMGPFAMGDLAGLDIGWRSRK----DRGIKSE-------IADALCEAGRFGQKTGKGYYK 563
           P+GP ++ D   +D+  +  K    D G K+        I   + E  R G+K GKG+Y+
Sbjct: 546 PVGPLSLNDEVAIDLSQKILKAAVADLGDKAVDLRHMALIDKMVDELDRRGRKNGKGFYE 605

Query: 564 YEQGSRAPMPDPEVETLINDTLAKLGLKRRDITDEEILERMVYPMINEGARILEEKIAAR 623
           Y          P ++ L     A       +I    + +R++  +  E AR +EE I   
Sbjct: 606 YPAKPARKYLWPGLKELYPQKDA------GEIEVGVLKQRLLVTIALEAARTIEEGIVTD 659

Query: 624 PSDIDVVWLYGYGWPIYRGGPMHYADSVGLKHIAERLSAYAKATNDPSLEPAPLLARLAA 683
           P + DV  + G+G+  Y GG + Y D +G K   E     A A +     P PLL  +A 
Sbjct: 660 PREADVGSILGFGFAPYTGGTLSYIDGMGAKAFVELCEKLA-ADHGDHFRPTPLLKEMAE 718

Query: 684 EGKTFASLTQP 694
           +G+TF     P
Sbjct: 719 KGETFYGRFDP 729


Lambda     K      H
   0.317    0.134    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1127
Number of extensions: 68
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 699
Length of database: 737
Length adjustment: 40
Effective length of query: 659
Effective length of database: 697
Effective search space:   459323
Effective search space used:   459323
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources