Alignments for a candidate for dddA in Sinorhizobium meliloti 1021

GapMind for catabolism of small carbon sources

Alignments for a candidate for dddA in Sinorhizobium meliloti 1021

Align 3-hydroxypropionate dehydrogenase (EC 1.1.1.59) (characterized)
to candidate SM_b20496 SM_b20496 GMC oxidoreductase

Query= metacyc::MONOMER-15202
         (579 letters)



>FitnessBrowser__Smeli:SM_b20496
          Length = 536

 Score =  615 bits (1587), Expect = e-180
 Identities = 307/542 (56%), Positives = 385/542 (71%), Gaps = 17/542 (3%)

Query: 35  AFDYIVVGAGTAGCLLANRLSADPANRVLLIEAGGRDNYHWIHIPVGYLYCINNPRTDWR 94
           ++D+IV+GAG+AGC+LANRLSADP NRVLL+EAGG D YHW+H+P+GYLYC+ NPRTDW 
Sbjct: 10  SYDFIVIGAGSAGCVLANRLSADPKNRVLLLEAGGSDRYHWVHVPIGYLYCMGNPRTDWM 69

Query: 95  FRTEPDPGLNGRSLIYPRGKTLGGCSSINGMLYLRGQARDYDGWAELTGDDAWRWDNCLP 154
            RT  +PGLNGRSL YPRGK LGGCSSINGM+Y+RGQA DYDGW +  G+  W WD+ LP
Sbjct: 70  MRTAAEPGLNGRSLPYPRGKLLGGCSSINGMIYMRGQAADYDGWRQ-AGNTGWGWDDVLP 128

Query: 155 DFMRHEDHYRLDEGGDADPDHYKFHGHGGEWRIEKQRLKWQVLADFATAAVEAGVPRTRD 214
            F++ ED++R              HG GGEWR+E+QRL W +L  F  AA E G+P+  D
Sbjct: 129 YFLKSEDNFR---------GKSPMHGAGGEWRVERQRLSWPILDAFRDAAEELGIPKAED 179

Query: 215 FNRGDNEGVDAFEVNQRSGWRWNASKAFLRGVEQRGNLTVWHSTQVLKLDFASGEGSEPR 274
           FN GDNEG   FEVNQR G RWN SKAFLR   +R NL V    +  +L F        R
Sbjct: 180 FNTGDNEGSGYFEVNQRGGVRWNTSKAFLRPAMKRPNLRVLTGAETERLIFDGR-----R 234

Query: 275 CCGVTVERAGKKVVTTARCEVVLSAGAIGSPQLLQLSGIGPTALLAEHAIPVVADLPGVG 334
             GV     G+  V  A  EV+LSAGAI SP++L+LSG+G   +++     VV DLPGVG
Sbjct: 235 TKGVRFRLNGRIQVARATREVILSAGAINSPKILELSGVGRPDVVSAAGAEVVHDLPGVG 294

Query: 335 ENLQDHLQIRSIYKVKGAKTLNTMANSLIGKAKIGLEYILKRSGPMSMAPSQLCIFTRSS 394
           ENLQDHLQIR++++++GAKTLN + +S+  +  +G EY+L+RSGP+SMAP QL IF +SS
Sbjct: 295 ENLQDHLQIRTVFRIEGAKTLNQLYHSIFSRIGMGAEYMLRRSGPLSMAPRQLGIFAKSS 354

Query: 395 KEYEHPNLEYHVQPLSLEAFGQPLHDFPAITASVCNLNPTSRGTVRIKSGNPRQAPAISP 454
                 +LEYHVQPLS +  G+PLH +PA+T SVCNL P SRG+V + +     AP I P
Sbjct: 355 PSVATADLEYHVQPLSTDRLGEPLHRYPAVTVSVCNLRPESRGSVHVTTAESSAAPDIRP 414

Query: 455 NYLSTEEDRQVAADSLRVTRHIASQPAFAKYDPEEFKPGVQYQSDEDLARLAGDIGTTIF 514
           NYLST  DR +AA ++R  R++ +  A A++ P E  PG ++QS+++L R AGDI TTIF
Sbjct: 415 NYLSTPGDRLLAAHAIRHARNLMATKAIARFRPAEMLPGSEFQSEDELIRRAGDIATTIF 474

Query: 515 HPVGTAKMGRDDDPMAVVDSHLRVRGVTGLRVVDASIMPTITSGNTNSPTLMIAEKAAGW 574
           HPVGT KMG   DPMAVVD  L+V G+ GLRVVDASIMP+I SGNTNSP +MIAEKAA  
Sbjct: 475 HPVGTCKMG--TDPMAVVDPSLKVHGLDGLRVVDASIMPSIVSGNTNSPVIMIAEKAAEA 532

Query: 575 IL 576
           IL
Sbjct: 533 IL 534


Lambda     K      H
   0.318    0.135    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 947
Number of extensions: 45
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 579
Length of database: 536
Length adjustment: 36
Effective length of query: 543
Effective length of database: 500
Effective search space:   271500
Effective search space used:   271500
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources