Aligments for a candidate for mcmA in Sinorhizobium meliloti 1021

GapMind for catabolism of small carbon sources

Aligments for a candidate for mcmA in Sinorhizobium meliloti 1021

Align Methylmalonyl-CoA mutase; MCM; EC 5.4.99.2 (characterized)
to candidate SM_b20757 SM_b20757 methylmalonyl-CoA mutase

Query= SwissProt::O86028
         (712 letters)



>FitnessBrowser__Smeli:SM_b20757
          Length = 712

 Score = 1389 bits (3595), Expect = 0.0
 Identities = 712/712 (100%), Positives = 712/712 (100%)

Query: 1   MTEKTIKDWEALAEKELRVSPEGLVWHTPEGIDVKPLYTSDDMSGIGHLNSLPGFEPFVR 60
           MTEKTIKDWEALAEKELRVSPEGLVWHTPEGIDVKPLYTSDDMSGIGHLNSLPGFEPFVR
Sbjct: 1   MTEKTIKDWEALAEKELRVSPEGLVWHTPEGIDVKPLYTSDDMSGIGHLNSLPGFEPFVR 60

Query: 61  GPRATMYAGRPWTVRQYAGFSTAEASNAFYRRNLAAGQQGVSVAFDLATHRGYDSDHPRV 120
           GPRATMYAGRPWTVRQYAGFSTAEASNAFYRRNLAAGQQGVSVAFDLATHRGYDSDHPRV
Sbjct: 61  GPRATMYAGRPWTVRQYAGFSTAEASNAFYRRNLAAGQQGVSVAFDLATHRGYDSDHPRV 120

Query: 121 QGDVGKAGVAIDSVEDMKILFDGIPLDRISVSMTMNGAVIPILASFIVAGEEQGVSRDKL 180
           QGDVGKAGVAIDSVEDMKILFDGIPLDRISVSMTMNGAVIPILASFIVAGEEQGVSRDKL
Sbjct: 121 QGDVGKAGVAIDSVEDMKILFDGIPLDRISVSMTMNGAVIPILASFIVAGEEQGVSRDKL 180

Query: 181 SGTIQNDILKEFMVRNTYIYPPEPSMRIVADIIEYTAKEMPKFNSISISGYHMQEAGATL 240
           SGTIQNDILKEFMVRNTYIYPPEPSMRIVADIIEYTAKEMPKFNSISISGYHMQEAGATL
Sbjct: 181 SGTIQNDILKEFMVRNTYIYPPEPSMRIVADIIEYTAKEMPKFNSISISGYHMQEAGATL 240

Query: 241 VQELAFTLADGREYVRAALAKGLNVDDFAGRLSFFFAIGMNFFMEAAKLRAARLLWTRIM 300
           VQELAFTLADGREYVRAALAKGLNVDDFAGRLSFFFAIGMNFFMEAAKLRAARLLWTRIM
Sbjct: 241 VQELAFTLADGREYVRAALAKGLNVDDFAGRLSFFFAIGMNFFMEAAKLRAARLLWTRIM 300

Query: 301 QEFKPEKASSLMLRTHCQTSGVSLQEQDPYNNIVRTAFEAMSAVLGGTQSLHTNSFDEAM 360
           QEFKPEKASSLMLRTHCQTSGVSLQEQDPYNNIVRTAFEAMSAVLGGTQSLHTNSFDEAM
Sbjct: 301 QEFKPEKASSLMLRTHCQTSGVSLQEQDPYNNIVRTAFEAMSAVLGGTQSLHTNSFDEAM 360

Query: 361 ALPTDFSARIARNTQLILQHETGVTKVVDPLAGSYYVESLTNELAEKAWGLIEEVEALGG 420
           ALPTDFSARIARNTQLILQHETGVTKVVDPLAGSYYVESLTNELAEKAWGLIEEVEALGG
Sbjct: 361 ALPTDFSARIARNTQLILQHETGVTKVVDPLAGSYYVESLTNELAEKAWGLIEEVEALGG 420

Query: 421 MTKAVNAGLPKRLIEEAATRRQAAVDRAEEVIVGVNKYRLENEQPIDILQIDNAAVRTAQ 480
           MTKAVNAGLPKRLIEEAATRRQAAVDRAEEVIVGVNKYRLENEQPIDILQIDNAAVRTAQ
Sbjct: 421 MTKAVNAGLPKRLIEEAATRRQAAVDRAEEVIVGVNKYRLENEQPIDILQIDNAAVRTAQ 480

Query: 481 VKRIEETRRRRDSQKMKQALDALADVARSGKGNLLAAAVEAARARATVGEITDAMREAFG 540
           VKRIEETRRRRDSQKMKQALDALADVARSGKGNLLAAAVEAARARATVGEITDAMREAFG
Sbjct: 481 VKRIEETRRRRDSQKMKQALDALADVARSGKGNLLAAAVEAARARATVGEITDAMREAFG 540

Query: 541 DYTAIPEVVTDIYGKAYEGDPELGVLAGRLGEATKRLGHKPKIMVAKLGQDGHDRGAKVI 600
           DYTAIPEVVTDIYGKAYEGDPELGVLAGRLGEATKRLGHKPKIMVAKLGQDGHDRGAKVI
Sbjct: 541 DYTAIPEVVTDIYGKAYEGDPELGVLAGRLGEATKRLGHKPKIMVAKLGQDGHDRGAKVI 600

Query: 601 ASAFGDIGFDVVAGPLFQTPEEAADLALAEEVTVIGVSSLAAGHRTLMPQLAEALKKRGG 660
           ASAFGDIGFDVVAGPLFQTPEEAADLALAEEVTVIGVSSLAAGHRTLMPQLAEALKKRGG
Sbjct: 601 ASAFGDIGFDVVAGPLFQTPEEAADLALAEEVTVIGVSSLAAGHRTLMPQLAEALKKRGG 660

Query: 661 EDIIVVCGGVIPRQDYDYLMENGVAAVFGPGTQVLDAARAVLDLIEGKRRNV 712
           EDIIVVCGGVIPRQDYDYLMENGVAAVFGPGTQVLDAARAVLDLIEGKRRNV
Sbjct: 661 EDIIVVCGGVIPRQDYDYLMENGVAAVFGPGTQVLDAARAVLDLIEGKRRNV 712


Lambda     K      H
   0.318    0.134    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1625
Number of extensions: 48
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 712
Length of database: 712
Length adjustment: 39
Effective length of query: 673
Effective length of database: 673
Effective search space:   452929
Effective search space used:   452929
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources