Aligments for a candidate for prpB in Sinorhizobium meliloti 1021

GapMind for catabolism of small carbon sources

Aligments for a candidate for prpB in Sinorhizobium meliloti 1021

Align isocitrate lyase (EC 4.1.3.1) (characterized)
to candidate SMc00768 SMc00768 isocitrate lyase

Query= BRENDA::P9WKK7
         (428 letters)



>FitnessBrowser__Smeli:SMc00768
          Length = 429

 Score =  548 bits (1412), Expect = e-160
 Identities = 277/410 (67%), Positives = 319/410 (77%), Gaps = 4/410 (0%)

Query: 22  RWKDVTRTYSAEDVVALQGSVVEEHTLARRGAEVLWEQLHDLEWVNALGALTGNMAVQQV 81
           R+  + R YSAEDV  L+GSV   ++LA  GA  LW+ +H+ ++VNALGAL+GN A+Q V
Sbjct: 15  RFDGIERPYSAEDVKRLRGSVEIRYSLAEMGANRLWKLIHEEDFVNALGALSGNQAMQMV 74

Query: 82  RAGLKAIYLSGWQVAGDANLSGHTYPDQSLYPANSVPQVVRRINNALQRADQIAKIEGD- 140
           RAGLKAIYLSGWQVA DAN +   YPDQSLYPAN+ P++ +RIN  LQRADQI   EG  
Sbjct: 75  RAGLKAIYLSGWQVAADANTASAMYPDQSLYPANAAPELAKRINRTLQRADQIETAEGKG 134

Query: 141 TSVENWLAPIVADGEAGFGGALNVYELQKALIAAGVAGSHWEDQLASEKKCGHLGGKVLI 200
            SVE W APIVAD EAGFGG LN +E+ KA I AG AG H+EDQLASEKKCGHLGGKVLI
Sbjct: 135 LSVETWFAPIVADAEAGFGGPLNAFEIMKAFIEAGAAGVHYEDQLASEKKCGHLGGKVLI 194

Query: 201 PTQQHIRTLTSARLAADVADVPTVVIARTDAEAATLITSDVDERDQPFITGE--RTREGF 258
           PT  HIR L +ARLAADV   PT+VIARTDAEAA L+TSD+DERD+PF+  +  RT EGF
Sbjct: 195 PTAAHIRNLNAARLAADVMGTPTLVIARTDAEAAKLLTSDIDERDRPFVDYDAGRTVEGF 254

Query: 259 YRTKNGIEPCIARAKAYAPFADLIWMETGTPDLEAARQFSEAVKAEYPDQMLAYNCSPSF 318
           Y+ KNGIEPCIARA AYAP  DLIW ET  PDLE AR+F+E V   +P ++LAYNCSPSF
Sbjct: 255 YQVKNGIEPCIARAIAYAPHCDLIWCETSKPDLEQARKFAEGVHKAHPGKLLAYNCSPSF 314

Query: 319 NWKKHLDDATIAKFQKELAAMGFKFQFITLAGFHALNYSMFDLAYGYAQNQMSAYVELQE 378
           NWKK+LDDATIAKFQ+EL AMG+KFQFITLAGFH LNY MF+LA GY   QM+AY ELQE
Sbjct: 315 NWKKNLDDATIAKFQRELGAMGYKFQFITLAGFHQLNYGMFELARGYRDRQMAAYSELQE 374

Query: 379 REFAAEERGYTATKHQREVGAGYFDRIATTV-DPNSSTTALTGSTEEGQF 427
            EFAAE  GYTATKHQREVG GYFD ++  +    SSTTA+  STE  QF
Sbjct: 375 AEFAAEANGYTATKHQREVGTGYFDAVSVAITGGQSSTTAMKESTEHDQF 424


Lambda     K      H
   0.316    0.130    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 574
Number of extensions: 20
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 428
Length of database: 429
Length adjustment: 32
Effective length of query: 396
Effective length of database: 397
Effective search space:   157212
Effective search space used:   157212
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources