Alignments for a candidate for dctM in Sinorhizobium meliloti 1021

GapMind for catabolism of small carbon sources

Alignments for a candidate for dctM in Sinorhizobium meliloti 1021

Align TRAP C4-dicarboxylate transport system permease, DctM-2 subunit, component of The 2-ketomonocarboxylate transporter (presented in order of affinity - 2-oxovalerate [highest affinity, KD=0.1 μM], 2-oxoisovalerate, 2-oxobutyrate, 2-oxoisocaproate, 2-oxo-3-methylvalerate, pyruvate [lowest affinity, KD=3 μM]) (characterized)
to candidate SMc04249 SMc04249 hypothetical protein

Query= TCDB::D5ATK1
         (611 letters)



>FitnessBrowser__Smeli:SMc04249
          Length = 597

 Score =  841 bits (2172), Expect = 0.0
 Identities = 441/597 (73%), Positives = 504/597 (84%), Gaps = 25/597 (4%)

Query: 2   LMELIAQNMAPIMFVSLIVFLLLGYPVAFSLAANGLVFFIIGVELAPLSGGSINLDWPLL 61
           ++E +A+N+APIMFVSLIVFLLLGYPVAFSLAANGL+FFIIGVELAPLS  SINL WPLL
Sbjct: 1   MIEFVAENLAPIMFVSLIVFLLLGYPVAFSLAANGLLFFIIGVELAPLSD-SINLSWPLL 59

Query: 62  NAMPERFWGVLSNETLLAIPFFTFMGILLEKSGMAEDLLDTIGQLFGPIRGGLAYAVILV 121
           NA+PERFWGV+SN+TLLAIPFFTFMGI+LE+SGMAEDLLDTIGQLFGP+RGGLAYAVI V
Sbjct: 60  NALPERFWGVMSNDTLLAIPFFTFMGIVLERSGMAEDLLDTIGQLFGPVRGGLAYAVIFV 119

Query: 122 GALLAATTGVVAASVIAMGLISLPIMLRYGYDRRIASGVIAASGTLAQIIPPSLVLIVLA 181
           GALLAATTGVVAASVIAMGLISLPIMLRYGYDRRIASGVIAASGTLAQIIPPSLVLIVLA
Sbjct: 120 GALLAATTGVVAASVIAMGLISLPIMLRYGYDRRIASGVIAASGTLAQIIPPSLVLIVLA 179

Query: 182 DQLGRSVGDMYKGALIPGLVLTGLYMLYVLVMSILRPNSMPALPKEARTLGQGVLSFFVA 241
           DQLGRSVGDMY GALIPGLVLTGLYM Y+L+M+ ++ +SMPALP EARTLG GV S  +A
Sbjct: 180 DQLGRSVGDMYAGALIPGLVLTGLYMGYILLMTFVKRHSMPALPLEARTLGSGVASLAIA 239

Query: 242 MGIGIAIFVAAQHWLAGTGAAKNAGILAASIAVIFVYVMALIDKATGLDRMSHLAQQVII 301
           + +  AI  AA  +L+ T   +NA IL A++ +IF+Y+ AL D+   ++ +S LAQQVII
Sbjct: 240 LLVACAIAYAAHVYLSPT-QGENADILGATVGIIFIYLAALADRTLKINALSRLAQQVII 298

Query: 302 VLIPPLALIFLVLGTIFLGIATPTEGGAMGAVGALILSAVKKRLSLEVVREALAATTRLS 361
           VLIPPLALIFLVLGTIFLGIATPTEGGAMGAVGAL+++A K RL++EVVR ALA+TTRLS
Sbjct: 299 VLIPPLALIFLVLGTIFLGIATPTEGGAMGAVGALVMAAGKGRLNMEVVRAALASTTRLS 358

Query: 362 AFVMFILLGARVFSLTFYGVNGHIWVEHLLVSLPGGETGFLIFVSLLVFFLAFFLDFFEL 421
           AFV+FIL+GARVFSLTFYGVNGH+WVEHLL +LPGGE GFLI V++LVFFLAFFLDFFEL
Sbjct: 359 AFVLFILIGARVFSLTFYGVNGHLWVEHLLTALPGGEIGFLIAVNVLVFFLAFFLDFFEL 418

Query: 422 AFIIVPLLVAPAEALGIDLIWFGVILGVNMQTSFMHPPFGFALFFLRSVAPKVPFLDKVT 481
           AFIIVPLL   A+ LGIDLIWFGV+LG+NMQTSFMHPPFGFALF+LRSVA +VP+LD+VT
Sbjct: 419 AFIIVPLLAPAADKLGIDLIWFGVLLGINMQTSFMHPPFGFALFYLRSVAARVPYLDRVT 478

Query: 482 GKLTEPVKTSQIYWGAVPFVCIQIVMIAVVIAFPQLVMHYKGK--PVDVSHVTVTVPG-G 538
           GK+ +PV T QIYWGAVPFVCIQ+VM+A+ + FPQ+V+HYKG    VD S + + VPG G
Sbjct: 479 GKMMQPVTTGQIYWGAVPFVCIQVVMVALTLMFPQMVLHYKGSGAAVDPSTIKIEVPGFG 538

Query: 539 IGGG------LGGGLGMPGGLGLPPIGAQPGAVPAPGGLGGLPPGLGAPAGQPVTPP 589
            GGG       GGGLG+PGGL L      PG  P  GG        G    QP T P
Sbjct: 539 TGGGGLTLPDNGGGLGLPGGLQL------PGGSPLDGG--------GQQPAQPNTAP 581


Lambda     K      H
   0.327    0.146    0.437 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1113
Number of extensions: 45
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 611
Length of database: 597
Length adjustment: 37
Effective length of query: 574
Effective length of database: 560
Effective search space:   321440
Effective search space used:   321440
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources