GapMind for catabolism of small carbon sources


Alignments for a candidate for mcm-small in Sinorhizobium meliloti 1021

Align methylmalonyl-CoA mutase (subunit 1/2) (EC (characterized)
to candidate SM_b20757 SM_b20757 methylmalonyl-CoA mutase

         (155 letters)

          Length = 712

 Score =  117 bits (294), Expect = 3e-31
 Identities = 65/128 (50%), Positives = 82/128 (64%)

           K++VAKLG DGHDRGAKVIA A  D G +VV   L QTPE+    A+ E+  VIG+S L+

             H  LMP++ EALKK G +D+ +V GGVIP +D   L   GV  VF PGT + + A+ V

Query: 142 SKLASTKR 149
             L   KR
Sbjct: 702 LDLIEGKR 709

Lambda     K      H
   0.319    0.140    0.382 

Lambda     K      H
   0.267   0.0410    0.140 

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 243
Number of extensions: 15
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 155
Length of database: 712
Length adjustment: 28
Effective length of query: 127
Effective length of database: 684
Effective search space:    86868
Effective search space used:    86868
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.



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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory