Aligments for a candidate for nbaE in Sinorhizobium meliloti 1021

GapMind for catabolism of small carbon sources

Aligments for a candidate for nbaE in Sinorhizobium meliloti 1021

Align 2-aminomuconic semialdehyde dehydrogenase; Aldehyde dehydrogenase 12; Aldehyde dehydrogenase family 8 member A1; EC 1.2.1.32 (characterized)
to candidate SM_b21301 SM_b21301 ly membrane-anchored aldehyde dehydrogenase

Query= SwissProt::Q9H2A2
         (487 letters)



>FitnessBrowser__Smeli:SM_b21301
          Length = 794

 Score =  315 bits (808), Expect = 3e-90
 Identities = 170/465 (36%), Positives = 269/465 (57%), Gaps = 21/465 (4%)

Query: 10  LENFIDGKFLPCSS--YIDSYDPSTGEVYCRVPNSGKDEIEAAVKAAREAFPSWSSRSPQ 67
           L +FI+G F+  +S    D+++P+TG++  ++ + G+D++ AAV AAR+A   W+  S  
Sbjct: 33  LGHFINGAFVGSASGKSFDTFEPATGKLLAKIAHGGRDDVNAAVAAARKAQGPWAKLSGH 92

Query: 68  ERSRVLNQVADLLEQSLEEFAQAESKDQGKTLALARTMDIPRSVQNFRFFASSSLHHTSE 127
            R+R L  +A L+++     A  E+ D GK +   R +DIP + ++F        +H + 
Sbjct: 93  ARARHLYALARLIQRHARLIAVVEALDNGKPIRETRDIDIPLAARHF--------YHHAG 144

Query: 128 CTQMDHLGCMHYTVRAPVGVAGLISPWNLPLYLLTWKIAPAMAAGNTVIAKPSELTSVTA 187
             Q+       +  + PVGV G + PWN P  +L WK+APA+A GN+VI KP+E T +TA
Sbjct: 145 WAQLQET---EFADQVPVGVVGQVIPWNFPFLMLAWKVAPALALGNSVILKPAEFTPLTA 201

Query: 188 WMLCKLLDKAGVPPGVVNIVFGTGPRVGEALVSHPEVPLISFTGSQPTAERITQLSAPHC 247
            +  +L   AG+PPGV+N+V G G   G  +V H ++  I+FTGS      I + +A   
Sbjct: 202 LLFAELAAAAGLPPGVLNVVTGEG-ETGALIVEHEDIDKIAFTGSTEVGRLIREKTAGSG 260

Query: 248 KKLSLELGGKNPAIIFEDANLDECIPATVRSSFANQGEICLCTSRIFVQKSIYSEFLKRF 307
           K L+LELGGK+P I+F+DA++D  +   V + + NQG++C   SR+ VQ+ +   F  R 
Sbjct: 261 KSLTLELGGKSPFIVFDDADIDAAVEGVVDAIWFNQGQVCCAGSRLLVQEGVAPVFHDRL 320

Query: 308 VEATRKWKVGIPSDPLVSIGALISKAHLEKVRSYVKRALAEGAQIWCGEGVDKLSLPARN 367
                  +VG P D  + + A+++   L+++   V + +AEGA +       K+ LP   
Sbjct: 321 KRRMETLRVGHPLDKAIDMAAIVAPVQLQRIEELVAKGVAEGASMH----QPKIELP--- 373

Query: 368 QAGYFMLPTVITDIKDESCCMTEEIFGPVTCVVPFDSEEEVIERANNVKYGLAATVWSSN 427
           + G F  PT++T ++  S   TEEIFGPV   + F + EE I+ AN+ +YGLAA+VWS  
Sbjct: 374 KGGSFFRPTLLTGVQPTSVVATEEIFGPVAVSMTFRTPEEAIQLANHSRYGLAASVWSET 433

Query: 428 VGRVHRVAKKLQSGLVWTNCWLIRELNLPFGGMKSSGIGREGAKD 472
           +G    VA KL +G+VW N   + + +  FGG + SG GREG ++
Sbjct: 434 IGLALHVAAKLAAGVVWVNATNLFDASSGFGGKRESGFGREGGRE 478



 Score = 91.7 bits (226), Expect = 9e-23
 Identities = 75/245 (30%), Positives = 117/245 (47%), Gaps = 6/245 (2%)

Query: 13  FIDGKFL-PCSSYIDSYDPSTGEVYCRVPNSGKDEIEAAVKAAREAFPSWSSRSPQERSR 71
           F+ GK   P  +Y        G+V   V    + +I  AV AAR A   WSS +   R++
Sbjct: 522 FVGGKQARPDGNYSRPVLSPKGKVIGEVGEGNRKDIRNAVVAARGA-SGWSSATAHNRAQ 580

Query: 72  VLNQVADLLEQSLEEFAQAESKDQGKTLALARTMDIPRSVQNFRFFASSSLHHTSECT-Q 130
           +L  +A+ L     EFA   +   G + A AR  ++  S+   R F+  +     E T  
Sbjct: 581 ILYYIAENLSSRGAEFAGRIAAMTGASAANARA-EVEASIA--RLFSYGAWADKYEGTVH 637

Query: 131 MDHLGCMHYTVRAPVGVAGLISPWNLPLYLLTWKIAPAMAAGNTVIAKPSELTSVTAWML 190
              L  +   +  P GV G+I P   PL  L   +AP +A GN V+A PSE   + A   
Sbjct: 638 QPPLRGVALAMPEPQGVVGVICPPEAPLLGLVSLVAPLIAVGNRVVAVPSEAHPLAATDF 697

Query: 191 CKLLDKAGVPPGVVNIVFGTGPRVGEALVSHPEVPLISFTGSQPTAERITQLSAPHCKKL 250
             +L+ + VPPGV+NIV G+   + +AL +H +V  +   GS   +  + +LS  + K+ 
Sbjct: 698 YSVLETSDVPPGVINIVTGSAIELAKALAAHNDVDALWAFGSPELSTLVEKLSVGNLKRT 757

Query: 251 SLELG 255
            ++ G
Sbjct: 758 FVDYG 762


Lambda     K      H
   0.319    0.133    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 869
Number of extensions: 35
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 487
Length of database: 794
Length adjustment: 37
Effective length of query: 450
Effective length of database: 757
Effective search space:   340650
Effective search space used:   340650
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources