GapMind for catabolism of small carbon sources

 

L-tyrosine catabolism in Sinorhizobium meliloti 1021

Best path

aroP, HPD, hmgA, maiA, fahA, aacS, atoB

Also see fitness data for the top candidates

Rules

Overview: Tyrosine utilization in GapMind is based on MetaCyc pathway tyrosine degradation I, via homogentisate (link). This pathway requires oxygen. Another pathway via 4-hydroxyphenylacetate is known (link), but the 4-hydroxyphenylpyruvate oxidase has not been linked to sequence. The other MetaCyc pathways do not yield fixed carbon or are not reported in prokaryotes.

19 steps (11 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
aroP L-tyrosine transporter (AroP/FywP)
HPD 4-hydroxyphenylpyruvate dioxygenase SMc03211 SM_b20581
hmgA homogentisate dioxygenase SMc03208
maiA maleylacetoacetate isomerase SMc03206 SM_b20005
fahA fumarylacetoacetate hydrolase SMc03207 SM_b21112
aacS acetoacetyl-CoA synthetase SMc00774 SMc02162
atoB acetyl-CoA C-acetyltransferase SMc03879 SMa1450
Alternative steps:
Ac3H11_1692 L-tyrosine ABC transporter, ATPase component 2 SMc02358 SMc01948
Ac3H11_1693 L-tyrosine ABC transporter, ATPase component 1 SMc01949 SMc02357
Ac3H11_1694 L-tyrosine ABC transporter, permease component 2 SMc01950
Ac3H11_1695 L-tyrosine ABC transporter, permease component 1 SMc01951 SMc02359
Ac3H11_2396 L-tyrosine ABC transporter, substrate-binding component component SMc01946 SMc00078
atoA acetoacetyl-CoA transferase, A subunit
atoD acetoacetyl-CoA transferase, B subunit
CAT L-tyrosine transporter CAT
MCT10 L-tyrosine transporter MCT10
TAT1 L-tyrosine permease TAT1
tyrP Tyrosine permease
tyt1 L-tyrosine:Na+ symporter Tyt1

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory