GapMind for catabolism of small carbon sources

 

Aligments for a candidate for bch in Sinorhizobium meliloti 1021

Align 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial; 3-hydroxyisobutyryl-coenzyme A hydrolase; HIB-CoA hydrolase; HIBYL-CoA-H; EC 3.1.2.4 (characterized)
to candidate SM_b20752 SM_b20752 3-hydroxyisobutyryl-CoA hydrolase

Query= SwissProt::Q5XIE6
         (385 letters)



>lcl|FitnessBrowser__Smeli:SM_b20752 SM_b20752
           3-hydroxyisobutyryl-CoA hydrolase
          Length = 356

 Score =  256 bits (654), Expect = 7e-73
 Identities = 145/355 (40%), Positives = 211/355 (59%), Gaps = 8/355 (2%)

Query: 28  MSKHTETAEVLLERRGCAGVITLNRPKLLNALSLNMIRQIYPQLKKWERDPDTFLIIIKG 87
           M   T   EV++ER+G  G + LNRP+ LN+L+  MIR I   L ++ER+P+   +++ G
Sbjct: 1   MEMQTTLPEVIVERQGAIGRLRLNRPRALNSLNRTMIRAIAAALTEFERNPEIAAVLVTG 60

Query: 88  AGGKAFCAGGDIKALSEAKKAGQTLSQDLFREEYILNNAIASCQKPYVALIDGITMGGGV 147
            G +  CAGGDI+ + E+ +         +REE+I+N+ I++  KPY+A++DGI MGGGV
Sbjct: 61  EGERGLCAGGDIRMIYESGRERPGEGAQFWREEFIVNSRISAYSKPYIAIMDGIVMGGGV 120

Query: 148 GLSVHGQFRVATERSLFAMPETGIGLFPDVGGGYFLPRLQGKLGYFLALTGFRLKGRDVH 207
           G+S HG  RV TER+ FAMPETGIG F DVG  + LPR  G+ G +L LTG  +    V 
Sbjct: 121 GVSSHGSHRVVTERTRFAMPETGIGYFTDVGATWLLPRAPGEFGTYLGLTGRDIGAAAVI 180

Query: 208 RAGIATHFVDSEKLHVLEEELLALK-SPSAEDVAGVLESYHAKSKMGQDKSIIFEEHMDK 266
            A +A  FV SE +  L   L +L  S +A+DV+  +     ++   +  +    +H+  
Sbjct: 181 HARLADSFVPSEMIGELLGALSSLSGSATADDVSAAI-----RAVSSEPPASALLDHLSV 235

Query: 267 INSCFSANTVEQILENLRQDGSPFAMEQIKVINKMSPTSLKITLRQLMEG-STKTLQEVL 325
           I+ CF+ N VE+I   L +D S FA E ++++   S  SLK+TL  L EG S+ TL E L
Sbjct: 236 IDRCFAFNAVEEIFAALEKDESDFARETLELLKTRSAISLKLTLSLLREGRSSATLNECL 295

Query: 326 TMEYRLTQACMEGHDFHEGVRAVLIDKDQTPKWKPADLKDVTDEDLNSYFKSLGS 380
             EY  T   +   DF+EGVRA +IDKD+ PKW    L + T E L  + ++ G+
Sbjct: 296 EREYAATLGMLSNPDFYEGVRAAVIDKDRNPKWS-VGLSEATPELLARFGRNDGA 349


Lambda     K      H
   0.320    0.136    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 299
Number of extensions: 9
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 385
Length of database: 356
Length adjustment: 30
Effective length of query: 355
Effective length of database: 326
Effective search space:   115730
Effective search space used:   115730
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory