Alignments for a candidate for mmsA in Sinorhizobium meliloti 1021

GapMind for catabolism of small carbon sources

Alignments for a candidate for mmsA in Sinorhizobium meliloti 1021

Align Methylmalonate-semialdehyde dehydrogenase [inositol] (EC 1.2.1.27) (characterized)
to candidate SM_b21301 SM_b21301 ly membrane-anchored aldehyde dehydrogenase

Query= reanno::Caulo:CCNA_01360
         (500 letters)



>FitnessBrowser__Smeli:SM_b21301
          Length = 794

 Score =  231 bits (588), Expect = 9e-65
 Identities = 152/472 (32%), Positives = 241/472 (51%), Gaps = 20/472 (4%)

Query: 7   HFIGGQKVDGASGRFGEVFDPNTGKVQARVALASAGELNTAIANAKVAQAAWAATNPQRR 66
           HFI G  V  ASG+  + F+P TGK+ A++A     ++N A+A A+ AQ  WA  +   R
Sbjct: 35  HFINGAFVGSASGKSFDTFEPATGKLLAKIAHGGRDDVNAAVAAARKAQGPWAKLSGHAR 94

Query: 67  ARVMFEFKRLLEVHMDELAALLSSEHGKVIADSKG-DIQRGLEVIEFACGVPHLLKGEYT 125
           AR ++   RL++ H   +A + + ++GK I +++  DI           G   L + E+ 
Sbjct: 95  ARHLYALARLIQRHARLIAVVEALDNGKPIRETRDIDIPLAARHFYHHAGWAQLQETEFA 154

Query: 126 QGAGPGIDVYSMRQPLGVVAGITPFNFPAMIPMWMFGPAIATGNAFILKPSERDPSVPVR 185
                       + P+GVV  + P+NFP ++  W   PA+A GN+ ILKP+E  P   + 
Sbjct: 155 D-----------QVPVGVVGQVIPWNFPFLMLAWKVAPALALGNSVILKPAEFTPLTALL 203

Query: 186 LAELMIEAGLPPGVLNVVHGDKDCVEAILDHPDIKAVSFVGSSDIAQSVFQRAGAAGKRV 245
            AEL   AGLPPGVLNVV G+ +    I++H DI  ++F GS+++ + + ++   +GK +
Sbjct: 204 FAELAAAAGLPPGVLNVVTGEGETGALIVEHEDIDKIAFTGSTEVGRLIREKTAGSGKSL 263

Query: 246 QAMGGAKNHGLVMPDADLDQAVADIIGAAYGSAGERCMALPVVVPVGEKTATALREKLVA 305
               G K+  +V  DAD+D AV  ++ A + + G+ C A   ++ V E  A    ++L  
Sbjct: 264 TLELGGKSPFIVFDDADIDAAVEGVVDAIWFNQGQVCCAGSRLL-VQEGVAPVFHDRLKR 322

Query: 306 AIGGLRVGVSTDPDAHYGPVVSAAHKARIESYIQMGVDEGAELVVDGRGFSLQGHEEGFF 365
            +  LRVG   D       +V+     RIE  +  GV EGA +        ++  + G F
Sbjct: 323 RMETLRVGHPLDKAIDMAAIVAPVQLQRIEELVAKGVAEGASM----HQPKIELPKGGSF 378

Query: 366 VGPTLFDHVKPTSRSYHDEIFGPVLQMVRAESLEEGIALASRHQYGNGVAIFTRNGDAAR 425
             PTL   V+PTS    +EIFGPV   +   + EE I LA+  +YG   ++++     A 
Sbjct: 379 FRPTLLTGVQPTSVVATEEIFGPVAVSMTFRTPEEAIQLANHSRYGLAASVWSETIGLAL 438

Query: 426 EFADQVEVGMVGINVPIPVPVAYHSFGGWKRSGFGDLNQYGMDGVRFYTRTK 477
             A ++  G+V +N    +  A   FGG + SGFG   + G +G   Y + K
Sbjct: 439 HVAAKLAAGVVWVNA-TNLFDASSGFGGKRESGFG--REGGREGCYEYLKPK 487



 Score = 71.2 bits (173), Expect = 1e-16
 Identities = 54/228 (23%), Positives = 103/228 (45%), Gaps = 4/228 (1%)

Query: 8   FIGGQKVDGASGRFGEVFDPNTGKVQARVALASAGELNTAIANAKVAQAAWAATNPQRRA 67
           F+GG++          V  P  GKV   V   +  ++  A+  A+ A + W++     RA
Sbjct: 522 FVGGKQARPDGNYSRPVLSPK-GKVIGEVGEGNRKDIRNAVVAARGA-SGWSSATAHNRA 579

Query: 68  RVMFEFKRLLEVHMDELAALLSSEHGKVIADSKGDIQRGLEVIEFACGVPHLLKGEYTQG 127
           ++++     L     E A  +++  G   A+++ +++  +  +          +G   Q 
Sbjct: 580 QILYYIAENLSSRGAEFAGRIAAMTGASAANARAEVEASIARLFSYGAWADKYEGTVHQP 639

Query: 128 AGPGIDVYSMRQPLGVVAGITPFNFPAMIPMWMFGPAIATGNAFILKPSERDPSVPVRLA 187
              G+ + +M +P GVV  I P   P +  + +  P IA GN  +  PSE  P       
Sbjct: 640 PLRGVAL-AMPEPQGVVGVICPPEAPLLGLVSLVAPLIAVGNRVVAVPSEAHPLAATDFY 698

Query: 188 ELMIEAGLPPGVLNVVHGDK-DCVEAILDHPDIKAVSFVGSSDIAQSV 234
            ++  + +PPGV+N+V G   +  +A+  H D+ A+   GS +++  V
Sbjct: 699 SVLETSDVPPGVINIVTGSAIELAKALAAHNDVDALWAFGSPELSTLV 746


Lambda     K      H
   0.320    0.137    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1032
Number of extensions: 61
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 500
Length of database: 794
Length adjustment: 38
Effective length of query: 462
Effective length of database: 756
Effective search space:   349272
Effective search space used:   349272
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources