Align Malate synthase G (EC 2.3.3.9) (characterized)
to candidate SMc02581 SMc02581 malate synthase G
Query= reanno::psRCH2:GFF353 (726 letters) >FitnessBrowser__Smeli:SMc02581 Length = 723 Score = 982 bits (2539), Expect = 0.0 Identities = 473/723 (65%), Positives = 580/723 (80%), Gaps = 2/723 (0%) Query: 3 ERVQVGGLQVAKVLYDFVNNEAIPGTGVDAAAFWAGADSVIHDLAPKNRALLAKRDDLQA 62 +RV+ GLQ+ L+ F+ EA+PGTGVDA F++ ++HDL PKNRALL KRD+LQA Sbjct: 2 DRVEKYGLQIDAGLHRFLVEEAMPGTGVDADRFFSAFSDLVHDLGPKNRALLVKRDELQA 61 Query: 63 QIDAWHQARAGQAHDAVAYKSFLQEIGYLLPEAEDFQATTENVDEEIARMAGPQLVVPIM 122 ++D W++ G D AY++FL+EIGYLLPE DF +T NVD EIA +AGPQLVVP+M Sbjct: 62 RLDGWYREH-GAPVDMEAYEAFLREIGYLLPEGPDFYVSTANVDSEIATIAGPQLVVPVM 120 Query: 123 NARFALNAANARWGSLYDALYGTDAISEADGASKGPGYNEIRGNKVIAYARNFLNEAAPL 182 NAR+ALNAANARWGSLYDALYGTDAI+E DGA +G GYN RG KVIA+AR FL+ +APL Sbjct: 121 NARYALNAANARWGSLYDALYGTDAIAETDGAERGKGYNPKRGAKVIAWAREFLDASAPL 180 Query: 183 ETGSHVDSTGYRIEGGKLVVSLKDGSTTGLKNPAQLQGFQGEASAPIAVLLKNNGIHFEI 242 G D+ + +EG L V+L DG+ + +N Q G+ G+ +AP ++L+ NG+H I Sbjct: 181 AAGRWSDAKSFSVEGATLTVTLADGTKSAPRNSVQFAGYAGDPAAPSEIVLRRNGLHIVI 240 Query: 243 QIDPASPIGQTDAAGVKDILMESALTTIMDCEDSIAAVDADDKTVVYRNWLGLMKGDLVE 302 +D +PIG+ DAAG+ D+++ESA+TTIMDCEDSIAAVDA+DK +VYRNWLGLMKGDL E Sbjct: 241 VLDATTPIGKADAAGISDVVLESAITTIMDCEDSIAAVDAEDKVLVYRNWLGLMKGDLEE 300 Query: 303 ELEKGGKRITRAMNPDRVYTKADGNGELTLHGRSLLFIRNVGHLMTNDAILDKEGNEVPE 362 E+ KGG+ TR +NPDR YT DG LTL GRSL+ +RNVGHLMTN A+LD++G EVPE Sbjct: 301 EVTKGGRAFTRRLNPDRAYTAPDG-ATLTLPGRSLMLVRNVGHLMTNPAVLDRDGEEVPE 359 Query: 363 GIMDGLFTSLIAVHNLNGNTSRKNTRTGSMYIVKPKMHGPEEVAFATELFGRVEDVLGLP 422 G+MD + T+LIA+H++ N R N+R+GSMY+VKPKMHGPEEVAFA E+F RVE LGLP Sbjct: 360 GLMDAMVTALIALHDIGRNGRRANSRSGSMYVVKPKMHGPEEVAFACEIFARVEAALGLP 419 Query: 423 RNTLKVGIMDEERRTTINLKACIKEARERVVFINTGFLDRTGDEIHTSMEAGPMVRKAAM 482 N +K+GIMDEERRTT+NLK CI+ ARERVVFINTGFLDRTGDEIHTSMEAGPM+RK M Sbjct: 420 ANAMKMGIMDEERRTTVNLKECIRAARERVVFINTGFLDRTGDEIHTSMEAGPMIRKGDM 479 Query: 483 KAEKWISAYENNNVDVGLACGLQGKAQIGKGMWAMPDLMAAMLEQKVGHPMAGANTAWVP 542 K WISAYEN NVDVGL CGL G AQIGKGMWAMPDLMAAMLEQK+ HP AGANTAWVP Sbjct: 480 KQAPWISAYENWNVDVGLECGLSGHAQIGKGMWAMPDLMAAMLEQKIVHPKAGANTAWVP 539 Query: 543 SPTAATLHAMHYHKIDVQARQVELAKREKASIDDILTIPLAQDTNWSEEEKRNELDNNSQ 602 SPTAATLHA HYH+++V Q L R +A + DIL++P+A NW+EEE + ELDNN+Q Sbjct: 540 SPTAATLHATHYHRVNVAEVQASLKSRTRAKLADILSVPVAARPNWTEEEIQRELDNNAQ 599 Query: 603 GILGYMVRWVEQGVGCSKVPDINDIALMEDRATLRISSQHVANWMRHGVVTKDQVVESLK 662 GILGY+VRWV+QGVGCSKVPDIN++ LMEDRATLRIS+QH+ANW+ HG+V++ Q+VE+++ Sbjct: 600 GILGYVVRWVDQGVGCSKVPDINNVGLMEDRATLRISAQHMANWLHHGIVSEAQIVETMQ 659 Query: 663 RMAPVVDRQNQGDPLYRPMAPDFDNSVAFQAALELVLEGTKQPNGYTEPVLHRRRREFKA 722 RMA +VD QN GDP Y+PMA F+ S+AFQAAL+LVL+G +QPNGYTEPVLHRRR E KA Sbjct: 660 RMAAIVDGQNAGDPNYQPMAGRFEESIAFQAALDLVLKGREQPNGYTEPVLHRRRLELKA 719 Query: 723 KNG 725 K G Sbjct: 720 KQG 722 Lambda K H 0.316 0.133 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1481 Number of extensions: 59 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 726 Length of database: 723 Length adjustment: 40 Effective length of query: 686 Effective length of database: 683 Effective search space: 468538 Effective search space used: 468538 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 55 (25.8 bits)
Align candidate SMc02581 SMc02581 (malate synthase G)
to HMM TIGR01345 (glcB: malate synthase G (EC 2.3.3.9))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01345.hmm # target sequence database: /tmp/gapView.4896.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01345 [M=721] Accession: TIGR01345 Description: malate_syn_G: malate synthase G Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 0 1177.3 0.0 0 1177.1 0.0 1.0 1 lcl|FitnessBrowser__Smeli:SMc02581 SMc02581 malate synthase G Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Smeli:SMc02581 SMc02581 malate synthase G # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 1177.1 0.0 0 0 2 720 .. 3 720 .. 2 721 .. 0.99 Alignments for each domain: == domain 1 score: 1177.1 bits; conditional E-value: 0 TIGR01345 2 rvdagrlqvakklkdfveeevlpgtgvdaekfwsgfdeivrdlapenrellakrdeiqaaideyhrknkgvidke 76 rv++ +lq+++ l++f+ ee++pgtgvda+ f+s+f+++v+dl p+nr ll krde+qa +d ++r+ +++d e lcl|FitnessBrowser__Smeli:SMc02581 3 RVEKYGLQIDAGLHRFLVEEAMPGTGVDADRFFSAFSDLVHDLGPKNRALLVKRDELQARLDGWYREHGAPVDME 77 577889********************************************************************* PP TIGR01345 77 ayksflkeigylveepervtietenvdseiasqagpqlvvpvlnaryalnaanarwgslydalygsnvipeedga 151 ay+ fl+eigyl +e + t nvdseia+ agpqlvvpv+naryalnaanarwgslydalyg+++i+e+dga lcl|FitnessBrowser__Smeli:SMc02581 78 AYEAFLREIGYLLPEGPDFYVSTANVDSEIATIAGPQLVVPVMNARYALNAANARWGSLYDALYGTDAIAETDGA 152 *************************************************************************** PP TIGR01345 152 ekgkeynpkrgekviefarefldeslplesgsyadvvkykivdkklavqlesgkvtrlkdeeqfvgyrgdaadpe 226 e+gk ynpkrg kvi++arefld s pl +g + d+ ++++ l+v+l +g + ++ qf gy gd a+p+ lcl|FitnessBrowser__Smeli:SMc02581 153 ERGKGYNPKRGAKVIAWAREFLDASAPLAAGRWSDAKSFSVEGATLTVTLADGTKSAPRNSVQFAGYAGDPAAPS 227 *************************************************************************** PP TIGR01345 227 villktnglhielqidarhpigkadkakvkdivlesaittildcedsvaavdaedkvlvyrnllglmkgtlkekl 301 i+l++nglhi + +da+ pigkad a++ d+vlesaitti+dceds+aavdaedkvlvyrn+lglmkg+l+e++ lcl|FitnessBrowser__Smeli:SMc02581 228 EIVLRRNGLHIVIVLDATTPIGKADAAGISDVVLESAITTIMDCEDSIAAVDAEDKVLVYRNWLGLMKGDLEEEV 302 *************************************************************************** PP TIGR01345 302 ekngriikrklnedrsytaangeelslhgrsllfvrnvghlmtipviltdegeeipegildgvltsvialydlkv 376 +k gr ++r+ln dr+yta++g +l+l+grsl++vrnvghlmt+p++l+ +gee+peg++d+++t++ial+d+ + lcl|FitnessBrowser__Smeli:SMc02581 303 TKGGRAFTRRLNPDRAYTAPDGATLTLPGRSLMLVRNVGHLMTNPAVLDRDGEEVPEGLMDAMVTALIALHDIGR 377 *************************************************************************** PP TIGR01345 377 qnklrnsrkgsvyivkpkmhgpeevafanklftriedllglerhtlkvgvmdeerrtslnlkaciakvkervafi 451 +++ nsr gs+y+vkpkmhgpeevafa ++f+r+e lgl+ +++k+g+mdeerrt++nlk ci ++erv+fi lcl|FitnessBrowser__Smeli:SMc02581 378 NGRRANSRSGSMYVVKPKMHGPEEVAFACEIFARVEAALGLPANAMKMGIMDEERRTTVNLKECIRAARERVVFI 452 *************************************************************************** PP TIGR01345 452 ntgfldrtgdeihtsmeagamvrkadmksapwlkayernnvaagltcglrgkaqigkgmwampdlmaemlekkgd 526 ntgfldrtgdeihtsmeag+m+rk+dmk apw++aye+ nv+ gl cgl g+aqigkgmwampdlma mle+k+ lcl|FitnessBrowser__Smeli:SMc02581 453 NTGFLDRTGDEIHTSMEAGPMIRKGDMKQAPWISAYENWNVDVGLECGLSGHAQIGKGMWAMPDLMAAMLEQKIV 527 *************************************************************************** PP TIGR01345 527 qlragantawvpsptaatlhalhyhrvdvqkvqkeladaerraelkeiltipvaentnwseeeikeeldnnvqgi 601 ++agantawvpsptaatlha+hyhrv+v +vq++l++ +ra+l +il +pva nw+eeei++eldnn+qgi lcl|FitnessBrowser__Smeli:SMc02581 528 HPKAGANTAWVPSPTAATLHATHYHRVNVAEVQASLKSR-TRAKLADILSVPVAARPNWTEEEIQRELDNNAQGI 601 ************************************998.899******************************** PP TIGR01345 602 lgyvvrwveqgigcskvpdihnvalmedratlrissqhlanwlrhgivskeqvleslermakvvdkqnagdeayr 676 lgyvvrwv+qg+gcskvpdi nv lmedratlris+qh+anwl hgivs q++e+++rma +vd qnagd+ y+ lcl|FitnessBrowser__Smeli:SMc02581 602 LGYVVRWVDQGVGCSKVPDINNVGLMEDRATLRISAQHMANWLHHGIVSEAQIVETMQRMAAIVDGQNAGDPNYQ 676 *************************************************************************** PP TIGR01345 677 pmadnleasvafkaakdlilkgtkqpsgytepilharrlefkek 720 pma+ +e+s+af+aa dl+lkg +qp+gytep+lh+rrle k+k lcl|FitnessBrowser__Smeli:SMc02581 677 PMAGRFEESIAFQAALDLVLKGREQPNGYTEPVLHRRRLELKAK 720 ******************************************98 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (721 nodes) Target sequences: 1 (723 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.03u 0.01s 00:00:00.04 Elapsed: 00:00:00.04 # Mc/sec: 12.49 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory