GapMind for catabolism of small carbon sources

 

Alignments for a candidate for adiA in Synechococcus elongatus PCC 7942

Align Arginine decarboxylase proenzyme; ADC; ArgDC; EC 4.1.1.19; Pyruvoyl-dependent arginine decarboxylase (uncharacterized)
to candidate Synpcc7942_2249 Synpcc7942_2249 S-adenosylmethionine decarboxylase proenzyme

Query= curated2:Q9YG22
         (132 letters)



>FitnessBrowser__SynE:Synpcc7942_2249
          Length = 141

 Score = 87.4 bits (215), Expect = 7e-23
 Identities = 44/119 (36%), Positives = 64/119 (53%), Gaps = 2/119 (1%)

Query: 9   VGKHVYGSLYGVPREKATDEEYLRGVVVRAAESAGATVHAVNSWTIPGEKGGVSVIVLVL 68
           VG H    LYG P E   D + ++  +  AA  AGAT+          E  GV+ + L+ 
Sbjct: 17  VGTHCILELYGCPAELLNDADQIQANLRAAATEAGATLLQETCHRF--EPQGVTALALLA 74

Query: 69  ESHLALHTWPEYDYATFDIYTCGEHTDPWKAFELLLSELKPRKYTVHYVDRSQEKTVLE 127
           ESH+++HTWPE  YA  D++TCG HT P  A   L+   + R+YT+H + R    ++ E
Sbjct: 75  ESHISIHTWPESGYAAVDVFTCGSHTQPETACHFLIEAFRSRQYTLHTLRRQPPASIRE 133


Lambda     K      H
   0.317    0.134    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 82
Number of extensions: 3
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 132
Length of database: 141
Length adjustment: 15
Effective length of query: 117
Effective length of database: 126
Effective search space:    14742
Effective search space used:    14742
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 42 (20.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory