Align Carbon-nitrogen hydrolase family protein; EC 3.5.-.- (characterized, see rationale)
to candidate Synpcc7942_2145 Synpcc7942_2145 hypothetical protein
Query= uniprot:Q5NHL7_FRATT (286 letters) >lcl|FitnessBrowser__SynE:Synpcc7942_2145 Synpcc7942_2145 hypothetical protein Length = 295 Score = 223 bits (567), Expect = 5e-63 Identities = 120/292 (41%), Positives = 178/292 (60%), Gaps = 8/292 (2%) Query: 2 ANIKVAVVQLSFNDNEAENLAKLESKIIQAAKNGAKIILTPELPSYLYFCKKQNSKYFDL 61 + ++VA+ QL+F+D + ++ + I +AA GA++I+ PE+ YFC+ ++ FD Sbjct: 3 STLRVALAQLAFSDQPVIDRDRVTAAIREAAAAGAELIVLPEIHGGYYFCQTEDPAQFDR 62 Query: 62 AKTIDESPIVKLYKLLAHKYNIVLPASFFERDGNACY-NSIAMIDADGSIMGVYRKAHIP 120 A++I P Y +A + ++VL S FER Y N+ +I+ DG+I G YRK HIP Sbjct: 63 AESIP-GPSTDYYSAIARELSVVLILSLFERRAAGLYHNTAVVIERDGTIAGRYRKMHIP 121 Query: 121 DGIGYQEKYYFSPGSAGFKVWDTKYAKVGVGICWDQWFPEAARVMALKGAEILLYPTAIG 180 D Y EK+YF+PG GF+ T K+GV +CWDQW+PEAAR+MAL GAE+L+YPTAIG Sbjct: 122 DDPAYYEKFYFTPGDLGFEPIQTSVGKLGVLVCWDQWYPEAARLMALAGAELLIYPTAIG 181 Query: 181 SEPH-LPDYDSK--DHWQRVMQGHAAANMLPVLASNRYATEANDDITAT---YYGSSFIT 234 +P +P+ + + WQ V +GHA AN +PVL+ NR E + D A ++GSSFI Sbjct: 182 WDPQDVPEEQQRQLEAWQTVQRGHAIANGIPVLSVNRVGFEPSPDPAAAGSQFWGSSFIA 241 Query: 235 DHTGDKIAEADRSGDDILYATFDFAELQQQRFYWGLFRDRRPELYDEIVRKY 286 G+ +A+A ++L A D +Q R W RDRR + Y ++VR+Y Sbjct: 242 GPQGEWLAKAGDREPELLIADLDRDRSEQVRRIWPFLRDRRIDAYGDLVRRY 293 Lambda K H 0.320 0.136 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 278 Number of extensions: 19 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 286 Length of database: 295 Length adjustment: 26 Effective length of query: 260 Effective length of database: 269 Effective search space: 69940 Effective search space used: 69940 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory