GapMind for catabolism of small carbon sources

 

Aligments for a candidate for davT in Synechococcus elongatus PCC 7942

Align 5-aminovalerate transaminase (EC 2.6.1.48) (characterized)
to candidate Synpcc7942_0031 Synpcc7942_0031 aminotransferase

Query= reanno::pseudo6_N2E2:Pf6N2E2_4013
         (425 letters)



>lcl|FitnessBrowser__SynE:Synpcc7942_0031 Synpcc7942_0031
           aminotransferase
          Length = 424

 Score =  145 bits (365), Expect = 3e-39
 Identities = 123/416 (29%), Positives = 195/416 (46%), Gaps = 26/416 (6%)

Query: 25  PIFAESAKNATVTDVEGREFIDFAGGIAVLNTGHVHPKIIAAVTEQLNKLTHTCFQVLAY 84
           P+   S K A +T  +GRE ID      V   GH H +I+ A+ +Q   L H  F   ++
Sbjct: 20  PLKVVSGKGARLTLADGRELIDCISSWWVNLHGHAHLRIVEAIAQQAATLEHVIFAGFSH 79

Query: 85  EPYVELCEKINAKVPGDFAKKTLLVTTGSEAVENAVKIA-----RAATGRAGVIAFTGAY 139
           EP   L  ++  K+  +   +      GS AVE A+K+A          R+ ++AF GAY
Sbjct: 80  EPAERLAMEL-CKILPEKLTRVFFSDNGSTAVEVALKMALQYWHNLDQPRSRILAFDGAY 138

Query: 140 HGRTMMTLGLTGKVVPYSAGMGLMPGGIFRALYPNELHG-VSIDDSIASIERIFKNDAEP 198
           HG T   + + G+   ++A    +   +    YP    G  +++   A+     +     
Sbjct: 139 HGDTFGAMSV-GERSLFNAPFEKLLFSVEFLPYPETWWGDETVEAKEAAAIAAVEQALAA 197

Query: 199 RDIAAIIIEP-VQGEGGFYVAPKEFMKRLRALCDQHGILLIADEVQTGAGRTGTFFAMEQ 257
            D+AA+IIEP VQG GG  +A  +F+++L A     G LLIADEV TG GRTG +FA ++
Sbjct: 198 GDVAAVIIEPLVQGAGGMRMARPQFLQQLAARVQAAGSLLIADEVMTGFGRTGAWFACQR 257

Query: 258 MGVAADLTTFAKSIAGGF-PLAGVCGKAEYMDAIAPGG------LGGTYAGSPIACAAAL 310
            G+  DL   +K + GGF PL+         D    G        G +Y  +P+ CAAA+
Sbjct: 258 AGIQPDLICLSKGLTGGFLPLSITVATEVIYDTFCSGNPDHTFYHGHSYTANPLGCAAAI 317

Query: 311 AVMEVFEEEHLLDRCKAVGERLVAGLKAIQKKYPVIGDVRALGAMIAVELF-ENGDSHKP 369
           A +E+  +   +   + + +  + GL+ +  ++P +   R  G + A +L  + G    P
Sbjct: 318 ASLELLLDSEAI--VQGLEDAHLPGLELL-AQHPKVTRPRLTGGIAACDLVSDRGGYLDP 374

Query: 370 NAAAVAQVVAKARDKGLILLSCGTYGNVLRVLVPLTAPDEQLDKGLAILEECFSEL 425
               V Q    A  +GL+L      GNVL +L P      +L    A + +   E+
Sbjct: 375 IGLRVRQA---AIARGLLL---RPLGNVLYLLPPYCLTPTELQDIYAAIADLLDEI 424


Lambda     K      H
   0.320    0.137    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 464
Number of extensions: 18
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 425
Length of database: 424
Length adjustment: 32
Effective length of query: 393
Effective length of database: 392
Effective search space:   154056
Effective search space used:   154056
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory