GapMind for catabolism of small carbon sources

 

Alignments for a candidate for gltL in Synechococcus elongatus PCC 7942

Align Amino acid ABC transporter ATP binding protein, component of Amino acid transporter, AatJMQP. Probably transports L-glutamic acid, D-glutamine acid, L-glutamine and N-acetyl L-glutamic acid (Johnson et al. 2008). Very similar to 3.A.1.3.19 of P. putida (characterized)
to candidate Synpcc7942_1414 Synpcc7942_1414 ATPase

Query= TCDB::Q9I405
         (244 letters)



>FitnessBrowser__SynE:Synpcc7942_1414
          Length = 241

 Score =  149 bits (377), Expect = 4e-41
 Identities = 93/225 (41%), Positives = 130/225 (57%), Gaps = 8/225 (3%)

Query: 1   MISIKNVSKWYGD----FQVLTDCSTEVAKGEVVVVCGPSGSGKSTLIKCVNALEPFQKG 56
           +I  ++VSK YG+     + L     +V  GE   + G SGSGKST +  +  L+    G
Sbjct: 8   VIQFEHVSKIYGEGETTVRALDHVDFQVRAGEYCAIMGASGSGKSTAMNLIGCLDRPTAG 67

Query: 57  DIVVDGTSIADPKTN-LPKLRSR-VGMVFQHFELFPHLSITENLTIAQIKVLGRSKEEAT 114
              +DGT +AD   + L  +R+R +G VFQ F L P LS  EN+ +  I   G S++E  
Sbjct: 68  RYYLDGTDVADLDDDALAAVRNRKIGFVFQQFHLLPQLSAVENVMLPMIYA-GISQQERR 126

Query: 115 KKGLALLERVGLKEHAHKHPGQLSGGQQQRVAIARALAMDPVVMLFDEPTSALDPEMVNE 174
            + +A L +VGL +     P QLSGGQQQRVAIARA+   PV++L DEPT ALD +   E
Sbjct: 127 DRAVAALTQVGLAQRLDNKPNQLSGGQQQRVAIARAIVNQPVLLLADEPTGALDSQTTEE 186

Query: 175 VLDVMVQLAHEGMTMMCVTHEMGFARKVANRVIFMDRGQIVEDCE 219
           VL++  QL   G+T++ VTHE   A + A RVI+   GQI  + +
Sbjct: 187 VLNIFDQLHQRGITIVIVTHEAEVADR-AERVIWFRDGQIQRETQ 230


Lambda     K      H
   0.320    0.135    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 152
Number of extensions: 7
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 244
Length of database: 241
Length adjustment: 23
Effective length of query: 221
Effective length of database: 218
Effective search space:    48178
Effective search space used:    48178
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory