Align High-affinity branched-chain amino acid transport ATP-binding protein (characterized, see rationale)
to candidate Synpcc7942_0513 Synpcc7942_0513 ATPase
Query= uniprot:A0A159ZWL6 (233 letters) >FitnessBrowser__SynE:Synpcc7942_0513 Length = 250 Score = 130 bits (328), Expect = 2e-35 Identities = 83/235 (35%), Positives = 126/235 (53%), Gaps = 4/235 (1%) Query: 2 LQFENVSTFYGKIQALHSVNVEVRQGEIVTLIGANGAGKSTLLMTLCGSPQAHSGSIRYM 61 ++ ENV YGK ++ V++ V QGE+V L+G NGAGK+T G + G + Sbjct: 11 IRLENVRKSYGKRLIVNRVSLSVAQGEVVGLLGPNGAGKTTTFYMTTGLERPDEGHVWLD 70 Query: 62 GEELVGQDSSHIMRKSIAVVPEGRRVFARLTVEENLAMGGFFTD-KGDYQEQMDKVLHLF 120 EL + R I + + +F LTV ENL + T +G Q Q + L Sbjct: 71 ETELTRLPMTQRARLGIGYLAQEASIFRHLTVVENLLLVLQQTGIRGREQRQRVEELLSE 130 Query: 121 PRLKERFTQRGGTMSGGEQQMLAIGRALM---SKPKLLLLDEPSLGLAPIIIQQIFDIIE 177 RL++ +G +SGGE++ I RAL PK LLLDEP G+ PI + ++ +II Sbjct: 131 FRLEKVAHTQGIRVSGGERRRTEIARALAVGSQGPKFLLLDEPFAGIDPIAVAEVQEIIA 190 Query: 178 QLRKDGVTVFLVEQNANQALKIADRAYVLENGRVVMQGTGEALLTDPKVREAYLG 232 +LR + + + + N + LKI DRAY+L +G ++ G+ E L ++P VR+ YLG Sbjct: 191 RLRAQQMGILITDHNVRETLKITDRAYILRDGEILAAGSAEELASNPLVRQYYLG 245 Lambda K H 0.320 0.137 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 134 Number of extensions: 9 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 233 Length of database: 250 Length adjustment: 23 Effective length of query: 210 Effective length of database: 227 Effective search space: 47670 Effective search space used: 47670 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory