Alignments for a candidate for ams in Synechococcus elongatus PCC 7942

GapMind for catabolism of small carbon sources

Alignments for a candidate for ams in Synechococcus elongatus PCC 7942

Align neutral invertase (InvA; sll0626) (EC 3.2.1.26) (characterized)
to candidate Synpcc7942_0397 Synpcc7942_0397 putative neutral invertase

Query= CAZy::CAD33848.1
         (489 letters)



>FitnessBrowser__SynE:Synpcc7942_0397
          Length = 463

 Score =  402 bits (1034), Expect = e-116
 Identities = 201/458 (43%), Positives = 284/458 (62%), Gaps = 25/458 (5%)

Query: 7   QQILDQARRLLYEKAMVKINGQYVGTVAAIPQSDH-HDLNYTEVFIRDNVPVMIFLLLQN 65
           Q  L  A +LL+++A+V+ + Q+ G +AA+P+       NY E+FIRDNVPVM++LLLQ 
Sbjct: 12  QTALQTAEQLLWDRALVRYHDQWAGAIAALPEDQELAAANYREIFIRDNVPVMLYLLLQG 71

Query: 66  ETEIVQNFLEICLTLQSKGFPTYGIFPTSFVETENHELKADYGQRAIGRVCSVDASLWWP 125
           +T++V++FL++ L+LQS+   TYGI PTSFV  E H + ADYGQRAIGRV S D SLWWP
Sbjct: 72  KTDVVRDFLQLSLSLQSQALQTYGILPTSFVCEETHCV-ADYGQRAIGRVVSADPSLWWP 130

Query: 126 ILAYYYVQRTGNEAWARQTHVQLGLQKFLNLILHPVFRDAPTLFVPDGAFMIDRPMDVWG 185
           +L   Y + + ++A+     VQ GLQ+ L  +L PVF   P L VPDGAFM+DRP+DV G
Sbjct: 131 VLLQAYRRASHDDAFVHSPTVQQGLQRLLAFLLRPVFNQNPLLEVPDGAFMVDRPLDVAG 190

Query: 186 APLEIQTLLYGALKSAAGLLLIDLKAKGYCSNKDHPFDSFTMEQSHQFNLSVDWLKKLRT 245
           APLEIQ LLYGAL++   LL                      + +   N +    ++LR 
Sbjct: 191 APLEIQVLLYGALRACGQLL----------------------QYTEAANAAHVQARRLRQ 228

Query: 246 YLLKHYWINCNIVQALRRRPTEQYGEEASNEHNVHTETIPNWLQDWLGDRGGYLIGNIRT 305
           YL  HYW+  + ++  ++ PTE++G+ + N +N+    IP+W++ WLG+ GGY +GNIR 
Sbjct: 229 YLCWHYWVTPDRLRRWQQWPTEEFGDRSHNPYNIQPIAIPDWVEPWLGESGGYFLGNIRA 288

Query: 306 GRPDFRFFSLGNCLGAIFDVTSLAQQRSFFRLVLNNQRELCAQMPLRICHPPLKDDDWRS 365
           GRPDFRFFSLGN L  +FDV  L QQ +  RL+L N+ ++  Q+PLR+C+P L    W+ 
Sbjct: 289 GRPDFRFFSLGNLLAIVFDVLPLNQQGAILRLILQNEAQILGQVPLRLCYPALTGSAWKI 348

Query: 366 KTGFDRKNLPWCYHNAGHWPCLFWFLVVAVLRHSCHSNYGTVEYAEMGNLIRNNYEVLLR 425
            TG D KN PW YHN G WP L W+L  AVL +        +    + N +++ +     
Sbjct: 349 LTGCDPKNQPWSYHNGGSWPSLLWYLSAAVLHYQQRGGDRNLCQVWL-NKLQHYHTQQCE 407

Query: 426 RLPKHKWAEYFDGPTGFWVGQQSRSYQTWTIVGLLLVH 463
           +LP  +W EY++G     +  ++  YQTWT  GLLL H
Sbjct: 408 QLPGDEWPEYYEGQDSVQIATRACRYQTWTFTGLLLNH 445


Lambda     K      H
   0.324    0.139    0.455 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 736
Number of extensions: 37
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 489
Length of database: 463
Length adjustment: 33
Effective length of query: 456
Effective length of database: 430
Effective search space:   196080
Effective search space used:   196080
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources