Align lactoylglutathione lyase (EC 4.4.1.5) (characterized)
to candidate Synpcc7942_0638 Synpcc7942_0638 Glyoxalase I
Query= BRENDA::P0A0T3 (138 letters) >FitnessBrowser__SynE:Synpcc7942_0638 Length = 137 Score = 197 bits (500), Expect = 6e-56 Identities = 90/125 (72%), Positives = 106/125 (84%) Query: 1 MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFVGYGDETDSTVLELTHN 60 MRLLHTMLRVG+LE+SL FY +LGM+LLRRKDYP G FTLAFVGYG+E D TVLELT+N Sbjct: 1 MRLLHTMLRVGDLERSLQFYCEILGMQLLRRKDYPGGEFTLAFVGYGEEADHTVLELTYN 60 Query: 61 WDTERYDLGNAYGHIAVEVDDAYEACERVKRQGGNVVREAGPMKHGTTVIAFVEDPDGYK 120 W E+Y+LG+AYGHIA+ VDD Y CE ++ +GG + RE GPMKHG+TVIAFVEDPDGYK Sbjct: 61 WGKEQYELGDAYGHIAIGVDDIYATCEAIRARGGKISREPGPMKHGSTVIAFVEDPDGYK 120 Query: 121 IEFIQ 125 +E IQ Sbjct: 121 VELIQ 125 Lambda K H 0.318 0.137 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 125 Number of extensions: 1 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 138 Length of database: 137 Length adjustment: 15 Effective length of query: 123 Effective length of database: 122 Effective search space: 15006 Effective search space used: 15006 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 42 (20.8 bits)
Align candidate Synpcc7942_0638 Synpcc7942_0638 (Glyoxalase I)
to HMM TIGR00068 (gloA: lactoylglutathione lyase (EC 4.4.1.5))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR00068.hmm # target sequence database: /tmp/gapView.21878.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00068 [M=150] Accession: TIGR00068 Description: glyox_I: lactoylglutathione lyase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.8e-69 218.1 0.0 2.1e-69 217.9 0.0 1.0 1 lcl|FitnessBrowser__SynE:Synpcc7942_0638 Synpcc7942_0638 Glyoxalase I Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__SynE:Synpcc7942_0638 Synpcc7942_0638 Glyoxalase I # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 217.9 0.0 2.1e-69 2.1e-69 17 145 .. 2 130 .. 1 134 [. 0.97 Alignments for each domain: == domain 1 score: 217.9 bits; conditional E-value: 2.1e-69 TIGR00068 17 lllhtmlrvgdldksldfytevlGmkllrkkdfpeekfslaflgyedessaavieLtynwgtekydlGn 85 +llhtmlrvgdl++sl+fy+e+lGm+llr+kd+p +f+laf+gy++e++++v+eLtynwg+e+y+lG lcl|FitnessBrowser__SynE:Synpcc7942_0638 2 RLLHTMLRVGDLERSLQFYCEILGMQLLRRKDYPGGEFTLAFVGYGEEADHTVLELTYNWGKEQYELGD 70 79******************************************************************* PP TIGR00068 86 gfGhiaiavddvykacervkakGgkvvrepgpvkggtkviafvkDPDGykiellekkktk 145 ++Ghiai+vdd+y++ce+++a+Ggk++repgp+k+g++viafv+DPDGyk+el+++++ lcl|FitnessBrowser__SynE:Synpcc7942_0638 71 AYGHIAIGVDDIYATCEAIRARGGKISREPGPMKHGSTVIAFVEDPDGYKVELIQTGTSG 130 *******************************************************98865 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (150 nodes) Target sequences: 1 (137 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00 # Mc/sec: 4.88 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory