Align NatE, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized)
to candidate Synpcc7942_1893 Synpcc7942_1893 ATPase
Query= TCDB::Q8YT15 (247 letters) >FitnessBrowser__SynE:Synpcc7942_1893 Length = 258 Score = 140 bits (353), Expect = 2e-38 Identities = 81/250 (32%), Positives = 138/250 (55%), Gaps = 20/250 (8%) Query: 11 LLEVENVHAGYIKDVDILQGVNFRVESGELVTVIGPNGAGKSTLAKTIFGLLTPHTGKIT 70 LLE E + + + +Q V +V G + +IGPNGAGKSTL + L GK+ Sbjct: 7 LLEAEGLSKRF-GGLQAVQDVRLQVAEGTITGLIGPNGAGKSTLFALLSNFLKADAGKVR 65 Query: 71 FKGKNIAGLKSNQIVRLGMCYVPQIANVFPSLSVEENLEMGA----------------FI 114 F+G+ I L+ Q+ +LG+ Q+A L+V EN+ +G I Sbjct: 66 FRGRAIEKLQPYQLAQLGLVRTFQVARSLSRLTVLENMLLGGQQQRGEKFWQVWLHPQAI 125 Query: 115 RNDSLQPLKDKIFAMFPR--LSDRRRQRAGTLSGGERQMLAMGKALMLEPSLLVLDEPSA 172 R Q L+D+ F++ L+ + + AG+LSGG+R++L M +ALM +P L++LDEP+A Sbjct: 126 RRQE-QELRDRAFSLLSDVGLAAKAQDYAGSLSGGQRKLLEMARALMAQPQLVLLDEPAA 184 Query: 173 ALSPILVTQVFEQVKQINQEGTAIILVEQNARKALEMADRGYVLESGRDAISGPGQELLT 232 ++P L+ ++ + ++ NQ+G + +++E N + + DR +VL G++ + GP ++ Sbjct: 185 GVNPALIEKICDHIQTWNQQGISFLIIEHNMDVIMSLCDRVWVLAEGKNLVDGPPAQIQQ 244 Query: 233 DPKVAELYLG 242 D +V YLG Sbjct: 245 DEQVLAAYLG 254 Lambda K H 0.317 0.136 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 153 Number of extensions: 7 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 247 Length of database: 258 Length adjustment: 24 Effective length of query: 223 Effective length of database: 234 Effective search space: 52182 Effective search space used: 52182 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory