Align 4-hydroxybenzoate 3-monooxygenase (EC 1.14.13.2) (characterized)
to candidate GFF1441 PS417_07330 4-hydroxybenzoate 3-monooxygenase
Query= reanno::pseudo6_N2E2:Pf6N2E2_2914 (396 letters) >FitnessBrowser__WCS417:GFF1441 Length = 397 Score = 660 bits (1703), Expect = 0.0 Identities = 321/397 (80%), Positives = 359/397 (90%), Gaps = 1/397 (0%) Query: 1 MKTLKTQVAIIGAGPSGLLLGQLLHNAGIDTVILERQTPEYVLSRIRAGVLEQGMVELLR 60 MKTLKTQVAIIGAGPSGLLLGQLLH AGI+T+ILERQ+ +YV RIRAGVLEQGMV+LLR Sbjct: 1 MKTLKTQVAIIGAGPSGLLLGQLLHEAGIETLILERQSADYVQGRIRAGVLEQGMVDLLR 60 Query: 61 QAGVGQRMDAEGLPHDGFELVLNDRRVHIDLKGLTGGKNVMVYGQTEVTRDLMAAREAAG 120 QAGV +RMD+EGL HDGFEL LND+ HIDLKGLTGG++VM+YGQTEVTRDLMAAR AAG Sbjct: 61 QAGVSRRMDSEGLVHDGFELALNDQLTHIDLKGLTGGQSVMIYGQTEVTRDLMAARTAAG 120 Query: 121 ARTLYLASNAQPHDMQTQTPFVTFEHEGETWRLDCDYIAGCDGFHGVARQSIPAEKLKVF 180 A TLY A N QPH +++ P++TFEH+GE +RL+CDYIAGCDG+HGVARQSIPAE LKVF Sbjct: 121 ATTLYEARNVQPHGLKSDRPWLTFEHQGEAFRLECDYIAGCDGYHGVARQSIPAESLKVF 180 Query: 181 ERVYPFGWLGVLADTPPVHEELVYARHTRGFALCSMRSKTRTRYYLQVPAEEQVADWPDE 240 ERVYPFGWLG+LADTPPVH ELVYA+H+RGFALCSMRS TR+RYYLQVP EE + +W D Sbjct: 181 ERVYPFGWLGILADTPPVHAELVYAKHSRGFALCSMRSPTRSRYYLQVPVEEPIDEWSDA 240 Query: 241 RFWGELKNRLPADLAAALVTGPSIEKSIAPLRSFVVEPMQYGRMFLVGDAAHIVPPTGAK 300 RFW ELK RLP+ LA LVTGPSIEKSIAPLRSFVVEPMQYGR+FL+GDAAHIVPPTGAK Sbjct: 241 RFWDELKTRLPSHLAEQLVTGPSIEKSIAPLRSFVVEPMQYGRLFLLGDAAHIVPPTGAK 300 Query: 301 GLNLAASDVSTLFNILLKVYRDGRVELLEKYSAICLRRVWKAERFSWWMTSMLHRFDD-D 359 GLNLAASDVSTL+ ILLKVY +GRV+LLE+YSAICLRRVWKAERFSWWMTSMLH+F + D Sbjct: 301 GLNLAASDVSTLYRILLKVYGEGRVDLLERYSAICLRRVWKAERFSWWMTSMLHQFPEAD 360 Query: 360 AFNQRISEAELEYFVDSEAGRKTIAENYVGLPYEAIE 396 F QRI+E+ELEYF+ SEAGRKTIAENYVGLPYEAIE Sbjct: 361 GFTQRIAESELEYFIHSEAGRKTIAENYVGLPYEAIE 397 Lambda K H 0.321 0.137 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 608 Number of extensions: 12 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 396 Length of database: 397 Length adjustment: 31 Effective length of query: 365 Effective length of database: 366 Effective search space: 133590 Effective search space used: 133590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 50 (23.9 bits)
Align candidate GFF1441 PS417_07330 (4-hydroxybenzoate 3-monooxygenase)
to HMM TIGR02360 (pobA: 4-hydroxybenzoate 3-monooxygenase (EC 1.14.13.2))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR02360.hmm # target sequence database: /tmp/gapView.26441.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR02360 [M=390] Accession: TIGR02360 Description: pbenz_hydroxyl: 4-hydroxybenzoate 3-monooxygenase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 7e-208 676.1 0.0 8.1e-208 675.9 0.0 1.0 1 lcl|FitnessBrowser__WCS417:GFF1441 PS417_07330 4-hydroxybenzoate 3- Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__WCS417:GFF1441 PS417_07330 4-hydroxybenzoate 3-monooxygenase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 675.9 0.0 8.1e-208 8.1e-208 1 390 [] 4 393 .. 4 393 .. 1.00 Alignments for each domain: == domain 1 score: 675.9 bits; conditional E-value: 8.1e-208 TIGR02360 1 mktqvaiigaGpsGlllgqllhkaGidavilerksrdyvlgriraGvleqgtvdlleeagvderldreglvheGv 75 +ktqvaiigaGpsGlllgqllh+aGi+++iler+s+dyv+griraGvleqg+vdll++agv++r+d eglvh+G+ lcl|FitnessBrowser__WCS417:GFF1441 4 LKTQVAIIGAGPSGLLLGQLLHEAGIETLILERQSADYVQGRIRAGVLEQGMVDLLRQAGVSRRMDSEGLVHDGF 78 69************************************************************************* PP TIGR02360 76 eiafegekvrvdlkkltggksvlvyGqtevtrdlyeareaaglktvyeadevrlhdlesdrpkvtfekdgeekrl 150 e+a +++ +++dlk ltgg+sv++yGqtevtrdl+ ar aag++t+yea +v++h l+sdrp +tfe++ge rl lcl|FitnessBrowser__WCS417:GFF1441 79 ELALNDQLTHIDLKGLTGGQSVMIYGQTEVTRDLMAARTAAGATTLYEARNVQPHGLKSDRPWLTFEHQGEAFRL 153 *************************************************************************** PP TIGR02360 151 dcdfiaGcdGfhGvsrksipaeklkefekvypfGwlGilsetppvsdeliysnserGfalcslrsetrsryyvqv 225 +cd+iaGcdG+hGv+r+sipae lk+fe+vypfGwlGil++tppv+ el+y++++rGfalcs+rs trsryy+qv lcl|FitnessBrowser__WCS417:GFF1441 154 ECDYIAGCDGYHGVARQSIPAESLKVFERVYPFGWLGILADTPPVHAELVYAKHSRGFALCSMRSPTRSRYYLQV 228 *************************************************************************** PP TIGR02360 226 sltdkvedwsddrfweelkrrldeeaaeklvtgpsieksiaplrsfvaepmryGrlflaGdaahivpptGakGln 300 ++++ +++wsd+rfw+elk+rl+++ ae+lvtgpsieksiaplrsfv+epm+yGrlfl GdaahivpptGakGln lcl|FitnessBrowser__WCS417:GFF1441 229 PVEEPIDEWSDARFWDELKTRLPSHLAEQLVTGPSIEKSIAPLRSFVVEPMQYGRLFLLGDAAHIVPPTGAKGLN 303 *************************************************************************** PP TIGR02360 301 laasdvaylyealleaykekdsaglerysakalarvwkaerfswwltsllhrfpdedefdkkiqqaeleyllese 375 laasdv++ly+ ll+ y e++ + lerysa +l+rvwkaerfsww+ts+lh+fp+ d f ++i ++eley+ +se lcl|FitnessBrowser__WCS417:GFF1441 304 LAASDVSTLYRILLKVYGEGRVDLLERYSAICLRRVWKAERFSWWMTSMLHQFPEADGFTQRIAESELEYFIHSE 378 *************************************************************************** PP TIGR02360 376 aaqktlaenyvGlpy 390 a++kt+aenyvGlpy lcl|FitnessBrowser__WCS417:GFF1441 379 AGRKTIAENYVGLPY 393 **************9 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (390 nodes) Target sequences: 1 (397 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.02 # Mc/sec: 7.74 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory