Alignments for a candidate for nagF in Pseudomonas simiae WCS417

GapMind for catabolism of small carbon sources

Alignments for a candidate for nagF in Pseudomonas simiae WCS417

Align N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) (characterized)
to candidate GFF5270 PS417_26985 phosphoenolpyruvate-protein phosphotransferase

Query= reanno::BFirm:BPHYT_RS02740
         (854 letters)



>FitnessBrowser__WCS417:GFF5270
          Length = 759

 Score =  278 bits (711), Expect = 9e-79
 Identities = 183/576 (31%), Positives = 300/576 (52%), Gaps = 22/576 (3%)

Query: 285 GETLAPNTLAGVCAAPGVAVGK-LVRWDDADIDP-PEKANGTSAAESRLLDKAIATVDAD 342
           G+ +      GV  +PG AVG  +V    AD+D  P+K      AE +L   A+  V AD
Sbjct: 170 GKGIQEAKFVGVPGSPGAAVGTAVVMLPPADLDVVPDKTVSDIDAEIKLFKTALEGVRAD 229

Query: 343 LDTTVRDASQRGAVGEAGIFSVHRVLLEDPTLLDAARDLISLGKSAGFAWREAIRAQIAI 402
           +       + +    E  +F V++++L+D +L +  + +I  G+ A  A R+ +   +  
Sbjct: 230 MRNLSNKLATQLRPEERALFDVYQMMLDDASLGNEVKTVIKTGQWAQGALRQVVTDHVNR 289

Query: 403 LTNIEDALLAERAADLRDIEKRVLRALGYTSATARTLPEEAVLAAEEFTPSDLSTLDRSR 462
              ++D  L ERA+D+RD+ +R+L  L     T    P+  +L +EE T + L  +   +
Sbjct: 290 FEMMDDDYLRERASDVRDLGRRLLAKLQEDRTTNLVYPDNTILVSEELTATMLGEVPEGK 349

Query: 463 VTALVMARGGATSHAAILARQAGIPALVAVGDALHAIPEGTQVVVNATTGRLEFAPTELD 522
           +  LV   G   SH AILAR  GIP ++ + D  +A  +G  ++V+   G +   P+E+ 
Sbjct: 350 LVGLVSVLGSGNSHVAILARAMGIPTVMGLVDLPYAKVDGIGMIVDGHRGEVFTNPSEV- 408

Query: 523 VERARLERTRLADVREANRRTS-------QQAAVTSDGRAIEVAANIATLDDAKTAVENG 575
                  R + A+V E  ++ +       +   VT+DG  + +  N   L D   A + G
Sbjct: 409 ------LRKQYAEVVEEEKQLALGLDSLRELPCVTTDGHRVPLLVNTGLLADVARAQQRG 462

Query: 576 ADSVGLLRTELLFIHRAAAPTTDEHRQSYQAIVDALSGRTAIIRTLDVGADKEVDYLTLP 635
           A+ VGL RTE+ F+     P+  E    Y+  + A       +R+LD+G DK + Y  + 
Sbjct: 463 AEGVGLYRTEVPFMINQRFPSEKEQLAIYREQLQAFHPLPVTMRSLDIGGDKSLSYFPIK 522

Query: 636 PEPNPALGLRGIRLAQVRPDLLDDQLRGLL-AVQPLGAVRILLPMVTDVGEL---IRIRK 691
            E NP LG RGIR+    P++   Q R +L A + L  +RILLPM++   EL   + +  
Sbjct: 523 -EDNPFLGWRGIRVTLDHPEIFLVQTRAMLKASEGLNNLRILLPMISGTHELEEALHLIH 581

Query: 692 RIDEFARELGRTEPIE-VGVMIEVPSAALLADQLAQHADFLSIGTNDLTQYTLAMDRCQA 750
           R     R+ G   P+  +GVMIE+P+A   A +LA+  DFLS+G+NDLTQY LA+DR   
Sbjct: 582 RAWGEVRDEGADVPMPPIGVMIEIPAAVYQAKELARQVDFLSVGSNDLTQYLLAVDRNNP 641

Query: 751 DLAAQADGLHPAVLRLIAATVQGADKHGKWVGVCGALAGDPLAMPLLVGLGVTELSVDPV 810
            +A   D LHPAVL+ +   V+ A   GK V +CG +AGDP A  LL+ +G   LS++  
Sbjct: 642 RVADLYDYLHPAVLQALQHVVRDAHAEGKPVSICGEMAGDPAAAVLLMAMGFDSLSMNAT 701

Query: 811 SVPGIKARVRNLDYQLCRQRAQDALALESAQAVRAA 846
           ++P +K  +R ++  + +    + + +++ Q + ++
Sbjct: 702 NLPKVKWMLRQVELSMAKDLLAELMTIDNPQVIHSS 737


Lambda     K      H
   0.317    0.133    0.371 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1274
Number of extensions: 59
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 854
Length of database: 759
Length adjustment: 41
Effective length of query: 813
Effective length of database: 718
Effective search space:   583734
Effective search space used:   583734
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources