Align Agmatine deiminase (EC 3.5.3.12) (characterized)
to candidate GFF263 PS417_01340 agmatine deiminase
Query= reanno::pseudo5_N2C3_1:AO356_10030 (368 letters) >FitnessBrowser__WCS417:GFF263 Length = 368 Score = 711 bits (1834), Expect = 0.0 Identities = 333/366 (90%), Positives = 352/366 (96%) Query: 1 MTTLHSTPRADGFHMPAEWATQTQVWMIWPERPDNWRLGGKPAQAAHVAVAKAIARFEPV 60 MTTLHSTPRADGF MPAEWA QTQVWMIWPERPDNWRLGGKPAQAAHVAVAKAIARFEPV Sbjct: 1 MTTLHSTPRADGFRMPAEWAPQTQVWMIWPERPDNWRLGGKPAQAAHVAVAKAIARFEPV 60 Query: 61 TVAVSAAQYENARARLDVPNIRLVEMSSDDAWVRDTGPTFVINDSGEVRGVDWDFNAWGG 120 TVAVSA QYENARARLDVPNIR+VEMSSDDAWVRDTGPTFVINDSGEVRGV+WDFNAWGG Sbjct: 61 TVAVSAGQYENARARLDVPNIRVVEMSSDDAWVRDTGPTFVINDSGEVRGVNWDFNAWGG 120 Query: 121 FDGGLYAPWNRDSQVASKVLEIERSPRYRTEGFVLEGGSIHVDGEGTVITTEECLLNRNR 180 FDGGLY+PWNRDSQV K+LEIER+PRYRTEGFVLEGGSIHVDGEGT+ITTEECLLNRNR Sbjct: 121 FDGGLYSPWNRDSQVGGKILEIERTPRYRTEGFVLEGGSIHVDGEGTLITTEECLLNRNR 180 Query: 181 NPHMSREQIEAVLSAQLAVDKVIWLPDGLFNDETDGHVDNFCCYVRPGEVLLAWTDDPQN 240 NPH+ R +IEAVLSA LAVDK+IWLPDGLFNDETDGHVDNFCCYVRPGEVLLAWTDDPQ+ Sbjct: 181 NPHLDRAEIEAVLSANLAVDKIIWLPDGLFNDETDGHVDNFCCYVRPGEVLLAWTDDPQD 240 Query: 241 PNYTRCHAAMDVLQNSTDAKGRPFTVHKMPIPGPMYATEEECAGVDAVEGSQERNPTVRL 300 PNY RCHAAMDVLQ+STDA+GR FTVHKMPIPGP+YATEEEC GVD V+G+QERNPTVRL Sbjct: 241 PNYARCHAAMDVLQSSTDAQGRSFTVHKMPIPGPLYATEEECEGVDPVDGTQERNPTVRL 300 Query: 301 AGSYVNFLIVNGGIIAPSFDDPLDAPARDILQNLFPQHEVVMVPGRELLLGGGNIHCLTQ 360 AGSYVNFLIVNGGIIAPSFDDPLD+ A+ ILQ+LFPQHEVVMVPGRELLLGGGNIHCLTQ Sbjct: 301 AGSYVNFLIVNGGIIAPSFDDPLDSQAKAILQDLFPQHEVVMVPGRELLLGGGNIHCLTQ 360 Query: 361 QQPAPY 366 QQPAP+ Sbjct: 361 QQPAPH 366 Lambda K H 0.318 0.136 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 683 Number of extensions: 16 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 368 Length of database: 368 Length adjustment: 30 Effective length of query: 338 Effective length of database: 338 Effective search space: 114244 Effective search space used: 114244 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 49 (23.5 bits)
Align candidate GFF263 PS417_01340 (agmatine deiminase)
to HMM TIGR03380 (aguA: agmatine deiminase (EC 3.5.3.12))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR03380.hmm # target sequence database: /tmp/gapView.15334.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR03380 [M=357] Accession: TIGR03380 Description: agmatine_aguA: agmatine deiminase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.5e-178 579.1 0.0 1.7e-178 578.9 0.0 1.0 1 lcl|FitnessBrowser__WCS417:GFF263 PS417_01340 agmatine deiminase Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__WCS417:GFF263 PS417_01340 agmatine deiminase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 578.9 0.0 1.7e-178 1.7e-178 1 357 [] 6 364 .. 6 364 .. 0.99 Alignments for each domain: == domain 1 score: 578.9 bits; conditional E-value: 1.7e-178 TIGR03380 1 ttpkedgfrlPaefekqkevwliWPerpDnWregakpaqkafaevakaiakvepvtvlvskeqyenarkalpe.ei 75 +tp++dgfr+Pae+++q++vw+iWPerpDnWr g+kpaq+a+++vakaia++epvtv+vs+ qyenar++l+ +i lcl|FitnessBrowser__WCS417:GFF263 6 STPRADGFRMPAEWAPQTQVWMIWPERPDNWRLGGKPAQAAHVAVAKAIARFEPVTVAVSAGQYENARARLDVpNI 81 58*********************************************************************966** PP TIGR03380 76 rvvemssnDawirDvGPtfvvndkgelrgvdwefnawgglkdGlyfpWdkDdkvarkvleleridryra.dlvleg 150 rvvemss+Daw+rD+GPtfv+nd+ge+rgv+w+fnawgg+++Gly+pW++D++v k+le+er++ryr+ +vleg lcl|FitnessBrowser__WCS417:GFF263 82 RVVEMSSDDAWVRDTGPTFVINDSGEVRGVNWDFNAWGGFDGGLYSPWNRDSQVGGKILEIERTPRYRTeGFVLEG 157 *********************************************************************89***** PP TIGR03380 151 gsihvDGeGtlltteeclLsegrnpelskeeieeklkeylgvekviWlkeGlyldetnGhvDnllnfvrPGevvls 226 gsihvDGeGtl+tteeclL+++rnp+l ++eie++l + l+v+k+iWl++Gl++det+GhvDn++++vrPGev+l+ lcl|FitnessBrowser__WCS417:GFF263 158 GSIHVDGEGTLITTEECLLNRNRNPHLDRAEIEAVLSANLAVDKIIWLPDGLFNDETDGHVDNFCCYVRPGEVLLA 233 **************************************************************************** PP TIGR03380 227 wtddesdPqyeiskealevLenetDakGrklkvhklklpeplllteeeakgvdavegtlpreagerlaasyvnyli 302 wtdd +dP+y+++++a++vL+++tDa+Gr ++vhk+++p+pl++teee++gvd v+gt++r++ rla+syvn+li lcl|FitnessBrowser__WCS417:GFF263 234 WTDDPQDPNYARCHAAMDVLQSSTDAQGRSFTVHKMPIPGPLYATEEECEGVDPVDGTQERNPTVRLAGSYVNFLI 309 **************************************************************************** PP TIGR03380 303 ankaiilPlfddekDkeakkllqelfPdrkvvgvkareillgGGnihcitqqipa 357 +n++ii+P+fdd+ D++ak++lq+lfP+++vv+v+ re+llgGGnihc+tqq+pa lcl|FitnessBrowser__WCS417:GFF263 310 VNGGIIAPSFDDPLDSQAKAILQDLFPQHEVVMVPGRELLLGGGNIHCLTQQQPA 364 *****************************************************96 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (357 nodes) Target sequences: 1 (368 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 7.98 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory