Align Probable 2-ketoarginine decarboxylase AruI; 2-oxo-5-guanidinopentanoate decarboxylase; 5-guanidino-2-oxopentanoate decarboxylase; EC 4.1.1.75 (characterized)
to candidate GFF2097 PS417_10695 decarboxylase
Query= SwissProt::Q9HUI8 (559 letters) >FitnessBrowser__WCS417:GFF2097 Length = 535 Score = 858 bits (2216), Expect = 0.0 Identities = 423/531 (79%), Positives = 471/531 (88%) Query: 28 KTLTAGQALVRLLANYGVDTVFGIPGVHTLELYRGLPGSGIRHVLTRHEQGAGFMADGYA 87 K +T GQALVRLLANYGVDTVFGIPGVHTLELYRGLPGSGIRHVLTRHEQGAGFMADGYA Sbjct: 4 KAMTGGQALVRLLANYGVDTVFGIPGVHTLELYRGLPGSGIRHVLTRHEQGAGFMADGYA 63 Query: 88 RVSGKPGVCFVITGPGVTNVATAIGQAYADSVPLLVISSVNHSASLGKGWGCLHETQDQR 147 RVSGKPGVCFVITGPGVTN ATAIGQAYADS+P+LVISSVN + SLGKGWG LHETQDQR Sbjct: 64 RVSGKPGVCFVITGPGVTNAATAIGQAYADSIPMLVISSVNSTVSLGKGWGILHETQDQR 123 Query: 148 AMTAPITAFSALALSPEQLPELIARAYAVFDSERPRPVHISIPLDVLAAPVAHDWSAAVA 207 A+TAPITAFSA+ALS E LPEL+ARAYA+FDSERPRPVHIS+PLDVLAAP+ DWS V Sbjct: 124 AITAPITAFSAVALSAEDLPELVARAYAIFDSERPRPVHISVPLDVLAAPIQRDWSNEVV 183 Query: 208 RRPGRGVPCSEALRAAAERLAAARRPMLIAGGGALAAGEALAALSERLAAPLFTSVAGKG 267 RRP RG+P ++AL A +LAAA+RPM+IAGGGALAAGEAL LS +LAAP FTSVAGKG Sbjct: 184 RRPDRGLPPAKALDQGAAKLAAAKRPMIIAGGGALAAGEALQRLSTQLAAPFFTSVAGKG 243 Query: 268 LLPPDAPLNAGASLCVAPGWEMIAEADLVLAVGTEMADTDFWRERLPLSGELIRVDIDPR 327 LLP + PLNAG++LCV PGW++I+EAD+VLAVGTEMADTD+W ERLPL GEL+RVDID R Sbjct: 244 LLPVNDPLNAGSTLCVEPGWQLISEADVVLAVGTEMADTDYWCERLPLGGELLRVDIDAR 303 Query: 328 KFNDFYPSAVALRGDARQTLEALLVRLPQEARDSAPAAARVARLRAEIRAAHAPLQALHQ 387 KFNDFYP AVAL+GDA+QT+ ALL RLP AR++ A + VA L I + H PLQ++HQ Sbjct: 304 KFNDFYPCAVALKGDAQQTVLALLERLPTTARNAEAAISTVAALLHAIESGHGPLQSIHQ 363 Query: 388 AILDRIAAALPADAFVSTDMTQLAYTGNYAFASRAPRSWLHPTGYGTLGYGLPAGIGAKL 447 AILDRI A LPA+AF+S+DMTQLAYTGNYA+ SRAPRSWLHPTGYGTLGYGLPAGIGAK Sbjct: 364 AILDRIEAELPANAFISSDMTQLAYTGNYAYRSRAPRSWLHPTGYGTLGYGLPAGIGAKF 423 Query: 448 GAPQRPGLVLVGDGGFLYTAQELATASEELDSPLVVLLWNNDALGQIRDDMLGLDIEPVG 507 GAPQRPGLVLVGDGGFLYTAQELATA EELDSPLVVLLWNNDALGQIRDDML LDI P+G Sbjct: 424 GAPQRPGLVLVGDGGFLYTAQELATAVEELDSPLVVLLWNNDALGQIRDDMLSLDIAPIG 483 Query: 508 VLPRNPDFALLGRAYGCAVRQPQDLDELERDLRAGFGQSGVTLIELRHACA 558 VLPRNPDFAL +A+GC V QP++LDEL+ DLR F ++GVTLIEL+HACA Sbjct: 484 VLPRNPDFALFAQAFGCTVNQPRNLDELQTDLRNAFKRNGVTLIELKHACA 534 Lambda K H 0.321 0.136 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1004 Number of extensions: 36 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 559 Length of database: 535 Length adjustment: 36 Effective length of query: 523 Effective length of database: 499 Effective search space: 260977 Effective search space used: 260977 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory