Align ABC transporter for L-Arginine and L-Citrulline, ATPase component (characterized)
to candidate GFF4241 PS417_21725 amino acid transporter
Query= reanno::pseudo1_N1B4:Pf1N1B4_3435 (254 letters) >FitnessBrowser__WCS417:GFF4241 Length = 254 Score = 477 bits (1228), Expect = e-140 Identities = 241/254 (94%), Positives = 248/254 (97%) Query: 1 MYKLEVQDLHKRYGSHEVLKGVSLKAAAGDVISIIGSSGSGKSTFLRCINLLEQPHAGKI 60 MYKLEVQDLHKRYGSHEVLKGVSL AAAGDVISIIGSSGSGKSTFLRCINLLEQPHAGKI Sbjct: 1 MYKLEVQDLHKRYGSHEVLKGVSLAAAAGDVISIIGSSGSGKSTFLRCINLLEQPHAGKI 60 Query: 61 LLNNEELKLVANKDGALKAADPKQLQRMRSRLSMVFQHFNLWSHMTAMENIMEAPVHVLG 120 LLNNEELKLVANKDGA+KAADPKQLQRMRSRLSMVFQHFNLWSHMTA+EN+MEAPVHVLG Sbjct: 61 LLNNEELKLVANKDGAMKAADPKQLQRMRSRLSMVFQHFNLWSHMTAIENVMEAPVHVLG 120 Query: 121 MSKTEAREKAEHYLNKVGVAHRKDAYPGHMSGGEQQRVAIARALAMEPEVMLFDEPTSAL 180 +SK EAREKAEHYL KVGV HRKDA+PGHMSGGEQQRVAIARALAMEPEVMLFDEPTSAL Sbjct: 121 VSKKEAREKAEHYLAKVGVGHRKDAFPGHMSGGEQQRVAIARALAMEPEVMLFDEPTSAL 180 Query: 181 DPELVGDVLKVMQALAQEGRTMVVVTHEMGFAREVSNQLVFLHKGVVEESGNPREVLVNP 240 DPELVG+VLKVMQ LAQEGRTMVVVTHEMGFAREVSNQLVFLHKG+VEE GNPREVLVNP Sbjct: 181 DPELVGEVLKVMQDLAQEGRTMVVVTHEMGFAREVSNQLVFLHKGIVEERGNPREVLVNP 240 Query: 241 QSERLQQFLSGSLK 254 QSERLQQFLSGSLK Sbjct: 241 QSERLQQFLSGSLK 254 Lambda K H 0.317 0.131 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 334 Number of extensions: 7 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 254 Length of database: 254 Length adjustment: 24 Effective length of query: 230 Effective length of database: 230 Effective search space: 52900 Effective search space used: 52900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory