Alignments for a candidate for H281DRAFT_01113 in Pseudomonas simiae WCS417

GapMind for catabolism of small carbon sources

Alignments for a candidate for H281DRAFT_01113 in Pseudomonas simiae WCS417

Align deoxynucleoside transporter, ATPase component (characterized)
to candidate GFF2673 PS417_13635 D-ribose transporter ATP-binding protein

Query= reanno::Burk376:H281DRAFT_01113
         (515 letters)



>FitnessBrowser__WCS417:GFF2673
          Length = 510

 Score =  344 bits (882), Expect = 5e-99
 Identities = 200/488 (40%), Positives = 289/488 (59%), Gaps = 14/488 (2%)

Query: 13  QPFLEVVGVHKRFTGVHALRGVSLSFQRGQIYHLLGENGCGKSTLIKIISGAQPPDEG-Q 71
           +P LE+ G+ K F G+  L+ V L    G+I+ L+GENG GKSTL+KI+SGA   D G +
Sbjct: 4   EPLLEMQGISKTFNGLRVLKTVGLKVYPGEIHALMGENGAGKSTLMKILSGAYQADPGGE 63

Query: 72  LVIEGVPHARLSALEALAAGIETVYQDLSLLPNMSVAENVALTSELATHEGRLARTFDRR 131
           + I G          A A GI  +YQ+LSL PN+SVAEN+ L  EL     R   T DR+
Sbjct: 64  IRIAGQLIPTFDPATAKALGIAVIYQELSLCPNLSVAENIYLGREL-----RRGWTIDRK 118

Query: 132 VLAATAARALEAVGLPGNSEFQ-STLIEQLPLATRQLVAIARAIASEAKFVIMDEPTTSL 190
            + A     L+ +G    +EF  +T +  L +A RQLV IARA+ + AK ++MDEPTT L
Sbjct: 119 GMEAGCIEVLQRLG----AEFTPATRVSSLSIAERQLVEIARALHAHAKILVMDEPTTPL 174

Query: 191 TQKEVDNLIAVLANLRAQGVTVLFVSHKLDECYAIGGEVIVLRDGQKMAQGPIAEFTKAQ 250
           + +E D L A++  LR+QG+ ++++SH++ E YA+   V VLRDG  + +      +   
Sbjct: 175 SSRETDRLFALIKQLRSQGLAIIYISHRMAEIYALSDRVSVLRDGHYIGELTRDALSAEA 234

Query: 251 ISELMTGRHLSNERYRESA--HAQDIVLDVRGFTRAGQFSDVSFKLHGGEILGVTGLLDS 308
           + ++M GR LS    +E A  +  ++V+ VR      +    SF LH GE+LG+ GL+ +
Sbjct: 235 LVKMMVGRDLSGFYKKEHAAYNPGNVVMRVRDMADGKRVRHCSFDLHAGEVLGIAGLVGA 294

Query: 309 GRNELARALAGVAPAQSGDVLLDGQQIA-LRTPSDAKRHRIGYVPEDRLNEGLFLDKPIR 367
           GR ELAR +    P  SG + + G+ +  LRTP+DA R  + Y+ EDR  +GLFLD  + 
Sbjct: 295 GRTELARLIFAADPRTSGTLEVVGKAVTPLRTPADAIRAGVVYLTEDRKAQGLFLDMSVA 354

Query: 368 DNVITAMISSLRDRFGQIDRTRAQALAEQTVKELQIATPGVDKPVQSLSGGNQQRVLIGR 427
           DN+            G +DR  A   +   +K L I          +LSGGNQQ+VL+ R
Sbjct: 355 DNINVCACVPDAHAGGVLDRDHALQRSNDAIKSLSIRVASGKVNAGALSGGNQQKVLLAR 414

Query: 428 WLAIDPRVLILHGPTVGVDVGSKDIIYRIMQRLSQRGIGIILISDDLPELLQNCDRILMM 487
            L + P VLIL  PT GVD+GSK  IYRI+ +L+Q GIGI++IS +LPE++  CDR+L+M
Sbjct: 415 LLEVKPHVLILDEPTRGVDIGSKSEIYRIINQLAQAGIGIVVISSELPEIIGTCDRVLIM 474

Query: 488 KKGHVSAE 495
           ++G + AE
Sbjct: 475 REGQLVAE 482



 Score = 67.4 bits (163), Expect = 1e-15
 Identities = 59/222 (26%), Positives = 101/222 (45%), Gaps = 24/222 (10%)

Query: 290 VSFKLHGGEILGVTGLLDSGRNELARALAGVAPAQ-SGDVLLDGQQIALRTPSDAKRHRI 348
           V  K++ GEI  + G   +G++ L + L+G   A   G++ + GQ I    P+ AK   I
Sbjct: 25  VGLKVYPGEIHALMGENGAGKSTLMKILSGAYQADPGGEIRIAGQLIPTFDPATAKALGI 84

Query: 349 GYV-------PEDRLNEGLFLDKPIRDNVITAMISSLRDRFGQIDRTRAQALAEQTVKEL 401
             +       P   + E ++L + +R             R   IDR   +A   + ++ L
Sbjct: 85  AVIYQELSLCPNLSVAENIYLGRELR-------------RGWTIDRKGMEAGCIEVLQRL 131

Query: 402 QIA-TPGVDKPVQSLSGGNQQRVLIGRWLAIDPRVLILHGPTVGVDVGSKDIIYRIMQRL 460
               TP     V SLS   +Q V I R L    ++L++  PT  +     D ++ ++++L
Sbjct: 132 GAEFTPATR--VSSLSIAERQLVEIARALHAHAKILVMDEPTTPLSSRETDRLFALIKQL 189

Query: 461 SQRGIGIILISDDLPELLQNCDRILMMKKGHVSAEYRADELS 502
             +G+ II IS  + E+    DR+ +++ GH   E   D LS
Sbjct: 190 RSQGLAIIYISHRMAEIYALSDRVSVLRDGHYIGELTRDALS 231


Lambda     K      H
   0.319    0.135    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 646
Number of extensions: 32
Number of successful extensions: 10
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 515
Length of database: 510
Length adjustment: 35
Effective length of query: 480
Effective length of database: 475
Effective search space:   228000
Effective search space used:   228000
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources