Align Phosphate acetyltransferase; Phosphotransacetylase; EC 2.3.1.8 (characterized)
to candidate GFF734 PS417_03730 phosphate acetyltransferase
Query= SwissProt::Q9I5A5 (704 letters) >FitnessBrowser__WCS417:GFF734 Length = 699 Score = 1082 bits (2797), Expect = 0.0 Identities = 555/699 (79%), Positives = 615/699 (87%), Gaps = 8/699 (1%) Query: 1 MHTFFIAPTGFGVGLTSISLGLLRALERAGLKVGFFKPIAQLHPGDLGPERSSELVARTH 60 M TFFIAPT FGVGLTSISLGL+R LERAGLKVGFFKPIAQ HPGD GPERS+ELVARTH Sbjct: 1 MQTFFIAPTDFGVGLTSISLGLVRTLERAGLKVGFFKPIAQPHPGDTGPERSTELVARTH 60 Query: 61 GLDTPKPLPLAQVERMLGDGQLDELLEEIISLYQRAAADKDVVIVEGMVPTRHASYAARV 120 GL P+PL LA VERMLGDGQLDELLEEII+LYQ+AA KDV+IVEGMVPTR ASYAARV Sbjct: 61 GLKPPQPLGLAHVERMLGDGQLDELLEEIITLYQQAAVGKDVLIVEGMVPTRSASYAARV 120 Query: 121 NFHLAKSLDAEVILVSAPENETLTELTDRIEIQAQLFGGPRDPKVLGVILNKVRGEADAA 180 N HLAKSLDAEVILVSAPENE LTEL+ R+E+QAQLFGGP+DPKVLGVILNKVR Sbjct: 121 NLHLAKSLDAEVILVSAPENEVLTELSGRVELQAQLFGGPKDPKVLGVILNKVR------ 174 Query: 181 NAEDGVADFARRLTEHSPLLRD-DFRLIGCIPWQDELNAARTRDIADLLSARVINAGDYE 239 ++ + F+ RL EHSPLLR DFRL+GCIP+Q ELNA RTRD+ADL+ A+++NAGDYE Sbjct: 175 -TDESMEAFSARLKEHSPLLRSGDFRLLGCIPYQPELNAPRTRDVADLMGAQILNAGDYE 233 Query: 240 QRRVQKIVLCARAVPNTVQLLKPGVLVVTPGDRDDIILAASLAAMNGVPLAGLLLCSDFP 299 RR+ KI++CAR + NTV+LLKPGVLVVTPGDRDDIILA SLAA+NGVPLAGLLL SD Sbjct: 234 TRRMTKIIICARTMRNTVELLKPGVLVVTPGDRDDIILAVSLAAINGVPLAGLLLTSDTL 293 Query: 300 PDPRIMELCRGALQGGLPVLSVATGSYDTATNLNRMNKEIPVDDRERAERVTEFVAGHID 359 PDPRIMELCRGA Q GLPVLSV+TGSYDTA LN +NKEIP+DDRERAE +T+FVA H+D Sbjct: 294 PDPRIMELCRGAFQAGLPVLSVSTGSYDTANQLNSLNKEIPIDDRERAEIITDFVASHLD 353 Query: 360 FEWLKQRCGTPRELRLSPPAFRYQVVQRAQKAGKRIVLPEGSEPRTVQAAAICQARGIAR 419 WL QRCGTPRE+RLSP FRYQ++QRAQ A KRIVLPEGSEP TVQAAAICQARGIAR Sbjct: 354 ARWLHQRCGTPREMRLSPAVFRYQLIQRAQAANKRIVLPEGSEPLTVQAAAICQARGIAR 413 Query: 420 CVLLAKPEEVQAVAQAQGIVLPEGLEIIDPDLVRQRYVEPMVELRKGKGLNAPMAEQQLE 479 CVLLAKP +V+AVA+AQGI LPEGLEI+DPDL+RQRYVEPMV LRK K LNAPMAEQQLE Sbjct: 414 CVLLAKPADVEAVARAQGIELPEGLEILDPDLIRQRYVEPMVALRKSKSLNAPMAEQQLE 473 Query: 480 DSVVLATMMLALDEVDGLVSGAIHTTASTIRPALQLIKTAPGYNLVSSVFFMLLPDQVLV 539 D+VV+ATMMLALDEVDGLVSG IH+TA+TIRPALQLIKTAPG LVSSVFFML P+QVLV Sbjct: 474 DTVVIATMMLALDEVDGLVSGVIHSTANTIRPALQLIKTAPGCTLVSSVFFMLFPEQVLV 533 Query: 540 YGDCAVNPDPSASDLAEIAVQSAASAQAFGIPARVAMISYSTGDSGSGVDVDKVREATRL 599 YGDC +NP PSA++LAEIA+QSA SA AFGI RVAMISYS+GDS SG +V+KVREAT L Sbjct: 534 YGDCVMNPHPSAAELAEIALQSADSAAAFGITPRVAMISYSSGDSASGEEVEKVREATLL 593 Query: 600 AREQRPDLLIDGPLQYDAAAIASVGRQKAPNSPVAGQATVFIFPDLNTGNTTYKAVQRSA 659 A EQ+ LLIDGPLQYDAAA +V RQ APNS VAG+ATVF+FPDLNTGNTT+KAVQRSA Sbjct: 594 AHEQQSSLLIDGPLQYDAAANENVARQLAPNSQVAGKATVFVFPDLNTGNTTHKAVQRSA 653 Query: 660 DCVSVGPMLQGLRKPVNDLSRGALVEDIVYTIALTAIQA 698 DCVS+GPMLQGLRKPVNDL RGA V+DIVYTIALTAIQA Sbjct: 654 DCVSLGPMLQGLRKPVNDLPRGAQVDDIVYTIALTAIQA 692 Lambda K H 0.319 0.136 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1389 Number of extensions: 56 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 704 Length of database: 699 Length adjustment: 39 Effective length of query: 665 Effective length of database: 660 Effective search space: 438900 Effective search space used: 438900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 54 (25.4 bits)
Align candidate GFF734 PS417_03730 (phosphate acetyltransferase)
to HMM TIGR00651 (pta: phosphate acetyltransferase (EC 2.3.1.8))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR00651.hmm # target sequence database: /tmp/gapView.1551.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00651 [M=304] Accession: TIGR00651 Description: pta: phosphate acetyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 4.4e-118 380.1 0.1 5.9e-118 379.7 0.1 1.2 1 lcl|FitnessBrowser__WCS417:GFF734 PS417_03730 phosphate acetyltran Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__WCS417:GFF734 PS417_03730 phosphate acetyltransferase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 379.7 0.1 5.9e-118 5.9e-118 1 304 [] 389 689 .. 389 689 .. 0.98 Alignments for each domain: == domain 1 score: 379.7 bits; conditional E-value: 5.9e-118 TIGR00651 1 ivlPEgseervlkAaallaekkiaekvllvnkeeevkn.kakevnlklgkvvvedpdvskdiekyverlyekrkhk 75 ivlPEgse+ +++Aaa+++ ++ia++vll++ ++++++ +a++++l g +++ dpd+ +++yve ++ +rk k lcl|FitnessBrowser__WCS417:GFF734 389 IVLPEGSEPLTVQAAAICQARGIARCVLLAKPADVEAVaRAQGIELPEG-LEILDPDLI--RQRYVEPMVALRKSK 461 8************************************978999998765.788899999..89************* PP TIGR00651 76 GvtekeareqlrDevslaallvelgeadglvsGavsttaktlrpalqiiktlegvklvssvfimekeeevlvfaDC 151 ++ +a++ql+D+v++a+++++l+e+dglvsG ++ta+t+rpalq+ikt++g lvssvf+m +e+vlv++DC lcl|FitnessBrowser__WCS417:GFF734 462 SLNAPMAEQQLEDTVVIATMMLALDEVDGLVSGVIHSTANTIRPALQLIKTAPGCTLVSSVFFMLFPEQVLVYGDC 537 **************************************************************************** PP TIGR00651 152 avavdPnaeeLAeiAlqsaksakslgeeepkvallsystkgsgkgeevekvkeAvkilkekepdllldGelqfDaA 227 ++++P+a eLAeiAlqsa+sa ++g + p+va++sys++ s++geevekv+eA+ +++e+++ ll+dG+lq+DaA lcl|FitnessBrowser__WCS417:GFF734 538 VMNPHPSAAELAEIALQSADSAAAFG-ITPRVAMISYSSGDSASGEEVEKVREATLLAHEQQSSLLIDGPLQYDAA 612 **************************.************************************************* PP TIGR00651 228 lvekvaekkapesevagkanvfvFPdLdaGnigYkivqRladaeaiGPilqGlakPvnDLsRGasvedivnvviit 303 e+va++ ap+s+vagka+vfvFPdL++Gn++ k+vqR+ad ++GP+lqGl+kPvnDL RGa+v+div+++++t lcl|FitnessBrowser__WCS417:GFF734 613 ANENVARQLAPNSQVAGKATVFVFPDLNTGNTTHKAVQRSADCVSLGPMLQGLRKPVNDLPRGAQVDDIVYTIALT 688 ***************************************************************************9 PP TIGR00651 304 a 304 a lcl|FitnessBrowser__WCS417:GFF734 689 A 689 6 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (304 nodes) Target sequences: 1 (699 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00 # Mc/sec: 22.98 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory