Aligments for a candidate for exuT in Pseudomonas simiae WCS417

GapMind for catabolism of small carbon sources

Aligments for a candidate for exuT in Pseudomonas simiae WCS417

Align MFS transporter (characterized, see rationale)
to candidate GFF2889 PS417_14775 major facilitator transporter

Query= uniprot:A0A166QG26
         (467 letters)



>lcl|FitnessBrowser__WCS417:GFF2889 PS417_14775 major facilitator
           transporter
          Length = 467

 Score =  858 bits (2218), Expect = 0.0
 Identities = 416/467 (89%), Positives = 439/467 (94%)

Query: 1   MNLNEPINAQRIGQAVGNYRWTICALLFFATTVNYLDRQVLSLLAPDLSTQFGWSNSDYA 60
           MNLNEPINA R+GQAVGNYRWTICA+LFFATTVNYLDRQVLSLLAP LSTQFGWSN+DYA
Sbjct: 1   MNLNEPINAHRVGQAVGNYRWTICAMLFFATTVNYLDRQVLSLLAPQLSTQFGWSNTDYA 60

Query: 61  NIASVFQFVYAISMLFAGRVVDKIGTKTAYVVAICIWSTGAVMHAFAVPMGEGIGAVSSA 120
           NIA+VFQFVYAISMLFAGR VDKIGTK AYVVAI IWSTGA+MHAF+VPMGEGI A+S A
Sbjct: 61  NIAAVFQFVYAISMLFAGRFVDKIGTKAAYVVAIAIWSTGAIMHAFSVPMGEGIAAISGA 120

Query: 121 LGIAMIPVSIAGFMVSRAVLAIGEAGNFPIAIKATAEYFPKKERSFATGIFNSGANVGAI 180
           +G+A+IPVSIAGFM+SRAVLAIGEAGNFPIAIKATAEYFPKKERS ATGIFNSGANVGAI
Sbjct: 121 IGLAVIPVSIAGFMLSRAVLAIGEAGNFPIAIKATAEYFPKKERSLATGIFNSGANVGAI 180

Query: 181 LAPICVPLIASLWGWEAAFIVIGMLGFVWVGVWIALYEKPEQQKRLSAQELAYIRSDQVV 240
           LAPICVPLIA LWGWEAAFIVIG LGFVWV VWIALY+KPE+QKRLS +ELAYIRSDQ V
Sbjct: 181 LAPICVPLIAGLWGWEAAFIVIGGLGFVWVVVWIALYQKPEEQKRLSPEELAYIRSDQTV 240

Query: 241 PVVTRPVPGVADKKVSWFKLLTYRQTWAFAFGKFMTDGVWWFFLFWLPTYLSAQYGMKGQ 300
              T    G  +KKVSWFKLLTYRQTWAFAFGKFMTDGVWWFFLFWLPTYLSAQYGMKG 
Sbjct: 241 QPFTPAPAGAPEKKVSWFKLLTYRQTWAFAFGKFMTDGVWWFFLFWLPTYLSAQYGMKGA 300

Query: 301 AIVMPLAVLYSMTMIGSIGGGWFPSYFMSRGDAPYDGRMKAMLVIAFFPLLVLLAQPLGY 360
            IVMPLAVLYSMTM+GSIGGGWFPSYFM+RGDAPYDGRMKAMLVIA FPL+VLLAQPLGY
Sbjct: 301 DIVMPLAVLYSMTMVGSIGGGWFPSYFMARGDAPYDGRMKAMLVIALFPLVVLLAQPLGY 360

Query: 361 ISFWVPVLLIGVGASAHQAWSCNIFTTVSDMFPQKSIASVVGIGGLAGGLGGVVMTKIGG 420
           ISFWVPVLLIGVGASAHQAWSCNIFTTVSDMFPQK++ASVVGIGG+AGGLGGVVMTKIGG
Sbjct: 361 ISFWVPVLLIGVGASAHQAWSCNIFTTVSDMFPQKTVASVVGIGGMAGGLGGVVMTKIGG 420

Query: 421 WVIDHYKLIGDIHTGYMIMFAICALAYLVAWSVMKALVPRHKEITDL 467
           WV D+YK +GDIHTGYMIMFAICALAYLVAWSVMK LVPRHKEITDL
Sbjct: 421 WVFDYYKSVGDIHTGYMIMFAICALAYLVAWSVMKTLVPRHKEITDL 467


Lambda     K      H
   0.327    0.140    0.449 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1009
Number of extensions: 27
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 467
Length of database: 467
Length adjustment: 33
Effective length of query: 434
Effective length of database: 434
Effective search space:   188356
Effective search space used:   188356
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources