Align gluconate:H+ symporter (gntT) (characterized)
to candidate GFF4613 PS417_23605 permease DsdX
Query= reanno::Cup4G11:RR42_RS28835 (453 letters) >FitnessBrowser__WCS417:GFF4613 Length = 467 Score = 575 bits (1483), Expect = e-169 Identities = 287/453 (63%), Positives = 361/453 (79%), Gaps = 4/453 (0%) Query: 1 MGAVTGTTLLLYALIAVIALVVLIAKFKLNPFITLVVVSVLLGFAVGMPMGDIVKSFEAG 60 M A G LL YA IA+IAL+VLIA+++LNPFI + +VS+ L GMP I+ S+EAG Sbjct: 19 MAASPGFGLLAYAAIAIIALIVLIARYRLNPFIVITLVSIGLALMAGMPADTIMGSYEAG 78 Query: 61 VGGTLGHIALVVGLGTMLGKMMAESGGAERIARTLIDAFGEKNVHWAMVTIAFIVGLPVF 120 VG TLGHIALVV LGTMLGKMMAESGGAE++ARTLI+ FGE+N HWAMV IAF+VGLP+F Sbjct: 79 VGKTLGHIALVVALGTMLGKMMAESGGAEQVARTLINRFGERNAHWAMVCIAFLVGLPLF 138 Query: 121 FEVGFVLLVPIAFNVAKRTGTSMVLVGIPMVAGLSVVHGLIPPHPAALLAVTAYKADIGK 180 FEVGFVLLVPIAF VA+R G S+++VG+PMVAGLSVVH L+PPHPAA++AV AY A +G+ Sbjct: 139 FEVGFVLLVPIAFTVARRVGVSILMVGLPMVAGLSVVHALVPPHPAAMMAVLAYNASVGQ 198 Query: 181 TILYALIVGIPTAAIAGPLFAKLMTRYVTLPDVNPLAAQFTEEDEGVKASHELPGFGITL 240 T+LYA+++GIPTA IAGP++AK + + LP NPL QF + + + LP F +T+ Sbjct: 199 TVLYAILIGIPTAIIAGPVYAKFIVPRIHLPAENPLERQFIDREPRTR----LPSFTLTM 254 Query: 241 FTILLPVILMLIGSWADLITTPKTFANDFLKLIGNSVIALLIAALVSFYTFGKRRGFTRE 300 TILLPV+LM+IG WA++I++P + N FL IGNSVIALL+A LVSF+T G +GF RE Sbjct: 255 GTILLPVVLMMIGGWANVISSPGSAFNQFLLFIGNSVIALLVATLVSFWTLGIAQGFNRE 314 Query: 301 NILRFTNECVAPTAIITLVVGAGGGFGRVLRDSGISNAIVDVATGAHVSVLLLGWLVAVL 360 +IL+FTNEC+APTA ITL+VGAGGG R+L D+G++N I+ +A +S L++GWL A L Sbjct: 315 SILKFTNECLAPTASITLLVGAGGGLNRILVDAGVTNEILGLAHAFQLSPLVMGWLFAAL 374 Query: 361 IRIATGSATVAMTTAAGIVAPIAASVPGTRPELLVLTTGAGSLILSHVNDGGFWLVKEYF 420 +RIATGSATVAMTTA+G+VAP+A + PELLVL TGAGS+I SHVNDGGFWL+KEYF Sbjct: 375 MRIATGSATVAMTTASGVVAPVAMGLGYPHPELLVLATGAGSVIFSHVNDGGFWLIKEYF 434 Query: 421 NMTVAQTFKTWSVCETLISVIALLLTLALATVV 453 NMTV QTFKTW+V ETLISV+A LT L+ ++ Sbjct: 435 NMTVIQTFKTWTVLETLISVVAFGLTYGLSCIL 467 Lambda K H 0.326 0.141 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 775 Number of extensions: 52 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 453 Length of database: 467 Length adjustment: 33 Effective length of query: 420 Effective length of database: 434 Effective search space: 182280 Effective search space used: 182280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 51 (24.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory