GapMind for catabolism of small carbon sources

 

Finding step AO353_21725 for D-glucosamine (chitosamine) catabolism in Pseudomonas simiae WCS417

4 candidates for AO353_21725: glucosaminate ABC transporter, ATPase component

Score Gene Description Similar to Id. Cov. Bits Other hit Other id. Other bits
hi PS417_12110 ATP-binding protein ABC transporter for D-Glucosamine, putative ATPase component (characterized) 90% 99% 463.8 Probable ATP-binding component of ABC transporter, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR 52% 253.1
med PS417_18120 arginine ABC transporter ATP-binding protein ABC transporter for D-glucosamine, ATPase component (characterized) 53% 97% 279.6 NocP aka ATU6028 aka AGR_PTI_69, component of Nopaline porter 56% 283.9
med PS417_27400 amino acid ABC transporter ATP-binding protein ABC transporter for D-Glucosamine, putative ATPase component (characterized) 56% 96% 261.9 Amino acid ABC transporter ATP binding protein, component of Hydroxy L-proline uptake porter, HprABC 56% 262.3
med PS417_01500 amino acid transporter ABC transporter for D-glucosamine, ATPase component (characterized) 53% 94% 258.8 ABC transporter for L-Lysine, ATPase component 97% 486.9

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

Also see fitness data for the candidates

Definition of step AO353_21725

Or cluster all characterized AO353_21725 proteins

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory