Align protein-Npi-phosphohistidine-N-acetyl-D-glucosamine phosphotransferase (EC 2.7.1.193) (characterized)
to candidate GFF4494 PS417_23000 PTS N-acetyl-D-glucosamine transporter
Query= BRENDA::P09323 (648 letters) >FitnessBrowser__WCS417:GFF4494 Length = 568 Score = 483 bits (1243), Expect = e-140 Identities = 256/487 (52%), Positives = 335/487 (68%), Gaps = 18/487 (3%) Query: 8 QRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQAGGAIFDNLALIFAIGVASSWSKD 67 QRLGRAL LPIA+LP+A LLLR G DLLN+A + AG AIF NLALIFAIG+A +++D Sbjct: 10 QRLGRALMLPIAILPIAGLLLRLGDTDLLNIAVMHDAGQAIFANLALIFAIGIAVGFARD 69 Query: 68 SAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGIITGLVGGAAYNRWSDIKLPDFLSFF 127 + G A LAGA+GY V+ + ++ INMG+LAGI +GL+ G YNR+ DIKLP++L+FF Sbjct: 70 NNGTAGLAGAIGYLVMVSTLKVMDTSINMGMLAGIASGLMAGGLYNRFKDIKLPEYLAFF 129 Query: 128 GGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIHAGGEWIVSAGALGSGIFGFINRLLIPT 187 GG+RFVPI TGF + L IFG +WPP+QH I++ G ++ +G+LG+ +FG NRLLI T Sbjct: 130 GGRRFVPIVTGFSAVGLGVIFGLIWPPIQHGINSFGVLLMESGSLGAFVFGVFNRLLIVT 189 Query: 188 GLHQVLNTIAWFQIGEFTN-AAGTVFHGDINRFYAGDGTAGMFMSGFFPIMMFGLPGAAL 246 GLH +LN +AWF G FT+ G V GD+ R++AGD G FM+G FP+M+FGLP A L Sbjct: 190 GLHHILNNMAWFVFGSFTDPVTGAVVTGDLTRYFAGDPKGGQFMTGMFPVMLFGLPAACL 249 Query: 247 AMYFAAPKERRPMVGGMLLSVAVTAFLTGVTEPLEFLFMFLAPLLYLLHALLTGISLFVA 306 AMY A ERR ++GG+ LS+A+T+FLTGVTEP+EF FMFLAP LYL+HA+LTG+S+ V Sbjct: 250 AMYRNALPERRKVMGGIFLSMALTSFLTGVTEPIEFAFMFLAPFLYLIHAVLTGLSMAVT 309 Query: 307 TLLGIHAGFSFSAGAIDYALMYNLPAASQNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKT 366 +L IH GF+FS G ID L + S N W++ +G+ + IY+ VF+ IR FNLKT Sbjct: 310 NMLNIHLGFTFSGGFIDMVLGW---GKSTNGWLVFPVGLAYALIYYSVFNYCIRHFNLKT 366 Query: 367 PGREDKEDEIVTEEANSNTEEGLTQLATNYIAAVGGTDNLKAIDACITRLRLTVADSARV 426 PGRED ++V EA S+ Q AT YI A+GG NL ++ AC TRLRL + D + Sbjct: 367 PGRED--TQVVQAEAMSD-----NQRATAYIQALGGAQNLLSVGACTTRLRLDMVDRNKA 419 Query: 427 NDTMCKRLGASGVVKL-NKQTIQVIVGAKAESIGDAMKKVVARGPVAAASAEATPATAAP 485 D K LGA VV+ N ++QV+VG A+SI D + ++ G VAAA P AP Sbjct: 420 VDAELKALGAMAVVRPGNGGSLQVVVGPMADSIADEI-RLAMPGFVAAA-----PVVVAP 473 Query: 486 VAKPQAV 492 V KP AV Sbjct: 474 VEKPVAV 480 Score = 48.9 bits (115), Expect = 6e-10 Identities = 24/86 (27%), Positives = 51/86 (59%), Gaps = 1/86 (1%) Query: 391 QLATNYIAAVGGTDNLKAIDA-CITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQV 449 Q A ++ A+GG N++ ++A +TRLR+ + D + +++ LG GV +L + Sbjct: 483 QEAEKWLNALGGRGNVRQLEAVAMTRLRVELGDDSGLSEADLTALGCQGVSQLESGVWHL 542 Query: 450 IVGAKAESIGDAMKKVVARGPVAAAS 475 ++G KA +G+A++++V + A++ Sbjct: 543 LIGDKATGLGEALERLVGAQQIGASA 568 Lambda K H 0.324 0.139 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 987 Number of extensions: 59 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 2 Length of query: 648 Length of database: 568 Length adjustment: 37 Effective length of query: 611 Effective length of database: 531 Effective search space: 324441 Effective search space used: 324441 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory