Align tartronate semialdehyde reductase 2 (characterized)
to candidate GFF1730 PS417_08800 tartronate semialdehyde reductase
Query= ecocyc::G6278-MONOMER (292 letters) >FitnessBrowser__WCS417:GFF1730 Length = 296 Score = 370 bits (950), Expect = e-107 Identities = 186/291 (63%), Positives = 226/291 (77%), Gaps = 1/291 (0%) Query: 2 KLGFIGLGIMGTPMAINLARAGHQLHVTTI-GPVADELLSLGAVSVETARQVTEASDIIF 60 K+GFIG GIMG PMA NL +AGHQL ++ G A L+ GAV++ +QV + ++ I Sbjct: 3 KIGFIGTGIMGQPMAANLQKAGHQLFLSEHHGKAAQALVDAGAVALANPQQVAQEAEFII 62 Query: 61 IMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKRFARQVNELGGDYLDAPVS 120 +MVPDTPQV++VLF ++G K ++DMSSISP TK FA ++NE G YLDAPVS Sbjct: 63 VMVPDTPQVDDVLFRKDGVAAGLSPNKVVIDMSSISPTATKAFAAKINETGAQYLDAPVS 122 Query: 121 GGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVGGNGDGQTCKVANQIIVALNI 180 GGE+GA+ GTLSIM+GG+ FER PLF+ +GKNITLVGGNGDGQT KVANQIIVALNI Sbjct: 123 GGEVGAKAGTLSIMIGGEPQTFERALPLFQAMGKNITLVGGNGDGQTAKVANQIIVALNI 182 Query: 181 EAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHGERMIKRTFNPGFKIALHQKDLN 240 +AV+EALLFASK GADP +VR+ALMGGFASS+ILEVHGERMIK TF+PGF+I LHQKDLN Sbjct: 183 QAVAEALLFASKNGADPAKVREALMGGFASSKILEVHGERMIKGTFDPGFRINLHQKDLN 242 Query: 241 LALQSAKALALNLPNTATCQELFNTCAANGGSQLDHSALVQALELMANHKL 291 LAL AK L +NLPNTA Q++F+TC A GG DHSAL++ LE MAN + Sbjct: 243 LALAGAKELGINLPNTAGTQQVFSTCTAIGGGNWDHSALIKGLEHMANFSI 293 Lambda K H 0.318 0.135 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 338 Number of extensions: 8 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 292 Length of database: 296 Length adjustment: 26 Effective length of query: 266 Effective length of database: 270 Effective search space: 71820 Effective search space used: 71820 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 48 (23.1 bits)
Align candidate GFF1730 PS417_08800 (tartronate semialdehyde reductase)
to HMM TIGR01505 (2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01505.hmm # target sequence database: /tmp/gapView.18344.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01505 [M=291] Accession: TIGR01505 Description: tartro_sem_red: 2-hydroxy-3-oxopropionate reductase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.7e-121 390.9 7.9 1.9e-121 390.7 7.9 1.0 1 lcl|FitnessBrowser__WCS417:GFF1730 PS417_08800 tartronate semialdeh Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__WCS417:GFF1730 PS417_08800 tartronate semialdehyde reductase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 390.7 7.9 1.9e-121 1.9e-121 1 289 [. 3 291 .. 3 293 .. 0.99 Alignments for each domain: == domain 1 score: 390.7 bits; conditional E-value: 1.9e-121 TIGR01505 1 kvgfiGlGimGkPmsknllkaGyqlvvatleqealdellaaGaesaetakevvedadvivtmvPdsPqveevalG 75 k+gfiG GimG+Pm+ nl kaG+ql + + +a++ l+ aGa++ ++v+++a+ i++mvPd+Pqv++v++ lcl|FitnessBrowser__WCS417:GFF1730 3 KIGFIGTGIMGQPMAANLQKAGHQLFLSEHHGKAAQALVDAGAVALANPQQVAQEAEFIIVMVPDTPQVDDVLFR 77 89************************************************************************* PP TIGR01505 76 enGileaakkGkvlvdmssiaPleskelakavkekGidvldaPvsGGeagaiegtlsimvGGdkavfdkvkplle 150 ++G+ + + kv++dmssi+P ++k +a +++e G ++ldaPvsGGe+ga+ gtlsim+GG+ f+++ pl++ lcl|FitnessBrowser__WCS417:GFF1730 78 KDGVAAGLSPNKVVIDMSSISPTATKAFAAKINETGAQYLDAPVSGGEVGAKAGTLSIMIGGEPQTFERALPLFQ 152 *************************************************************************** PP TIGR01505 151 algksivlvGenGaGqtvkvanqvivalnieavsealvlaekaGvdpkavlqalrGGlagstvleakkerlldrd 225 a+gk+i+lvG+nG+Gqt+kvanq+ivalni+av+eal++a+k G+dp +v++al+GG+a+s++le+++er+++ + lcl|FitnessBrowser__WCS417:GFF1730 153 AMGKNITLVGGNGDGQTAKVANQIIVALNIQAVAEALLFASKNGADPAKVREALMGGFASSKILEVHGERMIKGT 227 *************************************************************************** PP TIGR01505 226 fkPGfridlhqkdlalaldaakavgaalPvtavvaellaalradGdgtldhsalvraleklakd 289 f+PGfri+lhqkdl+lal ak++g++lP+ta +++++++ a G+g+ dhsal++ le++a+ lcl|FitnessBrowser__WCS417:GFF1730 228 FDPGFRINLHQKDLNLALAGAKELGINLPNTAGTQQVFSTCTAIGGGNWDHSALIKGLEHMANF 291 **************************************************************85 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (291 nodes) Target sequences: 1 (296 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 8.50 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory