Align aspartate ammonia-lyase (EC 4.3.1.1) (characterized)
to candidate GFF5496 PS417_28130 aspartate ammonia-lyase
Query= BRENDA::Q9HTD7 (474 letters) >FitnessBrowser__WCS417:GFF5496 Length = 474 Score = 827 bits (2137), Expect = 0.0 Identities = 427/474 (90%), Positives = 443/474 (93%) Query: 1 MSPVASSRIEKDLLGTLEVPADAYYGIQTLRAVNNFRLSGVPLSHYPKLVVALAMVKQAA 60 MS AS R EKDLLG LEVPA AYYGIQTLRAVNNFRLSGVP+SHYPKLVV LAMVKQAA Sbjct: 1 MSSAASFRTEKDLLGVLEVPAQAYYGIQTLRAVNNFRLSGVPISHYPKLVVGLAMVKQAA 60 Query: 61 ADANRQLGHLPEDKHAAISEACARLIRGDFHEQFVVDMIQGGAGTSTNMNANEVIANIAL 120 ADANR+LG L E KHAAISEACARLIRGDFHE+FVVDMIQGGAGTSTNMNANEVIANIAL Sbjct: 61 ADANRELGQLSEAKHAAISEACARLIRGDFHEEFVVDMIQGGAGTSTNMNANEVIANIAL 120 Query: 121 EAMGHTKGEYKYLHPNNDVNMAQSTNDAYPTAIRLGLLLGHDTLLASLDSLIQAFAAKGV 180 EAMGH KGEY+YLHPNNDVNMAQSTNDAYPTAIRLGLLLGHD LLASLDSLIQAFAAKG Sbjct: 121 EAMGHNKGEYQYLHPNNDVNMAQSTNDAYPTAIRLGLLLGHDALLASLDSLIQAFAAKGE 180 Query: 181 EFAGVLKMGRTQLQDAVPMTLGQEFHAFATTLGEDLDRLRRLAPELLTEVNLGGTAIGTG 240 EF+ VLKMGRTQLQDAVPMTLGQEF AFATTLGEDL RL+ LAPELLTEVNLGGTAIGTG Sbjct: 181 EFSHVLKMGRTQLQDAVPMTLGQEFRAFATTLGEDLARLKTLAPELLTEVNLGGTAIGTG 240 Query: 241 INADPGYQKLAVERLAAISGQPLKPAADLIEATSDMGAFVLFSGMLKRTAVKLSKICNDL 300 INADP YQ LAV+RLA ISGQP+ PAADLIEATSDMGAFVLFSGMLKRTAVKLSKICNDL Sbjct: 241 INADPRYQALAVQRLAVISGQPVVPAADLIEATSDMGAFVLFSGMLKRTAVKLSKICNDL 300 Query: 301 RLLSSGPRTGINEINLPPRQPGSSIMPGKVNPVIPEAVNQVAFEVIGNDLALTLAAEGGQ 360 RLLSSGPRTGINEINLP RQPGSSIMPGKVNPVIPEAVNQVAF+VIGNDLALT+AAEGGQ Sbjct: 301 RLLSSGPRTGINEINLPARQPGSSIMPGKVNPVIPEAVNQVAFQVIGNDLALTMAAEGGQ 360 Query: 361 LQLNVMEPLIAYKIFDSIRLLQRAMDMLREHCITGITANVERCHELVEHSIGLVTALNPY 420 LQLNVMEPLIA+KIFDSIRLLQRAMDMLREHCI GITAN RC ELVEHSIGLVTALNPY Sbjct: 361 LQLNVMEPLIAFKIFDSIRLLQRAMDMLREHCIVGITANEARCRELVEHSIGLVTALNPY 420 Query: 421 IGYENSTRIAKTALESGRGVLELVREEKLLDEATLADILLPENMIAPRLIPLRA 474 IGYEN+TRIA+ ALESGRGVLELVREE LLD+A L DIL PENMIAPRL+PL+A Sbjct: 421 IGYENATRIARIALESGRGVLELVREEGLLDDAMLDDILRPENMIAPRLVPLKA 474 Lambda K H 0.318 0.135 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 812 Number of extensions: 21 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 474 Length of database: 474 Length adjustment: 33 Effective length of query: 441 Effective length of database: 441 Effective search space: 194481 Effective search space used: 194481 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
Align candidate GFF5496 PS417_28130 (aspartate ammonia-lyase)
to HMM TIGR00839 (aspA: aspartate ammonia-lyase (EC 4.3.1.1))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR00839.hmm # target sequence database: /tmp/gapView.18320.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00839 [M=468] Accession: TIGR00839 Description: aspA: aspartate ammonia-lyase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1e-210 686.5 0.1 1.2e-210 686.3 0.1 1.0 1 lcl|FitnessBrowser__WCS417:GFF5496 PS417_28130 aspartate ammonia-ly Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__WCS417:GFF5496 PS417_28130 aspartate ammonia-lyase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 686.3 0.1 1.2e-210 1.2e-210 1 460 [. 8 468 .. 8 473 .. 0.99 Alignments for each domain: == domain 1 score: 686.3 bits; conditional E-value: 1.2e-210 TIGR00839 1 riekdllGekeveaevyyGiqtlrasenfaisnekisdvpefvkalvlvkkaaalanvelkaidekiakaivaac 75 r ekdllG ev+a++yyGiqtlra++nf +s++ is++p++v +l++vk+aaa an el++++e ai +ac lcl|FitnessBrowser__WCS417:GFF5496 8 RTEKDLLGVLEVPAQAYYGIQTLRAVNNFRLSGVPISHYPKLVVGLAMVKQAAADANRELGQLSEAKHAAISEAC 82 89************************************************************************* PP TIGR00839 76 deileGkyldqfivdviqGGaGtsvnmntnevianlalellGhkkGeyqflnpndhvnksqstndayptalkiav 150 ++ G ++++f+vd+iqGGaGts+nmn+nevian+ale +Gh+kGeyq+l+pn+ vn++qstndaypta+++ + lcl|FitnessBrowser__WCS417:GFF5496 83 ARLIRGDFHEEFVVDMIQGGAGTSTNMNANEVIANIALEAMGHNKGEYQYLHPNNDVNMAQSTNDAYPTAIRLGL 157 *************************************************************************** PP TIGR00839 151 yesleklvdkiealrdafeqkakefadvlkmGrtqlqdavpltlGqefeayalllerdvkrikr.trelllevnl 224 + l++++++l +af+ k +ef +vlkmGrtqlqdavp+tlGqef+a+a l +d+ r+k + ell evnl lcl|FitnessBrowser__WCS417:GFF5496 158 LLGHDALLASLDSLIQAFAAKGEEFSHVLKMGRTQLQDAVPMTLGQEFRAFATTLGEDLARLKTlAPELLTEVNL 232 **************************************************************98355899***** PP TIGR00839 225 GataiGtGlnadkeysklvvkklaevtGlplvpaenlieatsdtgayvevsgalkriavklskvcndlrllssGp 299 G+taiGtG+nad+ y+ l+v++la ++G p+vpa +lieatsd ga+v sg lkr+avklsk+cndlrllssGp lcl|FitnessBrowser__WCS417:GFF5496 233 GGTAIGTGINADPRYQALAVQRLAVISGQPVVPAADLIEATSDMGAFVLFSGMLKRTAVKLSKICNDLRLLSSGP 307 *************************************************************************** PP TIGR00839 300 raGlneinlpelqaGssimpakvnpvvpevvnqvcfkviGndttvtlaaeaGqlqlnvlepviafallesisilt 374 r+G+neinlp++q+Gssimp+kvnpv+pe vnqv+f+viGnd ++t+aae+Gqlqlnv+ep+iaf++++si +l+ lcl|FitnessBrowser__WCS417:GFF5496 308 RTGINEINLPARQPGSSIMPGKVNPVIPEAVNQVAFQVIGNDLALTMAAEGGQLQLNVMEPLIAFKIFDSIRLLQ 382 *************************************************************************** PP TIGR00839 375 naiesltdkcveGitanekicedyvfnsiGivtalnpfiGyekaalvakeaiktgksvrdvvlekdllteeeldd 449 +a+ l+++c+ Gitane +c++ v++siG+vtalnp+iGye+a ++a++a+++g++v ++v e++ll ++ ldd lcl|FitnessBrowser__WCS417:GFF5496 383 RAMDMLREHCIVGITANEARCRELVEHSIGLVTALNPYIGYENATRIARIALESGRGVLELVREEGLLDDAMLDD 457 *************************************************************************** PP TIGR00839 450 ilsvenllkpa 460 il++en++ p lcl|FitnessBrowser__WCS417:GFF5496 458 ILRPENMIAPR 468 ********995 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (468 nodes) Target sequences: 1 (474 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.03 # Mc/sec: 7.02 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory