GapMind for catabolism of small carbon sources

 

Finding step gltJ for L-glutamate catabolism in Pseudomonas simiae WCS417

5 candidates for gltJ: L-glutamate ABC transporter, permease component 1 (gltJ/aatQ)

Score Gene Description Similar to Id. Cov. Bits Other hit Other id. Other bits
hi PS417_05555 amino acid ABC transporter permease ABC transporter for L-aspartate, L-asparagine, L-glutamate, and L-glutamine, permease component 2 (characterized) 96% 100% 488.4 NatG, component of Acidic and neutral amino acid uptake transporter NatFGH/BgtA. BgtA is shared with BgtAB 35% 125.6
lo PS417_01810 ABC transporter permease Amino acid ABC transporter membrane protein, component of Amino acid transporter, AatJMQP. Probably transports L-glutamic acid, D-glutamine acid, L-glutamine and N-acetyl L-glutamic acid (Johnson et al. 2008). Very similar to 3.A.1.3.19 of P. putida (characterized) 33% 87% 131.7 Basic amino acid uptake transporter, BgtAB 49% 177.2
lo PS417_05550 amino acid ABC transporter permease Amino acid ABC transporter membrane protein, component of Amino acid transporter, AatJMQP. Probably transports L-glutamic acid, D-glutamine acid, L-glutamine and N-acetyl L-glutamic acid (Johnson et al. 2008). Very similar to 3.A.1.3.19 of P. putida (characterized) 33% 85% 129 ABC transporter for L-Asparagine and possibly other L-amino acids, permease component 2 87% 396.7
lo PS417_18125 polar amino acid ABC transporter permease PP1070, component of Acidic amino acid uptake porter, AatJMQP (characterized) 35% 90% 125.2 BgtB aka GLNH aka SLL1270, component of Arginine/lysine/histidine/glutamine porter 45% 168.7
lo PS417_01100 amino acid ABC transporter permease Glutamate/aspartate import permease protein GltJ (characterized) 32% 93% 104.8 L-cystine transport system permease protein YecS 73% 320.1

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

Also see fitness data for the candidates

Definition of step gltJ

Or cluster all characterized gltJ proteins

This GapMind analysis is from Aug 02 2021. The underlying query database was built on Aug 02 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory