GapMind for catabolism of small carbon sources

 

Finding step glpT for glycerol catabolism in Pseudomonas simiae WCS417

3 candidates for glpT: glycerol ABC transporter, ATPase component 2 (GlpT)

Score Gene Description Similar to Id. Cov. Bits Other hit Other id. Other bits
hi PS417_16235 ABC transporter ATP-binding protein GlpT, component of Glycerol uptake porter, GlpSTPQV (characterized) 55% 98% 399.1 MalK; aka Sugar ABC transporter, ATP-binding protein, component of The maltose, maltotriose, mannotetraose (MalE1)/maltose, maltotriose, trehalose (MalE2) porter (Nanavati et al., 2005). For MalG1 (823aas) and MalG2 (833aas), the C-terminal transmembrane domain with 6 putative TMSs is preceded by a single N-terminal TMS and a large (600 residue) hydrophilic region showing sequence similarity to MLP1 and 2 (9.A.14; e-12 & e-7) as well as other proteins 36% 224.9
lo PS417_12700 ABC transporter ATP-binding protein GlpT, component of Glycerol uptake porter, GlpSTPQV (characterized) 39% 80% 208.4 ABC transporter for D-Mannitol, D-Mannose, and D-Sorbitol, ATPase component 100% 715.7
lo PS417_22130 sugar ABC transporter ATPase GlpT, component of Glycerol uptake porter, GlpSTPQV (characterized) 35% 97% 206.8 ABC transporter for D-Glucose-6-Phosphate, ATPase component 100% 757.3

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

Also see fitness data for the candidates

Definition of step glpT

Or cluster all characterized glpT proteins

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory