Align histidine ABC transporter, periplasmic histidine-binding protein HisJ (characterized)
to candidate GFF4245 PS417_21745 ABC transporter substrate-binding protein
Query= CharProtDB::CH_014295 (260 letters) >FitnessBrowser__WCS417:GFF4245 Length = 261 Score = 229 bits (583), Expect = 6e-65 Identities = 114/259 (44%), Positives = 163/259 (62%), Gaps = 2/259 (0%) Query: 1 MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNSQGELVGFDIDLAKELC 60 MKKLVL +L L+ S A P ++IG + Y PF SK G +VGFD D+ LC Sbjct: 1 MKKLVLLGALALSVLSMQAFAEGKP--LKIGIEAAYPPFASKAPDGSIVGFDYDIGNALC 58 Query: 61 KRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQEIAFTDKLYAADSRLVVAK 120 + + +CT+VE D LIP+LK +KIDAI+SS+SIT+ R++ + FT++ Y +RLV+ + Sbjct: 59 EEMKVKCTWVEQEFDGLIPALKVRKIDAILSSMSITDDRKKSVDFTNRYYLTPARLVLKE 118 Query: 121 NSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIVSYQGQDNIYSDLTAGRIDAA 180 + + +++ LKGK++GV +G+ + F E APKG IV Y Q+ IY D+ AGR+D Sbjct: 119 GTAVSDSLDELKGKKIGVQRGSIHDRFAKEVLAPKGATIVPYSSQNEIYLDVEAGRLDGT 178 Query: 181 FQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTGMGLRKEDNELREALNKAFAEMR 240 D EGFL P GK Y F GP+ D K FG G G+ +RK D E + +N A A +R Sbjct: 179 VADATLLQEGFLDTPAGKGYAFTGPAFTDAKYFGDGIGIAVRKGDKENLDRINAAIAAIR 238 Query: 241 ADGTYEKLAKKYFDFDVYG 259 A+G Y+++ KKYF+FD+YG Sbjct: 239 ANGKYKEIEKKYFNFDIYG 257 Lambda K H 0.315 0.133 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 185 Number of extensions: 4 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 260 Length of database: 261 Length adjustment: 25 Effective length of query: 235 Effective length of database: 236 Effective search space: 55460 Effective search space used: 55460 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory