Align Amino acid (Lysine/arginine/ornithine/histidine/octopine) ABC transporter periplasmic binding protein, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized)
to candidate GFF5094 PS417_26100 amino acid ABC transporter
Query= TCDB::Q9HU31 (250 letters) >FitnessBrowser__WCS417:GFF5094 Length = 253 Score = 207 bits (526), Expect = 2e-58 Identities = 114/247 (46%), Positives = 155/247 (62%), Gaps = 4/247 (1%) Query: 7 ILLAAAATLAFALDASAADKLRIGTEGAYPPFNGIDASGQAVGFDLDIGKALCAKMKTEC 66 I A L A+ A+ LRI +EGAYPPFN +D++ + GFD+DI ALC +MK C Sbjct: 8 ITTATLWLLCTVAGAAHAETLRIASEGAYPPFNYVDSNNRLHGFDIDIANALCERMKVNC 67 Query: 67 EVVTSDWDGIIPALNAKKFDFIVASMSITDERKQAVDFTDPYYTNKLQFVAPKSVDFKTD 126 VT DW+G+IPAL AKK+D IVASM++T+ERK+ + FT+ YY L Sbjct: 68 TFVTQDWEGMIPALLAKKYDAIVASMNVTEERKKKIAFTNRYYRTPLSVAVAHDSGISDA 127 Query: 127 KDSLKGKVIGAQRATIAGTWLEDNMADV-VTIKLYDTQENAYLDLSSGRLDGVLADKFVQ 185 + + KG +GAQ + + E++ A+ T+KLY +Q+ A DL++GRLDGV+ADK+ Sbjct: 128 QTNFKGLTVGAQSYSTQAIYAEEHYAEAGATVKLYPSQDEAASDLAAGRLDGVIADKYPL 187 Query: 186 YDWLKSDAGKE-FEFKGEPVFDNDKIGIAVRKGD-PLREKLNAALKEIVADGTYKKINDK 243 W+ + AGK+ F G+ N IAVR+GD LRE+LN AL EIVADGTYK I+ + Sbjct: 188 LAWV-AGAGKDCCTFLGDIDGTNTDAAIAVRQGDVELRERLNKALDEIVADGTYKNISRR 246 Query: 244 YFPFSIY 250 YF F IY Sbjct: 247 YFAFDIY 253 Lambda K H 0.317 0.135 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 189 Number of extensions: 8 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 250 Length of database: 253 Length adjustment: 24 Effective length of query: 226 Effective length of database: 229 Effective search space: 51754 Effective search space used: 51754 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory