Alignments for a candidate for fadA in Pseudomonas simiae WCS417

GapMind for catabolism of small carbon sources

Alignments for a candidate for fadA in Pseudomonas simiae WCS417

Align acetyl-CoA:acetyl-CoA C-acetyltransferase / acetyl-CoA:propanoyl-CoA 2-C-acetyltransferase (EC 2.3.1.9; EC 2.3.1.16) (characterized)
to candidate GFF2716 PS417_13855 acetyl-CoA acetyltransferase

Query= reanno::pseudo3_N2E3:AO353_25685
         (397 letters)



>FitnessBrowser__WCS417:GFF2716
          Length = 394

 Score =  686 bits (1769), Expect = 0.0
 Identities = 346/392 (88%), Positives = 370/392 (94%)

Query: 6   DPIVIVSAVRTPMGGFQGELKSLSAPQLGAAAIRAAVERAGVAADAVEEVLFGCVLSAGL 65
           DPIVIVSAVRTPMGGFQG+LK L+APQLG+AAIRAAVERAG+A DAV+EVLFGCVL AGL
Sbjct: 3   DPIVIVSAVRTPMGGFQGDLKGLTAPQLGSAAIRAAVERAGIATDAVDEVLFGCVLPAGL 62

Query: 66  GQAPARQAALGAGLDKSTRCTTLNKMCGSGMEAAILAHDMLLAGSADVVVAGGMESMSNA 125
           GQAPARQAALGAGLDK+TRCTTLNKMCGSGMEAAILAHD LLAGS DVV+AGGMESMSNA
Sbjct: 63  GQAPARQAALGAGLDKATRCTTLNKMCGSGMEAAILAHDSLLAGSVDVVIAGGMESMSNA 122

Query: 126 PYLLDRARSGYRMGHGKVLDHMFLDGLEDAYDKGRLMGTFAEDCAEANGFTREAQDEFAI 185
           PYLLDRARSGYRMGH KVLDHMFLDGLEDAYDKGRLMGTFAEDCA  NGFTREAQD FAI
Sbjct: 123 PYLLDRARSGYRMGHSKVLDHMFLDGLEDAYDKGRLMGTFAEDCALHNGFTREAQDAFAI 182

Query: 186 ASTTRAQQAIKDGSFNAEIVPLQVIVGKEQKLITDDEQPPKAKLDKIASLKPAFRDGGTV 245
           AS TRAQ+AI  G+F AEIVP+QV VGKEQK I  DEQPPKAKLDKI+SLK AFRDGGTV
Sbjct: 183 ASLTRAQEAISHGNFAAEIVPVQVTVGKEQKTILHDEQPPKAKLDKISSLKAAFRDGGTV 242

Query: 246 TAANSSSISDGAAALLLMRRSEAEKRGLKPLAVIHGHAAFADTPGLFPVAPVGAIKKLLK 305
           TAAN+SSISDGAAALLLMR+SEA+KRGLKPLAVIHGHAAFAD PGLFPVAPVGAI+KL+ 
Sbjct: 243 TAANASSISDGAAALLLMRQSEAQKRGLKPLAVIHGHAAFADEPGLFPVAPVGAIRKLMT 302

Query: 306 KTGWSLDEVELFEVNEAFAVVSLVTMTKLEIPHSKVNVHGGACALGHPIGASGARILVTL 365
           KTGW++ EV+LFE+NEAFAVVSLVTM+KL+IPH+KVN+HGGACALGHPIGASGARILVTL
Sbjct: 303 KTGWNVGEVDLFEINEAFAVVSLVTMSKLDIPHAKVNIHGGACALGHPIGASGARILVTL 362

Query: 366 LSALRQKGLKRGVAAICIGGGEATAMAVECLY 397
           LSALRQ GLKRGVAAICIGGGEATAMAVECLY
Sbjct: 363 LSALRQNGLKRGVAAICIGGGEATAMAVECLY 394


Lambda     K      H
   0.318    0.133    0.378 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 593
Number of extensions: 10
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 397
Length of database: 394
Length adjustment: 31
Effective length of query: 366
Effective length of database: 363
Effective search space:   132858
Effective search space used:   132858
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources