Align dihydrolipoyl dehydrogenase; EC 1.8.1.4 (characterized)
to candidate GFF1751 PS417_08905 dihydrolipoamide dehydrogenase
Query= CharProtDB::CH_015561 (478 letters) >FitnessBrowser__WCS417:GFF1751 Length = 478 Score = 845 bits (2184), Expect = 0.0 Identities = 429/478 (89%), Positives = 450/478 (94%) Query: 1 MTQKFDVVVIGAGPGGYVAAIKAAQLGLKTACIEKYTDAEGKLALGGTCLNVGCIPSKAL 60 MTQKFDVVVIGAGPGGYVAAIKAAQLGL TACIEKYTD EGKLALGGTCLNVGCIPSKAL Sbjct: 1 MTQKFDVVVIGAGPGGYVAAIKAAQLGLSTACIEKYTDKEGKLALGGTCLNVGCIPSKAL 60 Query: 61 LDSSWKYKEAKESFNVHGISTGEVKMDVAAMVGRKAGIVKNLTGGVATLFKANGVTSIQG 120 LDSSWK+ EA++ F +HGI V MDV AMVGRKA IVK LT GVATLFKANGVTS+QG Sbjct: 61 LDSSWKFHEAQDGFAIHGIHHAGVTMDVPAMVGRKANIVKGLTSGVATLFKANGVTSLQG 120 Query: 121 HGKLLAGKKVEVTKADGTTEVIEAENVILASGSRPIDIPPAPVDQNVIVDSTGALEFQAV 180 HGKLLAGKKVE+TK DG+TE+IEAENVILA GSRPIDIPPAPVDQNVIVDSTGALEFQAV Sbjct: 121 HGKLLAGKKVEITKPDGSTEIIEAENVILAPGSRPIDIPPAPVDQNVIVDSTGALEFQAV 180 Query: 181 PKRLGVIGAGVIGLELGSVWARLGAEVTVLEALDTFLMAADTAVSKEAQKTLTKQGLDIK 240 PKRLGVIGAGVIGLELGSVW+RLG+EVTVLEALDTFL+AADTAVSKEA KTLTKQGLDIK Sbjct: 181 PKRLGVIGAGVIGLELGSVWSRLGSEVTVLEALDTFLLAADTAVSKEALKTLTKQGLDIK 240 Query: 241 LGARVTGSKVNGNEVEVTYTNAEGEQKITFDKLIVAVGRRPVTTDLLAADSGVTIDERGY 300 LGARVTGSKVNG EV V YT+ +GE+ ITFDKLIVAVGRRPVTTDLLAADSGVT+DERGY Sbjct: 241 LGARVTGSKVNGEEVVVNYTDKDGEKTITFDKLIVAVGRRPVTTDLLAADSGVTLDERGY 300 Query: 301 IFVDDYCATSVPGVYAIGDVVRGMMLAHKASEEGIMVVERIKGHKAQMNYDLIPSVIYTH 360 + VDD+CAT+VPGVYAIGDVVRGMMLAHKASEEGIMVVERIKGHK QMNYDLIPSVIYTH Sbjct: 301 VHVDDHCATTVPGVYAIGDVVRGMMLAHKASEEGIMVVERIKGHKTQMNYDLIPSVIYTH 360 Query: 361 PEIAWVGKTEQALKAEGVEVNVGTFPFAASGRAMAANDTGGFVKVIADAKTDRVLGVHVI 420 PEIAWVGK EQ LKAEGVEVNVGTFPFAASGRAMAANDTGGFVKVIADAKTDRVLGVHVI Sbjct: 361 PEIAWVGKNEQQLKAEGVEVNVGTFPFAASGRAMAANDTGGFVKVIADAKTDRVLGVHVI 420 Query: 421 GPSAAELVQQGAIAMEFGTSAEDLGMMVFSHPTLSEALHEAALAVNGGAIHVANRKKR 478 GP AAELVQQGAI MEFGTSAED+GMMVFSHPTLSEALHEAALAVNGGAIH+AN+KKR Sbjct: 421 GPGAAELVQQGAIGMEFGTSAEDIGMMVFSHPTLSEALHEAALAVNGGAIHIANKKKR 478 Lambda K H 0.316 0.133 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 892 Number of extensions: 19 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 478 Length of database: 478 Length adjustment: 34 Effective length of query: 444 Effective length of database: 444 Effective search space: 197136 Effective search space used: 197136 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 51 (24.3 bits)
Align candidate GFF1751 PS417_08905 (dihydrolipoamide dehydrogenase)
to HMM TIGR01350 (lpdA: dihydrolipoyl dehydrogenase (EC 1.8.1.4))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01350.hmm # target sequence database: /tmp/gapView.8335.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01350 [M=461] Accession: TIGR01350 Description: lipoamide_DH: dihydrolipoyl dehydrogenase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.4e-164 532.8 10.1 3.9e-164 532.6 10.1 1.0 1 lcl|FitnessBrowser__WCS417:GFF1751 PS417_08905 dihydrolipoamide deh Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__WCS417:GFF1751 PS417_08905 dihydrolipoamide dehydrogenase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 532.6 10.1 3.9e-164 3.9e-164 1 461 [] 4 472 .. 4 472 .. 0.96 Alignments for each domain: == domain 1 score: 532.6 bits; conditional E-value: 3.9e-164 TIGR01350 1 eydvvviGgGpgGYvaAiraaqlglkvalvek..ek.....lGGtClnvGCiPtKalLksaevveelke.akelg 67 ++dvvviG+GpgGYvaAi+aaqlgl++a++ek +k lGGtClnvGCiP+KalL s+ ++e+++ ++ +g lcl|FitnessBrowser__WCS417:GFF1751 4 KFDVVVIGAGPGGYVAAIKAAQLGLSTACIEKytDKegklaLGGTCLNVGCIPSKALLDSSWKFHEAQDgFAIHG 78 59*****************************95421344449********************************* PP TIGR01350 68 ievenvkldlekllerkekvvkklvgGvkaLlkknkvevikGeaklldkkevevkkekke.kkleakniiiAtGs 141 i+ ++v++d+ +++ rk ++vk l++Gv++L+k n+v++++G++kll k+ve++k +++ + +ea+n+i+A Gs lcl|FitnessBrowser__WCS417:GFF1751 79 IHHAGVTMDVPAMVGRKANIVKGLTSGVATLFKANGVTSLQGHGKLLAGKKVEITKPDGStEIIEAENVILAPGS 153 *******************************************************99887799************ PP TIGR01350 142 eprelplkleedekvvitseealelkevpeslvivGgGviGvEfasifaklGvkvtvielldrilpaldaevskv 216 +p+ +p +d++v+++s++ale++ vp++l ++G+GviG+E++s++++lG++vtv+e+ld+ l a d+ vsk+ lcl|FitnessBrowser__WCS417:GFF1751 154 RPIDIPP-APVDQNVIVDSTGALEFQAVPKRLGVIGAGVIGLELGSVWSRLGSEVTVLEALDTFLLAADTAVSKE 227 ******9.8999999************************************************************ PP TIGR01350 217 lkkklkkkgvkiltnakvtevekeedevvveakkk.evetleaekvLvavGrkpnleelgleklgveldergaik 290 + k+l+k+g++i+ +a+vt + + +evvv+ +k ++t++ +k++vavGr+p +++l + gv+lderg+++ lcl|FitnessBrowser__WCS417:GFF1751 228 ALKTLTKQGLDIKLGARVTGSKVNGEEVVVNYTDKdGEKTITFDKLIVAVGRRPVTTDLLAADSGVTLDERGYVH 302 *******************88888888886666655679************************************ PP TIGR01350 291 vdeelrtnvpgiyaiGDvigklmLAhvAskegvvaaekiagkekseidykavPsviytePevasvGlteeqakee 365 vd+++ t+vpg+yaiGDv++++mLAh+As+eg++++e+i g+++ +++y+++Psviyt+Pe+a vG++e+q+k+e lcl|FitnessBrowser__WCS417:GFF1751 303 VDDHCATTVPGVYAIGDVVRGMMLAHKASEEGIMVVERIKGHKT-QMNYDLIPSVIYTHPEIAWVGKNEQQLKAE 376 *****************************************666.9***************************** PP TIGR01350 366 gievkvgkfpfaangkalaleetdGfvkvivdkktgeilGahivgaeaseliselalaveleltveelaktihpH 440 g+ev+vg fpfaa+g+a+a+++t Gfvkvi+d kt+++lG+h++g+ a+el+++ a+ +e+++++e++ ++++ H lcl|FitnessBrowser__WCS417:GFF1751 377 GVEVNVGTFPFAASGRAMAANDTGGFVKVIADAKTDRVLGVHVIGPGAAELVQQGAIGMEFGTSAEDIGMMVFSH 451 *************************************************************************** PP TIGR01350 441 PtlsEaikeaalaalgkaihv 461 PtlsEa++eaala++g aih+ lcl|FitnessBrowser__WCS417:GFF1751 452 PTLSEALHEAALAVNGGAIHI 472 *******************95 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (461 nodes) Target sequences: 1 (478 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 7.56 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory