GapMind for catabolism of small carbon sources

 

Alignments for a candidate for mtlK in Pseudomonas simiae WCS417

Align SmoK aka POLK, component of Hexitol (glucitol; mannitol) porter (characterized)
to candidate GFF2270 PS417_11575 spermidine/putrescine ABC transporter ATPase

Query= TCDB::P54933
         (332 letters)



>FitnessBrowser__WCS417:GFF2270
          Length = 342

 Score =  257 bits (657), Expect = 3e-73
 Identities = 143/340 (42%), Positives = 202/340 (59%), Gaps = 14/340 (4%)

Query: 1   MGKITLRNVQKRFGEAVVIPSLDLDIEDGEFVVFVGPSGCGKSTLLRLIAGLEDVSDGQI 60
           M  + L  + KR+G    + + DL +E GEFV  +GPSGCGK+T L++IAG  DVS GQI
Sbjct: 1   MAFLQLNALSKRYGAVDAVVATDLAVEKGEFVSLLGPSGCGKTTTLQMIAGFVDVSGGQI 60

Query: 61  MIDGRDATEMPPAKRGLAMVFQSYALYPHMTVKKNIAFPLRMAKMEPQEIERRVSNAAKI 120
           ++DGRD T   PA RGL +VFQSYAL+PHM+V+ N+AF L+M K+   EI  +V    ++
Sbjct: 61  LLDGRDITHAKPASRGLGVVFQSYALFPHMSVRDNVAFGLKMRKVPAAEIASKVKTVLEL 120

Query: 121 LNLTNYLDRRPGQLSGGQRQRVAIGRAIVREPAAFLFDEPLSNLDAALRVNMRLEITELH 180
           + L  + DR P +LSGGQRQRVA+ RA+V EP   L DEPLSNLDA LR  M+ EI  + 
Sbjct: 121 VRLAPHADRYPRELSGGQRQRVALARALVIEPPVLLLDEPLSNLDANLREEMQFEIRRIQ 180

Query: 181 QSLETTMIYVTHDQVEAMTMADKIVVLNAGRIEQVGSPLTLYRNPANLFVAGFIGSPKMN 240
            ++  T + VTHDQ EA++++D++VV+ AGR+ Q+ +P  LY +P   F++ F+G  K N
Sbjct: 181 CAVGITTLMVTHDQAEALSISDRVVVMQAGRVTQIDAPYKLYEHPRTRFISDFVG--KAN 238

Query: 241 LIEGP----------EAAKHGATTIGIRPEHIDL-SREAGAWEGEVGVSEHLGSDTFLHV 289
           L+ G             +  G  T+ +RPE I L    +G  +G+V  S   GS     +
Sbjct: 239 LLPGDYDLLGSPQVRHESGDGELTLSLRPEKIQLVDAGSGRLQGKVLDSYFFGSQWLYRI 298

Query: 290 HVA-GMPTLTVRTGGEFGVHHGDRVWLTPQADKIHRFGAD 328
           H + G  T+     G   + +G  V L  QA  +     D
Sbjct: 299 HTSLGEITVVRSNDGSAPLGYGAAVGLDWQASLLRVLAKD 338


Lambda     K      H
   0.320    0.137    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 319
Number of extensions: 9
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 332
Length of database: 342
Length adjustment: 28
Effective length of query: 304
Effective length of database: 314
Effective search space:    95456
Effective search space used:    95456
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory