Alignments for a candidate for HSERO_RS03640 in Pseudomonas simiae WCS417

GapMind for catabolism of small carbon sources

Alignments for a candidate for HSERO_RS03640 in Pseudomonas simiae WCS417

Align Ribose import ATP-binding protein RbsA; EC 7.5.2.7 (characterized, see rationale)
to candidate GFF4164 PS417_21330 L-arabinose transporter ATP-binding protein

Query= uniprot:D8IZC7
         (521 letters)



>FitnessBrowser__WCS417:GFF4164
          Length = 499

 Score =  377 bits (969), Expect = e-109
 Identities = 206/494 (41%), Positives = 306/494 (61%), Gaps = 9/494 (1%)

Query: 7   LQMRGIRKSFGATLALSDMHLTIRPGEIHALMGENGAGKSTLMKVLSGVHAPDQGEILLD 66
           L+  GI K F    ALS +    RP E+HALMGENGAGKSTL+K+L G + P  G + + 
Sbjct: 6   LRFNGIGKEFPGVKALSQISFEARPREVHALMGENGAGKSTLLKILGGAYLPSSGTLQIG 65

Query: 67  GRPVALRDPGASRAAGINLIYQELAVAPNISVAANVFMGSELRTRLGLIDHAAMRSRTDA 126
            + +  +    S A G+ +I+QEL + P ++VA N+F+G  L TR G+++ + +R +  A
Sbjct: 66  EQTMDFKSAADSIACGVAVIHQELHLVPEMTVAENLFLG-HLPTRFGVVNRSQLRKQALA 124

Query: 127 VLRQLGAGFGASDLAGRLSIAEQQQVEIARALVHRSRIVIMDEPTAALSERETEQLFNVV 186
            L+ L       +  GRLS+ ++Q VEIA+AL   + ++  DEPT++LS RE ++L  ++
Sbjct: 125 CLKGLADEIDPDEKLGRLSLGQRQLVEIAKALSRGAHVIAFDEPTSSLSAREIDRLMAII 184

Query: 187 RRLRDEGLAIIYISHRMAEVYALADRVTVLRDGSFVGELV-RDEIDSERIVQMMVGRSLS 245
            RLRDEG  ++Y+SHRM EV+ + + VTV +DG +V      + +  +++V  MVGR + 
Sbjct: 185 TRLRDEGKVVLYVSHRMEEVFRICNAVTVFKDGRYVRTFDDMNALSHDQLVTCMVGRDIQ 244

Query: 246 EFYQHQRIAPADAAQLPTVMQVRALAGGKIR-PASFDVRAGEVLGFAGLVGAGRTELARL 304
           + Y +++    + A     ++V  L G  +R P S +V  GE+LG  GLVGAGRTEL RL
Sbjct: 245 DIYDYRQREQGEVA-----LKVEGLLGPGLREPVSLNVHKGEILGLFGLVGAGRTELFRL 299

Query: 305 LFGADPRSGGDILLEGRPVHIDQPRAAMRAGIAYVPEDRKGQGLFLQMAVAANATMNVAS 364
           L G    + G + L G+ + +  PR A+ AG+   PEDRK +G+    +VA N  ++   
Sbjct: 300 LSGLTRSTAGSLALCGQTLQLRSPRDAIAAGVLLCPEDRKKEGIIPLSSVAENINISARG 359

Query: 365 RHTRLG-LVRSRSLGGVARAAIQRLNVKVAHPETPVGKLSGGNQQKVLLARWLEIAPKVL 423
            H+  G L+R       A   I+ + VK  + E  +  LSGGNQQK +L RWL +  KVL
Sbjct: 360 AHSTFGWLLRDGWETTNADRQIKAMKVKTPNAEQKIMYLSGGNQQKAILGRWLSMPMKVL 419

Query: 424 ILDEPTRGVDIYAKSEIYQLVHRLASQGVAVVVISSELPEVIGICDRVLVMREGMITGEL 483
           +LDEPTRG+DI AKSEIYQ++H LA+ G+AV+V+SS+L EV+GI DR+LVM EG +TGEL
Sbjct: 420 LLDEPTRGIDIGAKSEIYQIIHNLAASGIAVIVVSSDLMEVMGISDRILVMSEGALTGEL 479

Query: 484 AGAAITQENIMRLA 497
                 +  +++LA
Sbjct: 480 TRDQADEARLLQLA 493



 Score = 96.7 bits (239), Expect = 2e-24
 Identities = 63/223 (28%), Positives = 114/223 (51%), Gaps = 6/223 (2%)

Query: 27  LTIRPGEIHALMGENGAGKSTLMKVLSGVHAPDQGEILLDGRPVALRDPGASRAAGINLI 86
           L +  GEI  L G  GAG++ L ++LSG+     G + L G+ + LR P  + AAG+ L 
Sbjct: 275 LNVHKGEILGLFGLVGAGRTELFRLLSGLTRSTAGSLALCGQTLQLRSPRDAIAAGVLLC 334

Query: 87  YQEL---AVAPNISVAANVFMGSE-LRTRLG-LIDHAAMRSRTDAVLRQLGAGF-GASDL 140
            ++     + P  SVA N+ + +    +  G L+      +  D  ++ +      A   
Sbjct: 335 PEDRKKEGIIPLSSVAENINISARGAHSTFGWLLRDGWETTNADRQIKAMKVKTPNAEQK 394

Query: 141 AGRLSIAEQQQVEIARALVHRSRIVIMDEPTAALSERETEQLFNVVRRLRDEGLAIIYIS 200
              LS   QQ+  + R L    +++++DEPT  +      +++ ++  L   G+A+I +S
Sbjct: 395 IMYLSGGNQQKAILGRWLSMPMKVLLLDEPTRGIDIGAKSEIYQIIHNLAASGIAVIVVS 454

Query: 201 HRMAEVYALADRVTVLRDGSFVGELVRDEIDSERIVQMMVGRS 243
             + EV  ++DR+ V+ +G+  GEL RD+ D  R++Q+ + RS
Sbjct: 455 SDLMEVMGISDRILVMSEGALTGELTRDQADEARLLQLALPRS 497


Lambda     K      H
   0.320    0.135    0.378 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 649
Number of extensions: 33
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 521
Length of database: 499
Length adjustment: 34
Effective length of query: 487
Effective length of database: 465
Effective search space:   226455
Effective search space used:   226455
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources