Alignments for a candidate for iatA in Pseudomonas simiae WCS417

GapMind for catabolism of small carbon sources

Alignments for a candidate for iatA in Pseudomonas simiae WCS417

Align Inositol transport ATP-binding protein IatA, component of The myoinositol (high affinity)/ D-ribose (low affinity) transporter IatP/IatA/IbpA. The structure of IbpA with myoinositol bound has been solved (characterized)
to candidate GFF2162 PS417_11030 L-arabinose transporter ATP-binding protein

Query= TCDB::B8H229
         (515 letters)



>FitnessBrowser__WCS417:GFF2162
          Length = 500

 Score =  384 bits (987), Expect = e-111
 Identities = 218/488 (44%), Positives = 316/488 (64%), Gaps = 9/488 (1%)

Query: 9   VSKSFPGVRALDQVDLVVGVGEVHALLGENGAGKSTLIKILSAAHAADAGTVTFAGQVLD 68
           + K+FPGV+ALD +      G+VHAL+GENGAGKSTL+KIL  A+  ++GT+    QV+ 
Sbjct: 11  IGKTFPGVKALDGISFSAHPGQVHALMGENGAGKSTLLKILGGAYIPNSGTLHIGEQVMA 70

Query: 69  PRDAPLRRQQLGIATIYQEFNLFPELSVAENMYLGREPRRLGLVDWSRLRADAQALLNDL 128
            + A       G+A I+QE +L PE+SVAEN++LG  P R G+V+   LR  A A L  L
Sbjct: 71  FKSAADSIAS-GVAVIHQELHLVPEMSVAENLFLGHLPSRFGVVNRGLLRKQALACLKGL 129

Query: 129 GLPLNPDAPVRGLTVAEQQMVEIAKAMTLNARLIIMDEPTAALSGREVDRLHAIIAGLKA 188
              ++P   +  L++ ++Q+VEIAKA++  A +I  DEPT++LS RE+DRL AIIA L+ 
Sbjct: 130 ADEIDPAEKLGRLSLGQRQLVEIAKALSRGAHVIAFDEPTSSLSAREIDRLMAIIARLRD 189

Query: 189 RSVSVIYVSHRLGEVKAMCDRYTVMRDGRFVAS-GDVADVEVADMVRLMVGRHVEFERRK 247
               V+YVSHR+ EV  +C+  TV +DGR+V +  D++ +    +V  MVGR ++     
Sbjct: 190 EGKVVLYVSHRMEEVFRICNAVTVFKDGRYVRTFEDMSALTHDQLVTCMVGRDIQDIYDY 249

Query: 248 RRRPPGAVVLKVEGVTPAAPRLSAPGYLRQVSFAARGGEIVGLAGLVGAGRTDLARLIFG 307
           R R  G V LKV+G       L  PG    VSF  R GEI+GL GLVGAGRT+L RL+ G
Sbjct: 250 RPREHGEVALKVDG-------LLGPGLREPVSFNVRKGEILGLFGLVGAGRTELFRLLSG 302

Query: 308 ADPIAAGRVLVDDKPLRLRSPRDAIQAGIMLVPEDRKQQGCFLDHSIRRNLSLPSLKALS 367
            +  +AG++ +  +PL L+SPRDAI AG++L PEDRK++G     S+  N+++ +  A S
Sbjct: 303 LERASAGQLELCGEPLHLQSPRDAIAAGVLLCPEDRKKEGIIPLSSVAENINISARGAHS 362

Query: 368 ALGQWVDERAERDLVETYRQKLRIKMADAETAIGKLSGGNQQKVLLGRAMALTPKVLIVD 427
             G  + E  E+   +     +++K  +A   I  LSGGNQQK +LGR +++  KVL++D
Sbjct: 363 TFGWLLREGWEKGNADQQINAMKVKTPNAAQKIMYLSGGNQQKSILGRWLSMPMKVLLLD 422

Query: 428 EPTRGIDIGAKAEVHQVLSDLADLGVAVVVISSELAEVMAVSDRIVVFREGVIVADLDAQ 487
           EPTRGIDIGAKAE++Q++ +LA  G+AV+V+SS+L EVM ++DRI+V  EG +  +   +
Sbjct: 423 EPTRGIDIGAKAEIYQIIHNLAAQGIAVIVVSSDLMEVMGIADRILVLCEGALRGEQTRE 482

Query: 488 TATEEGLM 495
            ATE  L+
Sbjct: 483 HATESNLL 490



 Score = 79.3 bits (194), Expect = 3e-19
 Identities = 65/261 (24%), Positives = 115/261 (44%), Gaps = 32/261 (12%)

Query: 254 AVVLKVEGVTPAAPRLSAPGYLRQVSFAARGGEIVGLAGLVGAGRTDLARLIFGADPIAA 313
           A  L+ +G+    P + A   L  +SF+A  G++  L G  GAG++ L +++ GA    +
Sbjct: 3   AAALRFDGIGKTFPGVKA---LDGISFSAHPGQVHALMGENGAGKSTLLKILGGAYIPNS 59

Query: 314 GRVLVDDKPLRLRSPRDAIQAGIM-------LVPEDRKQQGCFLDHSIRR----NLSLPS 362
           G + + ++ +  +S  D+I +G+        LVPE    +  FL H   R    N  L  
Sbjct: 60  GTLHIGEQVMAFKSAADSIASGVAVIHQELHLVPEMSVAENLFLGHLPSRFGVVNRGLLR 119

Query: 363 LKALSALGQWVDERAERDLVETYRQKLRIKMADAETAIGKLSGGNQQKVLLGRAMALTPK 422
            +AL+ L    DE                   D    +G+LS G +Q V + +A++    
Sbjct: 120 KQALACLKGLADE------------------IDPAEKLGRLSLGQRQLVEIAKALSRGAH 161

Query: 423 VLIVDEPTRGIDIGAKAEVHQVLSDLADLGVAVVVISSELAEVMAVSDRIVVFREGVIVA 482
           V+  DEPT  +       +  +++ L D G  V+ +S  + EV  + + + VF++G  V 
Sbjct: 162 VIAFDEPTSSLSAREIDRLMAIIARLRDEGKVVLYVSHRMEEVFRICNAVTVFKDGRYVR 221

Query: 483 DLDAQTATEEGLMAYMATGTD 503
             +  +A     +     G D
Sbjct: 222 TFEDMSALTHDQLVTCMVGRD 242



 Score = 68.9 bits (167), Expect = 4e-16
 Identities = 63/236 (26%), Positives = 107/236 (45%), Gaps = 12/236 (5%)

Query: 14  PGVRALDQVDLVVGVGEVHALLGENGAGKSTLIKILSAAHAADAGTVTFAGQVL---DPR 70
           PG+R  + V   V  GE+  L G  GAG++ L ++LS    A AG +   G+ L    PR
Sbjct: 267 PGLR--EPVSFNVRKGEILGLFGLVGAGRTELFRLLSGLERASAGQLELCGEPLHLQSPR 324

Query: 71  DAPLRRQQLGIATIYQEFNLFPELSVAENMYLGREPRR-----LGLVDWSRLRADAQALL 125
           DA +    L      ++  + P  SVAEN+ +           L    W +  AD Q   
Sbjct: 325 DA-IAAGVLLCPEDRKKEGIIPLSSVAENINISARGAHSTFGWLLREGWEKGNADQQINA 383

Query: 126 NDLGLPLNPDAPVRGLTVAEQQMVEIAKAMTLNARLIIMDEPTAALSGREVDRLHAIIAG 185
             +  P N    +  L+   QQ   + + +++  +++++DEPT  +       ++ II  
Sbjct: 384 MKVKTP-NAAQKIMYLSGGNQQKSILGRWLSMPMKVLLLDEPTRGIDIGAKAEIYQIIHN 442

Query: 186 LKARSVSVIYVSHRLGEVKAMCDRYTVMRDGRFVASGDVADVEVADMVRLMVGRHV 241
           L A+ ++VI VS  L EV  + DR  V+ +G             +++++L + R V
Sbjct: 443 LAAQGIAVIVVSSDLMEVMGIADRILVLCEGALRGEQTREHATESNLLQLALPRSV 498


Lambda     K      H
   0.320    0.136    0.380 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 674
Number of extensions: 30
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 3
Number of HSP's successfully gapped: 3
Length of query: 515
Length of database: 500
Length adjustment: 34
Effective length of query: 481
Effective length of database: 466
Effective search space:   224146
Effective search space used:   224146
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources