Align Maleylacetoacetate isomerase; MAAI; GSTZ1-1; Glutathione S-transferase zeta 1; EC 5.2.1.2; EC 2.5.1.18 (characterized)
to candidate GFF3510 PS417_17970 glutathione S-transferase
Query= SwissProt::P57113 (216 letters) >FitnessBrowser__WCS417:GFF3510 Length = 206 Score = 67.4 bits (163), Expect = 2e-16 Identities = 59/195 (30%), Positives = 93/195 (47%), Gaps = 8/195 (4%) Query: 8 LYSYFRSSCSWRVRIALALKGIDYEIVPINLIKDGGQQFSEEFQTLNPMKQVPALKIDGI 67 LY + S S RV + L+L G+ E V ++L K G + E ++NP QVPA+ +G+ Sbjct: 4 LYGFPLSGHSHRVELMLSLLGLPTEFVQVDL-KQGAHKAPEFIASINPFGQVPAIDDNGV 62 Query: 68 TIGQSLAILEYLEETRPIPRLLPQDPQKRAIVRMISDLIASGIQPLQNLSVLKQV-GQEN 126 + S AIL YL T + LP DP +A V+ A + + L V G E Sbjct: 63 VLADSNAILVYLANTYGNGQWLPSDPVGQARVQRWLSAAAGQLHAGPASARLATVFGAEV 122 Query: 127 QMPWAQKAITSGFNALEKIL--QSTAGKYCVGDEVSMADVCLAPQVANAERFKVDLSPYP 184 A + +AL K++ Q + ++ VG++ ++ADV A+A V L+ YP Sbjct: 123 DTAVA----IARSHALLKLVEAQLSQSRFLVGEQPTIADVAFYTYTAHAPEGNVSLADYP 178 Query: 185 TISHINKALLALEAF 199 + ++ AL F Sbjct: 179 QVRAWLASIEALPGF 193 Lambda K H 0.319 0.135 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 84 Number of extensions: 3 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 216 Length of database: 206 Length adjustment: 21 Effective length of query: 195 Effective length of database: 185 Effective search space: 36075 Effective search space used: 36075 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 45 (21.9 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory