GapMind for catabolism of small carbon sources

 

Alignments for a candidate for aapP in Pseudomonas simiae WCS417

Align AapP, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized)
to candidate GFF1093 PS417_05545 amino acid ABC transporter ATP-binding protein

Query= TCDB::Q52815
         (257 letters)



>FitnessBrowser__WCS417:GFF1093
          Length = 244

 Score =  285 bits (730), Expect = 5e-82
 Identities = 144/241 (59%), Positives = 180/241 (74%), Gaps = 2/241 (0%)

Query: 18  VEIVNMNKWYGDFHVLRDINLKVMRGERIVIAGPSGSGKSTMIRCINRLEEHQKGKIVVD 77
           + I N+NKWYGDF VL D +  V +GE IV+ GPSGSGKST+I+C+N LE  QKG IVVD
Sbjct: 2   ISIKNINKWYGDFQVLTDCSTDVKKGEVIVVCGPSGSGKSTLIKCVNALEPFQKGDIVVD 61

Query: 78  GTELTNDLKKIDEVRREVGMVFQHFNLFPHLTILENCTLAPIWVRKMPKKQAEEVAMHFL 137
           GT + +    + ++R  VGMVFQHF LFPHLTI EN T+A I V    K++A +  +  L
Sbjct: 62  GTSIADPKTNLPQLRSRVGMVFQHFELFPHLTITENLTIAQIKVLGRSKEEATKKGLQLL 121

Query: 138 KRVKIPEQANKYPGQLSGGQQQRVAIARSLCMNPKIMLFDEPTSALDPEMIKEVLDTMVG 197
           +RV +   A+K+PGQLSGGQQQRVAIAR+L M+P +MLFDEPTSALDPEM+ EVLD MV 
Sbjct: 122 ERVGLSAHAHKHPGQLSGGQQQRVAIARALAMDPIVMLFDEPTSALDPEMVNEVLDVMVQ 181

Query: 198 LAEEGMTMLCVTHEMGFARQVANRVIFMDQGQIVEQNEPAAFFD--NPQHERTKLFLSQI 255
           LA+EGMTM+CVTHEMGFAR+VA+RVIFMD G+I+E      FF   + + ER + FL +I
Sbjct: 182 LAQEGMTMMCVTHEMGFARKVADRVIFMDAGKIIEDCPKEEFFGDISARSERAQHFLEKI 241

Query: 256 L 256
           L
Sbjct: 242 L 242


Lambda     K      H
   0.321    0.135    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 215
Number of extensions: 9
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 257
Length of database: 244
Length adjustment: 24
Effective length of query: 233
Effective length of database: 220
Effective search space:    51260
Effective search space used:    51260
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory