Alignments for a candidate for prpE in Pseudomonas simiae WCS417

GapMind for catabolism of small carbon sources

Alignments for a candidate for prpE in Pseudomonas simiae WCS417

Align Acyl-coenzyme A synthetase ACSM3, mitochondrial; Acyl-CoA synthetase medium-chain family member 3; Butyrate--CoA ligase 3; Butyryl-coenzyme A synthetase 3; Middle-chain acyl-CoA synthetase 3; Propionate--CoA ligase; Protein SA homolog; EC 6.2.1.2; EC 6.2.1.17 (characterized)
to candidate GFF2136 PS417_10895 AMP-dependent synthetase

Query= SwissProt::Q3UNX5
         (580 letters)



>FitnessBrowser__WCS417:GFF2136
          Length = 566

 Score =  390 bits (1002), Expect = e-113
 Identities = 214/546 (39%), Positives = 317/546 (58%), Gaps = 26/546 (4%)

Query: 35  SNYESMKQDFKIEIPEYFNFAKDVLDQWTNMEKAGKRLSNPAFWWIDGNGEELRWSFEEL 94
           ++Y +  +DF+      FN+A D  D       A       A W ++ +G E R+SF++L
Sbjct: 17  TDYATAVRDFRWPQLGEFNWALDYFDAMAEGNPAD------ALWIVEEDGSEQRYSFQQL 70

Query: 95  GLLSRKFANILTEACSLQRGDRVMVILPKIPEWWLANVACLRTGTVLIPGTTQLTQKDIL 154
              S + AN L  A  + RGDRV+++L      W   +A  + G V+IP T  L   D+ 
Sbjct: 71  AARSNQVANHL-RALGVNRGDRVLLMLGNDVALWDTMLAAFKLGAVVIPATALLNPDDLR 129

Query: 155 YRLQSSKAKCIITDDTLAPAVDAVAAKCENLHSKLIVSQHSREGWGNLKEMMKYASDSHT 214
            R++  + + ++  +        +A  C    S++ V   +  GW       +Y      
Sbjct: 130 DRIERGQVRHVVVGEAHVHKFAGLAQGC----SRICVGS-APAGWVAHNAAFEYPEQFEA 184

Query: 215 CVDTKHDEMMAIYFTSGTTGPPKMIGHTHSSFGLGLSVNGRFWLDLIASDVMWNTSDTGW 274
              T   + M +YFTSGTT  PKM+ H+H S+ +G  ++  +W+ L   D+  N S  GW
Sbjct: 185 QGRTLATDPMLLYFTSGTTSKPKMVLHSHQSYPVG-HLSTMYWIGLQPGDLHLNISSPGW 243

Query: 275 AKSAWSSVFSPWTQGACVFAHYLPRFESTSILQTLSKFPITVFCSAPTAYRMLVQNDMSS 334
           AK AWS +F+PW  GAC+F H + RF + ++L  L ++ +T  C+ PT +RML+Q D++S
Sbjct: 244 AKHAWSCLFAPWNAGACIFIHNVARFSAPALLSALERYGVTSLCAPPTVWRMLIQEDLAS 303

Query: 335 YKFN-SLKHCVSAGEPINPEVMEQWRKKTGLDIYEGYGQTETVLICGNFKGMKIKPGSMG 393
           Y+   SL+  V AGEP+NPE++EQ ++  GL + +G+GQ+ET  + GN  G  +KPGSMG
Sbjct: 304 YRPRLSLRELVGAGEPLNPEIIEQIQQAWGLPLRDGFGQSETTALVGNTPGQLLKPGSMG 363

Query: 394 KPSPAFDVKILDENGATLPPGQEGDIALQVLPERPFGLFTHYVDNPSKTASTLRGSFYIT 453
           +P P + V +LD +G    PG EG++AL  L  RP GL   Y D+P KTA  +R  +Y T
Sbjct: 364 RPLPGYQVALLDPDGI---PGNEGEVALP-LDVRPLGLMLCYEDSPEKTAEVMRDGYYRT 419

Query: 454 GDRGYMDEDGYFWFVARSDDIILSSGYRIGPFEVESALIEHPSIAESAVVSSPDPIRGEV 513
           GD   +D DGY  FV R+DD+  +S YRI PFE+ESALIEHP++ E AVV SPDP+R  V
Sbjct: 420 GDTAQIDADGYITFVGRADDVFKASDYRISPFELESALIEHPAVMEVAVVPSPDPLRLAV 479

Query: 514 VKAFIVLNPDYKSHDQ---EQLKKEIQEHVKKTTAPYKYPRKVEFIEELPKTVSGKVKRN 570
            KAF++L     +HD+     L + I    ++  APYK  R++EF+ ELPKT+SGK++R 
Sbjct: 480 PKAFLIL-----AHDEPGSAALAQHILAFAREHLAPYKRVRRIEFVTELPKTISGKIRRV 534

Query: 571 ELRKKE 576
           ELR+ E
Sbjct: 535 ELRQME 540


Lambda     K      H
   0.319    0.134    0.416 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 876
Number of extensions: 48
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 580
Length of database: 566
Length adjustment: 36
Effective length of query: 544
Effective length of database: 530
Effective search space:   288320
Effective search space used:   288320
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources